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2. Effect of Zn2+ stress in rice (Oryza sativa cv. Manhar) on growth and photosynthetic processes
- Creator:
- Ajay and Rathore, V.S.
- Format:
- Type:
- model:internalpart and TEXT
- Subject:
- carotenoids, thylakoids, sugars, starch, relative growth rate, photosynthates, Hill reaction, cyclic and non-cyclic photophosphorylation, and chlorophyll
- Language:
- Multiple languages
- Description:
- Effect of three Zn2+ concentrations, i.e. 0.075 (cl), 7.50 (c2) and 37.5 (c3) jiM, on rice seedlings was studied at three stages, i.e. 1, 14 and 21 d after transplantation. Typical deficiency symptoms were observed in both solution and sand cultures of cl and c2, but the effects were more pronounced in the solution culture. The c3 concentration was toxic. There was marked reduction in growth, chlorophyll (Chl) contents (particularly Chl b), Hill reaction activity, photophosphorylation rate (particularly non-cyclic photophosphorylation), thylakoid phosphorylation, and i'‘C02-fixation at the cl concentration. However, a similar reduction was also observed in thylakoid phosphorylation at the c3 concentration. Hence the optimum zinc concentration in the nutrient medium lied between c2 and c3 Zn2+. By regression the theoretical optimum Zn concentration was calculated as 19.20 pM (1.28 mg kg'i) Zn2+. Partitioning of ^'^C-photosynthates indicated reduced allocation to sugar and starch fractions and increased fřee amino acids concentration at the cl concentration and vice-versa at c2 and c3.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3. Influence of exogenously applied 6-benzylaininopunne on the structure of chloroplasts and arrangement of their membranes
- Creator:
- Wilhelmová, N. and Kutík, J.
- Format:
- Type:
- model:internalpart and TEXT
- Subject:
- carotenoids, thylakoid stacking, starch inclusions, proteins, Nicotiana tabacum\ plastoglobuli, electron microscopy, digitonin fractionation, cytokinin, and chlorophyll
- Language:
- Multiple languages
- Description:
- The influence of a cytokinin, 6-benzylaminopurine (BAP), on chloroplast structure was studied using biochemical methods and electron microscopy. The average degree of thylakoid stacking was determined by digitonin fractionation and differential centrifugation of chloroplasts from tobacco plantlets after treatment with different concentrations of BAP in agar medium during cultivation in viíro. An elevated concentration of BAP in the medium induced an increase in grana stacking. This was in accordance with the lowering of the chlorophyll a/b ratio in these chloroplasts. The relative amoímt of proteins and carotenoids increased in both stromal and (to a lesser extent) granal chloroplast thylakoid fractions with the BAP concentration. The electron microscopic studies revealed nearly the same volume density of thylakoid membranes within chloroplasts of BAP treated plantlets and control ones. In the BAP treated plantlets the chloroplasts were smaller and had a profound accumulation of starch inclusions and a more flattened shape than the chloroplasts of control plantlets. The volume density of plastoglobuli in chloroplasts did not decrease under the influence of BAP,
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public