The fresh weight, dry weight, and C and N content of the eggs, egg shells and neonate larvae of several satyrines were measured. This was done in order to assess the specificity of the composition of the egg and larvae, the phylogenetic or ecological nature of the variation and the existence of structural constraints on the composition of the offspring. All the traits investigated were found to be highly species-specific. The nature of the variation was not primarily phylogenetic, suggesting that the composition of the offspring has an ecological meaning. However, only a slight association was detected between three life history traits or habitat features and the compositions of the eggs or larvae, namely: female egg dropping was associated with a high C content of the eggs, xerophily with a high C : N ratio, and a high content of N in the larvae with egg diapause. The evidence for intra-specific allometry between the traits investigated and egg weight varied among the species, suggesting that the slope of such relationship may be a specific feature. There was a close to isometric relationship between C and N contents in every species. Therefore simple C : N ratios are independent of egg size, hence they can be used directly in comparative studies. Across species analyses indicated that small offspring contained a proportionally low amount of carbon and had a high dry matter content, suggesting that selection for small eggs was accompanied by selection for an enhanced proportion of nitrogen per egg. Finally, the species with large adult females invested comparatively more nitrogen per egg, which indicates a potential, constraint-based advantage of large adult size.
Although most monitoring protocols characterize fish assemblages based on one sampling occasion per year per site, it is largely unknown how well such snapshot samples characterize fish assemblages at the site and the stream levels. To address these issues, we conducted monthly samplings from March to November in 2009 in two wadeable lowland streams in the catchment area of Lake Balaton, Hungary. Five and seven sites were investigated in the two streams by electric fishing 150 m long sections. For a given sampling site, mean estimated species composition of a single survey showed on average 41 % and 35 % Jaccard index based similarity to the pooled annual samples of the site, and 90 % species representation could be reached using 5.2 and 6.4 sampling occasions on average. The representativeness of relative abundance data also varied considerably in time, showing on average 51 % and 67 % Bray-Curtis index based similarity to the pooled annual samples of the site, and reached 90 % similarity by taking 4.2 and 5.4 surveys on average per year per site. Stream level simulations of sample representativeness showed that a single survey reached on average 62.3 % and 66 % Jaccard similarity and 75.7 % and 74.8 % Bray-Curtis similarity to the whole year dataset. At the stream level, 90 % representativeness of both species composition data and relative abundance data was reached by pooling four surveys for both streams. These results indicate considerable within year variability in lowland stream fish assemblages, which should not be forgotten when evaluating monitoring data, which are based on a single survey per year.
Wnt/β-catenin signaling is involved in virtually every aspect of embryonic development and also controls homeostatic selfrenewal in a number of adult tissues. Recently, emerging evidence from researches of organ fibrosis suggest that sustained Wnt/β-catenin pathway reactivation is linked to the pathogenesis of fibrotic disorders. Here we focus on Wnt/β-catenin-related pathogenic effects in different organs, such as lung fibrosis, liver fibrosis, skin fibrosis and renal fibrosis. Additionally, Wnt/β- catenin signaling works in a combinatorial manner with TGF-β signaling in the process of fibrosis, and TGF-β signaling can induce expression of Wnt/β-catenin superfamily members and vice versa. Moreover, network analysis, based on pathway databases, revealed that key factors in the Wnt pathway were targeted by some differentially expressed microRNAs detected in fibrosis diseases. These findings demonstrated the crosstalks between Wnt/β-catenin pathway and TGF-β signalings, and microRNAs, highlighting the role of Wnts in organ fibrogenesis. Most importantly, nowadays there is a variety of Wnt pathway inhibitors which give us the potential therapeutic feasibility, modulation of the Wnt pathway may, therefore, present as a suitable and promising therapeutic strategy in the future., Y. Guo ... [et al.]., and Obsahuje seznam literatury
Wolbachia is a maternally transmitted intracellular symbiont which causes reproductive distortions in the arthropods it infects. In recent years there has been an increasing interest in using Wolbachia as a potential tool for biological control by genetic manipulation of insect pests. In the present paper we report Wolbachia infection in several Trissolcus wasps (Hymenoptera: Scelionidae) which are important egg parasitoids of the sunn pest, Eurygaster integriceps Puton (Heteroptera: Scutellaridae). We used DNA sequence data for a gene encoding a surface protein of Wolbachia (wsp) not only to confirm Wolbachia infection but also to discriminate Wolbachia strains. Phylogenetic analyses indicated that Wolbachia strains in Trissolcus species were closely related to one another and belonged to supergroup B. Determination of the infection status of various populations, the possible role of Wolbachia in causing the incompatibility and knowledge of the reproductive compatibility of Trissolcus populations is important for the success of parasitoids in sunn pest management., Nurper Guz ... [et al.]., and Obsahuje seznam literatury
Wolbachia pipientis (Hertig) (Rickettsiaceae) is an endocellular bacterium infecting numerous species of arthropods. The bacterium is harboured by males and females but is only transmitted maternally because spermatocytes shed their Wolbachia during maturation. The presence of this endosymbiont can lead to feminisation of the host, parthenogenesis, male-killing or reproductive incompatibility called cytoplasmic incompatibility (CI). Although Wolbachia transmission is exclusively maternal, phylogenetic evidence indicates that very rare inter-species transmission events have taken place. Horizontal transmission is possible in the laboratory by transferring cytoplasm from infected to uninfected eggs. Using this technique, we have artificially infected lines of the fruit fly Drosophila simulans Sturtevant (Drosophilidae). Recipient lines came from two different D. simulans populations. One ("naive" host) is not infected in the wild. The other ("usual" host) is a population naturally carrying Wolbachia in the wild. In this second case, recipient flies used in the experiment came from a stock culture that had been cured off its infection beforehand by an antibiotic treatment. Infected D. simulans laboratory stocks were used as donors. We assessed the three following parameters: (i) trans-infection success rate (ratio of infected over total female zygote having survived the injection), (ii) level of cytoplasmic incompatibility expressed by trans-infected males three generations post-trans-infection, and (iii) infection loss rate over time in trans-infected lines (percentage of lines having lost the infection after 20 to 40 generations). We observed that parameter (i) did not differ significantly whether the recipient line came from a "naive" or a "usual" host population. However, both (ii) and (iii) were significantly higher in the "naive" trans-infected stock, which is in agreement with earlier theoretical considerations.