This study examines quality of academic worklife in Czech public universities to assess the extent to which the global drive towards marketisation in higher education has affected Czech academic staff. A total of 2229 academics (men = 57.1%) completed a survey measuring their job satisfaction, job stress, and work environment perceptions. Findings revealed high levels of overall job satisfaction (83.6% satisfied with their jobs) and relatively low levels of stress (13.7% regularly stressed). Most academics reported positive features of their work environment including autonomy and quality, role clarity, influence over academic work, and a strong social community. Negative features included dissatisfaction with pay, poor leadership, and pressure to produce. Job satisfaction was significantly associated with traditional academic values (focus on quality, involvement in decision-making, commitment to the workplace, recognition), while stress was linked to market-related aspects (pressure to produce, quantitative work demands, job insecurity). The study highlighted relatively high levels of well-being among Czech faculty, which can be attributed to the continued prevalence of a traditional, professor-oriented academic system based on autonomy and collegiality. Despite recent market-oriented changes within Czech research policy, the negative effects of marketisation are not yet pronounced in the quality of academic worklife in public universities, except for the increasing pressure for productivity.