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12. Effect of iron, zinc and manganese shortage-induced change on photosynthetic pigments, some osmoregulators and chlorophyll fluorescence parameters in lettuce

Creator:
Roosta, H. R., Estaji, A., and Niknam, F.
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
železo, zinek, mangan, iron, zinc, manganese, mikronutrienty, locika setá, kvantový výtěžek, fotosyntetické pigmenty, chlorophyll fluorescence transients, mikronutrients, Lactuca sativa, quantum yield, photosynthetic pigments, 2, and 581
Language:
Multiple languages
Description:
Although the beneficial role of Fe, Zn, and Mn on many physiological and biochemical processes is well established, effects of each of these elements on chlorophyll (Chl) a fluorescence and photosynthetic pigment contents is not well studied. The objective of this study was to evaluate effects of Fe, Zn, and Mn deficiency in two lettuce cultivars. The parameters investigated could serve also as physiological and biochemical markers in order to identify stress-tolerant cultivars. Our results indicated that microelement shortage significantly decreased contents of photosynthetic pigments in both lettuce cultivars. Chl a fluorescence parameters including maximal quantum yield of PSII photochemistry and performance index decreased under micronutrient deficiency, while relative variable fluorescence at J-step and minimal fluorescence yield of the dark-adapted state increased under such conditions in both cultivars. Micronutrient deficiency also reduced all parameters of quantum yield and specific energy fluxes excluding quantum yield of energy dissipation, quantum yield of reduction of end electron acceptors at the PSI, and total performance index for the photochemical activity. Osmoregulators, such as proline, soluble sugar, and total phenols were enhanced in plants grown under micronutrient deficiency. Fe, Zn, and Mn deficiency led to a lesser production of dry mass. The Fe deficiency was more destructive than that of Zn and Mn on the efficiency of PSII in both lettuce cultivars. Our results suggest that the leaf lettuce, which showed a higher efficiency of PSII, electron transport, quantum yield, specific energy fluxes, and osmoregulators under micronutrient deficiency, was more tolerant to stress conditions than crisphead lettuce., H. R. Roosta, A. Estaji, F. Niknam., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

13. Effect of sodium nitroprusside on responses of Melissa officinalis to bicarbonate exposure and direct Fe deficiency stress

Creator:
Amooaghaie, R. and Roohollahi, Sh.
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
železo, oxid dusnatý, iron, nitric oxide, hydrogenuhličitan, citrónový balzám, fluorescence chlorofylu, meduňka lékařská, bicarbonate, lemon balm, chlorophyll fluorescence, 2, and 581
Language:
Multiple languages
Description:
In our study, one-month-old Melissa officinalis plants were subjected to Fe-deficiency treatments, such as 10 µM Fe (as direct iron deficiency, DD), and 30 µM Fe + 10 mM NaHCO3 + 0.5 g l-1 CaCO3 (as indirect iron deficiency, ID), and 30 µM Fe (as control) for 14 d. Both Fe-deficiency types reduced plant growth, photosynthetic pigment contents, an active Fe content in roots and leaves, root Fe(III)-reducing capacity, Fe-use efficiency, maximal quantum yield of PSII photochemistry, a ratio of variable to basic fluorescence, and activities of antioxidant enzymes, while they increased lipid peroxidation and a H2O2 content in leaves. These effects were more pronounced in plants exposed to ID with bicarbonate than those of DD plants. We showed that sodium nitroprusside (SNP), as NO donor, could ameliorate the adverse effects of bicarbonate on above traits. The methylene blue, as NO blocker, reversed the protective effects conferred by SNP in the ID-treated plants as well as DD plants. These findings suggests that NO protects photosynthesis and growth of IDtreated plants as well as DD plants by contribution in availability and/or delivery of metabolically active iron or by changing activities of reactive oxygen species-scavenging enzymes., R. Amooaghaie, Sh. Roohollahi., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

15. Hepcidin expression in adipose tissue increases during cardiac surgery

Creator:
Martin Vokurka, Zdeňka Lacinová, Jaromír Křemen, Kopecký, P., Jan Bláha, Květa Pelinková, Haluzík, M., and Emanuel Nečas
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, železo, záněty, chirurgie, iron, inflammations, surgery, hepcidin, adipose tissue, 14, and 612
Language:
English
Description:
Hepcidin, a key regulator of iron metabolism, plays a crucial role in the pathogenesis of anemia of chronic disease. Although it is produced mainly in the liver, its recently described expression in adipose tissue has been shown to be enhanced in massive obesity due to chronic low-grade inflammation. Our objective was to study the changes in hepcidin expression in adipose tissue during acute-phase reaction. We measured hepcidin mRNA expression from isolated subcutaneous and epicardial adipose tissue at the beginning and at the end of the surgery. The expression of mRNAs for hepcidin and other iron-related genes (transferrin receptor 1, divalent metal transporter 1, ferritin, ferroportin) were measured by real-time RT-PCR. Hepcidin expression significantly increased at the end of the surgery in subcutaneous but not in epicardial adipose tissue. Apart from the increased levels of cytokines, the parameters of iron metabolism showed typical inflammation-induced changes. We suggest that acute inflammatory changes could affect the regulation of hepcidin expression in subcutaneous adipose tissue and thus possibly contribute to inflammation-induced systemic changes of iron metabolism., M. Vokurka ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

16. In vitro assessment of iron effect on porcine ovarian granulosa cells: secretory activity, markers of proliferation and apoptosis

Creator:
Kolesarova, A., Capcarova, M., Medvedova, M., Alexander V. Sirotkin, and Kovacik, J.
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, železo, progesteron, apoptóza, iron, progesterone, apoptosis, IGF-I, proliferation, granulosa cell, 14, and 612
Language:
English
Description:
t would be desirable to expand the existing general knowledge concerning direct action of metals on the ovary. Nevertheless, the results of testing of iron compound on porcine ovarian cells should be interpreted carefully because iron is an essential element which could also induce changes in cellular processes. The aim of this in vitro study was 1) to examine dose-dependent effects of iron on the secretory activity of porcine ovarian granulosa cells, and 2) to outline the potential intracellular mediators mediating these effects. Specifically, we evaluated the effect of iron sulphate on the release of insulin-like growth factor I (IGF-I) and progesterone, as well as the expression of markers of proliferation (cyclin B1) and apoptosis (caspase-3) in porcine ovarian granulosa cells. Concentrations of IGF-I and progesterone were determined by RIA, cyclin B1 and caspase-3 expression by immunocytochemistry (ICC). Our results show a significantly decreased IGF-I secretion by ovarian granulosa cells after iron sulphate addition at the doses 0.5 and 1.0 mg/ml. The iron sulphate additions at do ses 0.17 and 1.0 mg/ml had no effect on progesterone secretion. In contrast, iron sulphate addition at doses 0.17-1.0 mg/ml resulted in stimulation of cyclin B1 and caspase-3 expression. In conclusion, the present results indicate a direct effect of iron on 1) secretion of growth factor IGF-I but not steroid hormone progesterone, 2) expression of markers of proliferation (cyclin B1), or 3) apoptosis (caspase-3) of porcine ovarian granulosa cells. These results support an idea that iron could play a regulatory role in porcine ovarian function: hormone release, prolif eration and apoptosis., A. Kolesarova ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public