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2. Effect of chlorophyll-protein complex I deficiency on the physiological character of a Chlamydomonas reinhardtii mutant
- Creator:
- Tang, Qing-Xiu, Ni, Zhang-Lin, and Wei, Jia-Mian
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- ATPase, electron transport, NADP, cyclic and non-cyclic photophosphorylation, photosystems 1 and 2, and proteins
- Language:
- Multiple languages
- Description:
- In the mutant CC-1047 of Chlamydomonas reinhardtii, LDS-PAGE showed that the chlorophyll-protein complex I (CPI) is almost absent. The mutant could not grow in a culture medium without organic carbon source while the wild type (WT) C. reinhardtii grew quickly. When an organic carbon source was added into the culture medium, the mutant grew almost as well as WT. The rate of photosystem 1 (PS1) electron transport (DCPIP→MV) and the rate of whole chain electron transport (H2O→MV) of chloroplasts of the CC-1047 mutant were both lower than those of WT. The photophosphorylation activity, photosynthetic O2 evolution rate, and rate of NADP+ photoreduction of CC-1047 were also much lower than the activities of WT. There were some differences in ATPase activity between the mutant and WT. Two different activation ways were used to activate the latent ATPase using methanol and dithiothreitol (DTT) as activation substrate. More methanol and DTT were required for the mutant than WT to obtain the maximum activity. Thus the photosynthetic apparatus could not operate normally when CPI was absent because of the abnormal PS1 electron transport. Meanwhile, the other adjacent complexes of the thylakoid membrane, for example, ATP synthase complex, were slightly affected. and Qing-Xiu Tang, Zhang-Lin Ni, Jia-Mian Wei.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3. Effect of iron deficiency induced changes on photosynthetic pigments, ribulose-1,5-bisphosphate carboxylase, and photosystem activities in field grown grapevine (Vitis Vinifera L. cv. Pinot noir) leaves
- Creator:
- Bertamini, M., Nedunchezhian, N., and Borghi, B.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- carotenoids, chlorophyll, fluorescence, electron transport, photosystems 1 and 2, proteins, and ribulose-1,5-bisphosphate carboxylase
- Language:
- Multiple languages
- Description:
- The effect of iron deficiency on photosynthetic pigments, ribulose-1,5-bisphosphate carboxylase (RuBPC), and photosystem activities were investigated in field grown grapevine (Vitis vinifera L. cv. Pinot noir) leaves. The contents of chlorophyll (Chl) (a+b) and carotenoids per unit fresh mass showed a progressive decrease upon increase in iron deficiency. Similar results were also observed in content of total soluble proteins and RuBPC activity. The marked loss of large (55 kDa) and small (15 kDa) subunits of RuBPC was also observed in severely chlorotic leaves. However, when various photosynthetic electron transport activities were analysed in isolated thylakoids, a major decrease in the rate of whole chain (H2O → methyl viologen) electron transport was observed in iron deficient leaves. Such reduction was mainly due to the loss of photosystem 2 (PS2) activity. The same results were obtained when Fv/Fm was evaluated by Chl fluorescence measurements in leaves. Smaller inhibition of photosystem 1 (PS1) activity was also observed in both mild and severely chlorotic leaves. The artificial electron donors, diphenyl carbazide and NH2OH, markedly restored the loss of PS2 activity in severely chlorotic leaves. The marked loss of PS2 activity was evidently due to the loss of 33, 23, 28-25, and 17 kDa polypeptides in iron deficient leaves. and M. Bertamini, N. Nedunchezhian, B. Borghi.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
4. Photoinhibition and recovery of photosystem 2 in grapevine (Vitis vinifera L.) leaves grown under field conditions
- Creator:
- Bertamini, M. and Nedunchezhian, N.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- chlorophyll fluorescence, diurnal course, donor side, electron transport, photosystems 1 and 2, and proteins
- Language:
- Multiple languages
- Description:
- Photoinhibition of photosynthesis was investigated in grapevine (Vitis vinifera L.) exposed to 2 or 4h of high irradiance (HI) (1 700-1 800 μmol m-2 s-1) leaves under field conditions at different sampling time in a day. The degree of photoinhibition was determined by means of the ratio of variable to maximum chlorophyll fluorescence (Fv/Fm) and photosynthetic electron transport measurements. When the photochemical efficiency of photosystem 2 (PS2), Fv/Fm, markedly declined, F0 increased in both 2 (HI2) and 4 h (HI4) HI leaves sampled at midday. When various photosynthetic activities were followed on isolated thylakoids, HI4 leaves showed significantly higher inhibition of whole chain and PS2 activity than the HI2 leaves sampled at midday. Later, the leaves reached maximum PS2 efficiencies similar to those observed early in the morning during sampling at evening. The artificial exogenous electron donor Mn2+ failed to restore PS2 activity in both variants of leaves, while DPC and NH2OH significantly restored PS2 activity in HI4 midday leaf samples. Quantification of the PS2 reaction centre protein D1 and 33 kDa protein of water splitting complex following midday exposure of leaves showed pronounced differences between HI2 and HI4 leaves. The marked loss of PS2 activity noticed in midday samples was mainly due to the marked loss of D1 protein in HI2, while in HI4 it was mainly 33-kDa protein. and M. Bertamini, N. Nedunchezhian.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
5. Photoinhibition of photosynthesis in leaves of grapevine (Vitis vinifera L. cv. Riesling). Effect of chilling nights
- Creator:
- Bertamini, M., Muthuchelian, K., Rubinigg, M., Zorer, R., and Nedunchezhian, N.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- acceptor side, chlorophyll fluorescence, D1 protein, diurnal course of photosynthetic activities, electron transport, Mn, NH2OH, photosystems 1 and 2, and photoinhibition
- Language:
- Multiple languages
- Description:
- Photoinhibition of photosynthesis was investigated in control (C) and chilling night (CN) leaves of grapevine under natural photoperiod at different sampling time in a day. The degree of photoinhibition was determined by means of the ratio of variable to maximum chlorophyll fluorescence (Fv/Fm) and photosynthetic electron transport measurements. When the potential efficiency of photosystem (PS) 2, Fv/Fm was measured at midday, it markedly declined with significant increase of F0 in CN leaves. In isolated thylakoids, the rate of whole chain and PS2 activity were markedly decreased in CN leaves than control leaves at midday. A smaller inhibition of PS1 activity was also observed in both leaf types. Later, the leaves reached maximum PS2 efficiencies similar to those observed in the morning during sampling at evening. The artificial exogenous electron donors diphenyl carbazide, NH2OH, and Mn2+ failed to restore the PS2 activity in both leaf types at midday. Thus CN enhanced inactivation on the acceptor side of PS2 in grapevine leaves. Quantification of the PS2 reaction centre protein D1 following midday exposure of leaves showed pronounced differences between C and CN leaves. The marked loss of PS2 activity in CN leaves noticed in midday samples was mainly due to the marked loss of D1 protein of the PS2 reaction centre. and M. Bertamini ... [et al.].
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
6. Photoinhibition of photosynthesis in sun and shade grown leaves of grapevine (Vitis vinifera L.)
- Creator:
- Bertamini, M., Muthuchelian, K. , and Nedunchezhian, N.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- chlorophyll fluorescence, donor side, D1 protein, electron transport, photoinhibition, and photosystems 1 and 2
- Language:
- Multiple languages
- Description:
- The degree of photoinhibition of sun and shade grown leaves of grapevine was determined by means of the ratio of variable to maximum chlorophyll (Chl) fluorescence (Fv/Fm) and electron transport measurements. The potential efficiency of photosystem 2 (PS2), Fv/Fm, markedly declined under high irradiance (HI) in shade leaves with less than 10 % of F0 level. In contrast, Fv/Fm ratio declined with about 20 % increase of F0 level in sun leaves. In isolated thylakoids, the rate of whole chain and PS2 activity in HI shade and sun leaves was decreased by about 60 and 40 %, respectively. A smaller inhibition of photosystem 1 (PS1) activity was also observed in both leaf types. In the subsequent dark incubation, fast recovery was observed in both leaf types that reached maximum PS2 efficiencies similar to non-photoinhibited control leaves. The artificial exogenous electron donors DPC, NH2OH, and Mn2+ failed to restore the HI-induced loss of PS2 activity in sun leaves, while DPC and NH2OH were significantly restored in shade leaves. Hence HI in shade leaves inactivates on the donor side of PS2 whereas it does at the acceptor side in sun leaves, respectively. Quantification of the PS2 reaction centre protein D1 and the 33 kDa protein of water splitting complex following HI-treatment of leaves showed pronounced differences between shade and sun leaves. The marked loss of PS2 activity in HI leaves was due to the marked loss of D1 protein of the PS2 reaction centre protein and the 33 kDa protein of the water splitting complex in sun and shade leaves, respectively. and M. Bertamini, K. Muthuchelian, N. Nedunchezhian.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
7. Protective effect of triacontanol against acidic mists in Samanea saman (Jacq.) Merrill seedlings: Differential responses in growth, 14CO2 fixation, ribulose-1,5-bisphosphate carboxylase, and electron transport activities
- Creator:
- Muthuchelian, K., Meenakshi, V., and Nedunchezhian, N.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- cow tamarind, donor side, electron transport, fresh and dry matter, leaf density and area, nitrate reductase, photosystems 1 and 2, and root and shoot length
- Language:
- Multiple languages
- Description:
- Seedlings of tropical leguminous tree Samanea saman (Jacq.) Merrill were exposed for 7 d to acidic mist (AM, induced by H2SO4) of pH 5.6, 4.0, and 2.0. AM significantly reduced seedling growth (root and shoot length, leaf density, leaf area, fresh and dry mass accumulation) and photosynthetic activities. In thylakoids isolated from leaves treated at pH 4.0 and 2.0 a decrease in the activities of photosystem (PS) 2 and whole chain electron transport was observed, but PS1 activity did not change. When the seedlings were subsequently sprayed with triacontanol (TRIA), the AM effect was partially or completely reversed indicating that TRIA can protect from AM effects. The artificial electron donors, di-phenylcarbazide (DPC) and hydroxylamine (NH2OH), markedly restored the loss of PS2 activity in AM (pH 2.0) treated leaves. This is the first report of alleviating the AM by TRIA in tropical tree seedlings. and K. Muthuchelian, V. Meenakshi, N. Nedunchezhian.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
8. Shade effect alters leaf pigments and photosynthetic responses in Norway spruce (Picea abies L.) grown under field conditions
- Creator:
- Bertamini, M., Muthuchelian, K., and Nedunchezhian, N.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- chlorophyll fluorescence, diphenyl carbazide, donor side, electron transport, NH2OH, photosystems 1 and 2, polypeptides, and ribulose-1,5-bisphosphate carboxylase
- Language:
- Multiple languages
- Description:
- The contents of chlorophyll (Chl) and carotenoids (Car) per fresh mass were lower in shade needles than in sun needles. Ribulose-1,5-bisphosphate carboxylase (RuBPC) activity and contents of soluble proteins were also significantly lower in shade needles. In isolated thylakoids, a marked lower rate of whole chain and photosystem (PS) 2 activities were observed in shade needles. Smaller lower rate of PS1 activity was also observed in shade needles. The artificial exogenous electron donors, diphenyl carbazide (DPC) and NH2OH, significantly restored the loss of PS2 activity in shade needles. Similar results were obtained when Fv/Fm was evaluated by Chl fluorescence measurements. The marked lower rate of PS2 activity in shade needles was due to the lower contents of 47, 33, 28-25, 23, and 17 kDa polypeptides. This conclusion was confirmed by immunological studies showing that the content of the 33 kDa protein of the watersplitting complex was diminished significantly in shade needles. and M. Bertamini, K. Muthuchelian, N. Nedunchezhian.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public