Mean development rates under cycling temperature regimes (both alternating and sinusoidal regimes) have been found to be either accelerated, decelerated or unaffected when compared to development at constant temperature regimes with equivalent means. It is generally accepted that this phenomenon is a consequence of the non-linearity inherent in the temperature-rate relationship of insect development and is known as the rate summation, or Kaufmann, effect. Some researchers invoke an additional physiological mechanism or specific adaptation to cycling temperatures resulting in a genuine alteration of development rate. Differences in development rates at constant and cycling temperatures may have important implications for degree-day (linear) population models, which are used in bath pest management and ecological studies.
Larvae of Aglais urticae L. (small tortoiseshell), Inachis io L. (peacock), Polygonia c-album L. (comma) and Vanessa atalanta L. (red admiral) (Lepidoptera: Nymphalidae) were reared at constant (10, 15, 20, 25, 30°C) and alternating (20/10, 25/15, 30/10, 30/20°C) regimes. Development rates under the alternating regimes used were found to differ from those under equivalent constant temperatures in a pattern suggestive of the Kaufmann effect: in all species development at 20/10°C was faster than at 15°C, and for three species development at 30/20°C was slower than at 25°C. The exception was A. urticae. A similar pattern was found for growth rate and pupal weight. The results are discussed with respect to cycling temperature theory and degree-day modelling., Simon R. Bryant, Jeffrey S. Bale, Chris D. Thomas, and Lit
The presence of adipokinetic activity in crude extracts of corpora cardiaca (CC) from the butterfly (Vanessa cardui L., Nymphalidae) was demonstrated by bioassay and Mas-AKH was revealed as the major adipokinetic hormone (AKH) by use of two different technologies of sequence elucidation: HPLC separation of the peptide followed by Edman degradation and Q-TOF mass spectrometry. In contrast to the time- and material-consuming conventional methods of peptide purification and sequencing, substantial structural data of the peptide were confirmed - post factum - from one pCC (pair of CC) by Q-TOF mass spectrometry. Only males of our laboratory colony showed a significant lipid increase in the haemolymph after injection of either crude CC extract (1 pCC equivalent) or 10 pmol of synthetic peptide., Gabriele Köllisch, Peter D. Verhaert, Matthias W. Lorenz, Roland Kellner, Gerd Gäde, Klaus H. Hoffmann, and Lit