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42. Structure elucidation of Mas-AKH as the major adipokinetic hormone in the butterfly Vanessa cardui (Lepidoptera: Nymphalidae)
- Creator:
- Gäde, Gerd, Hoffmann, Klaus H., Köllisch, Gabriele, Verhaert, Peter D., Lorenz, Matthias W., and Kellner, Roland
- Format:
- print, text, and regular print
- Type:
- article, bibliography, model:article, and TEXT
- Subject:
- zoologie, entomologie, hmyz, motýli, Nymphalidae, Vanessa cardui, corpora cardiaca, adipokinetické hormony, struktura, peptidické složení, hmotnostní spektrometrie, 595.2/.7, and 591.1
- Language:
- English
- Description:
- The presence of adipokinetic activity in crude extracts of corpora cardiaca (CC) from the butterfly (Vanessa cardui L., Nymphalidae) was demonstrated by bioassay and Mas-AKH was revealed as the major adipokinetic hormone (AKH) by use of two different technologies of sequence elucidation: HPLC separation of the peptide followed by Edman degradation and Q-TOF mass spectrometry. In contrast to the time- and material-consuming conventional methods of peptide purification and sequencing, substantial structural data of the peptide were confirmed - post factum - from one pCC (pair of CC) by Q-TOF mass spectrometry. Only males of our laboratory colony showed a significant lipid increase in the haemolymph after injection of either crude CC extract (1 pCC equivalent) or 10 pmol of synthetic peptide., Gabriele Köllisch, Peter D. Verhaert, Matthias W. Lorenz, Roland Kellner, Gerd Gäde, Klaus H. Hoffmann, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
43. Temperature, development and establishment potential of Thrips palmi (Thysanoptera: Thripidae) in the United Kingdom
- Creator:
- McDonald, Jamie R., Bale, Jeffrey S., and Walters, Keith F.A.
- Format:
- print, text, and regular print
- Type:
- article, bibliography, conference publication, model:article, and TEXT
- Subject:
- Third European Workshop of Invertebrate Ecophysiology (1998 : Birmingham, Česko), zoologie, fyziologie živočichů, ekofyziologie, hmyz, Thysanoptera, Thripidae, Thrips palmi, Frankliniella occidentalis, vývoj, přezimování, teplota, teplotní biologie, 591.5, 061.3, and 595.2/.7
- Language:
- English
- Description:
- In order to manage the risks posed to domestic crop production by quarantine pests such as Thrips palmi, their potential to establish in a new environment must be assessed. The thermal requirements for development of T. palmi were determined and compared with UK temperatures, to estimate its potential for development under UK conditions. Temperature and rate of development of T. palmi from egg to adult were linearly related between 15 and 30°C, allowing calculation of an overall threshold of 10.1°C, and a sum of effective temperatures of 194 degree-days. In the UK, development of T. palmi would be possible outdoors during the summer when a maximum of up to four or five generations could occur. Comparison of these data with those of the recently established and biologically similar pest, Frankliniella occidentalis, shows that establishment of T. palmi in the UK is unlikely to be limited by an inability to complete the life cycle during the favourable season., Jamie R. McDonald, Jeffrey S. Bale, Keith F.A. Walters, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
44. The influence of thermal acclimation on the amylolytic activity and microanatomy of the alimentary tract of the oribatid mite Galumna elimata (Acari: Oribatei)
- Creator:
- Šustr, Vladimír and Jan Hubert
- Format:
- print, text, and regular print
- Type:
- article, bibliography, conference publication, model:article, and TEXT
- Subject:
- Third European Workshop of Invertebrate Ecophysiology (1998 : Birmingham, Česko), zoologie, fyziologie živočichů, ekofyziologie, roztoči, Oribatei, Galumna elimata, trávicí trakt, trávicí enzymy, amylolytická aktivita, teplota, teplotní aklimatizace, 591.5, 061.3, and 595.2/.7
- Language:
- English
- Description:
- The oribatid mite Galumna elimata was reared under laboratory conditions on algae (Protacoccus spp.) at different temperatures (5, 15 and 20°C). Higher weight-specific amylolytic activity was found in the whole body homogenates of mites exposed to cold acclimation (5°C, 21 days) in comparison with individuals acclimated to 15 and 20°C. Accompanying parameters (live body weight, content of total soluble proteins in the body, protein-specific amylolytic activity, presence and composition of food boluses, activity of mesenteric and caecal wall cells, gregarinid parasitisation, number of glycogen granulae and guanine deposits in mesenchymal tissue, presence of proventricular glands) were investigated at all three acclimation temperatures to explain mechanisms of this change. The acclimation response of weight-specific amylolytic activity was accompanied by elevation of protein-specific amy lolytic activity. Microanatomical features did not confirm any increased secretion activity of mesenteric and caecal cells in cold acclimated animals. Granulation and apocrine secretion of these cells increased with increasing temperature as with food ingestion. The food boluses contained algae or algae mixed with unknown fungal hyphae in individuals acclimated to 15 and 20°C. The concentrated mucoid substances prevailed in animals exposed to cold, indicating lower feeding activity at 5°C. Based on these findigs, Lye hypothesised that specific amylolytic activity reflected passively the different changes in protein composition of the body at different temperatures and its elevation is without direct adaptive importance., Vladimír Šustr, Jan Hubert, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
45. The male of Tetanoptera leucodactyla (Diptera: Sciomyzidae)
- Creator:
- Knutson, Lloyd V. and Vala, Jean-Claude
- Format:
- print, text, and regular print
- Type:
- article, bibliography, model:article, and TEXT
- Subject:
- zoologie, entomologie, hmyz, dvoukřídlí, Sciomyzidae, Tetanoptera leucodactyla, Verbekaria, taxonomie, Afrika, and 595.2/.7
- Language:
- English
- Description:
- The male of Tetanoptera leucodactyla Verbèke, a genus and species of Sciomyzidae known until now only from one female specimen from the Congo, is described, and the genus is more fully characterized. The external morphology is illustrated and the male genitalia are described and figured for the first time. The relationships of Tetanoptera to genera of the Sepedon group and certain other genera of Tetanocerini are discussed. Tetanoptera appears to be related to Verbekaria, and intermediate in an evolutionary lineage between the relatively primitive Tetanocera and the advanced Thecomyia., Lloyd V. Knutson, Jean-Claude Vala, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
46. Tingidae (Hemiptera) of Vanuatu (New Hebrides): New species and new records
- Creator:
- Guilber, Eric
- Format:
- print, text, and regular print
- Type:
- article, bibliography, model:article, and TEXT
- Subject:
- zoologie, entomologie, hmyz, Hemiptera, Tingidae, nové druhy, taxonomický popis, klíč k určování, Vanuatu, and 595.2/.7
- Language:
- English
- Description:
- Four species new to science are described from Vanuatu, namely Holophygdon nishidae sp. n., Idiocysta vanuatuensis sp.n., Nesocypselas minicysta sp. n., and Tingis parvoroe sp. n. New records are provided for six species, five of them being new to Vanuatu: Cysteochila idonea Drake, Stephanitis subfasciata Horváth, Teleonemia scrupulosa Stål, Tingis chazeaui Guilbert, and Tingis irregularis (Montrouzier). A key to genera and species of Vanuatu is added., Eric Guilbert, and Lit
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public