Changes in pigment composition and chlorophyll (Chl) fluorescence parameters were studied in 20 year-old Scots pine (Pinus sylvestris L.) trees grown in environment-controlled chambers and subjected to ambient conditions (CON), doubled ambient CO2 concentration (EC), elevated temperature (ambient +2-6 °C, ET), or a combination of EC and ET (ECT) for four years. EC did not significantly alter the optimal photochemical efficiency of photosystem 2 (PS2; Fv/Fm), or Chl a+b content during the main growth season (days 150-240) but it reduced Fv/Fm and the Chl a+b content and increased the ratio of total carotenoids to Chl a+b during the 'off season'. By contrast, ET significantly enhanced the efficiency of PS2 in terms of increases in Fv/Fm and Chl a+b content throughout the year, but with more pronounced enhancement in the 'off season'. The reduction in Fv/Fm during autumn could be associated with the CO2-induced earlier yellowing of the leaves, whereas the temperature-stimulated increase in the photochemical efficiency of PS2 during the 'off season' could be attributed to the maintenance of a high sink capacity. The pigment and fluorescence responses in the case of ECT showed a similar pattern to that for ET, implying the importance of the temperature factor in future climate changes in the boreal zone. and K. Y. Wang, S. Kellomäki, T. Zha.