Ten polymorphic microsatellite loci were developed for the butterfly Erebia palarica (Nymphalidae, Satyrinae), endemic to NW Spain. Polymorphism for 20 individuals from a NW Spanish population (Ancares) and 15 more from two other nearby localities (Courel, Trevinca) was assessed. Overall, the number of alleles per locus ranged from six to 24. Ancares showed an average number of alleles per locus of 14.30 (SD = 5.32), observed heterozygosity of 0.753 (SD = 0.14) and unbiased expected heterozygosity of 0.818 (SD = 0.114). Genotypic frequencies conformed to the Hardy-Weinberg equilibrium at Ancares, and there was no evidence of linkage disequilibrium. Multilocus genotypes resulting from this set of markers will be useful for determining genetic diversity and differentiation within and among populations of this local endemic butterfly in NW Spain. Of the six loci amplified in the closely related species, Erebia meolans, five were polymorphic.
In this study, we report the development of a set of 15 polymorphic microsatellite markers for the box tree moth, Cydalima perspectalis (Walker), a highly invasive insect in Europe causing significant damage to natural and ornamental Buxus trees. The markers were characterized for four distant populations in both its native (China, two populations) and invasive ranges (Czech Republic and Turkey, one population each). The number of alleles ranged from 2 to 12. No marker significantly deviated from the Hardy-Weinberg equilibrium for all the populations sampled. These microsatellite markers are promising tools for further studies on the invasive pathways and dispersal pattern of the box tree moth in Europe., Audrey Bras, Laure Sauné, Alain Roques, Jérôme Rousselet, Marie-Anne Auger-Rozenberg., and Obsahuje bibliografii