In order to investigate the ability of infective larvae of the nematode Baylisascaris transfuga (Rudolphi, 1819) Sprent, 1968 to hatch from the egg-shells and then to migrate in tissues, parenteral infections of mice with embryonated eggs were performed. Two groups of outbred albino mice were infected with approximately 3500 B. transfuga infective eggs sub-cutaneously (SC) or intraperitoneally (IP). B. transfuga larvae in the IP group rapidly hatched and migrated to the intestine, liver, lungs, brain and carcass. Subcutaneous inoculation of eggs was followed also by migration of hatched larvae in the examined organs. In the SC mice, extensive encapsulating reactions involving the subcutaneous tissues and carcass, and containing large numbers of hatched eggs and free motile larvae, were found at the sites of inoculation. Some differences in the migratory behaviour were observed between the two groups. It is shown that B. transfuga infective larvae are able to hatch and migrate in tissues of mice, and tend to settle and/or to be trapped in the intestinal wall and muscles, even after parenteral inoculations of embryonated eggs. These results could provide basic data for further investigations on the migratory pathways of B. transfuga larvae or to perform immunological and therapeutical studies.
Photosynthetic assimilatíon of CO2 in a four-year-old plant of lilac, measured in April and in July, was compared. The results were calculated with regard to the surface area of the particular year groups of the stems and to the total surface area of the stems as well as to the globál surface area of the leaves of the plant. In April the stems were the only site of photosynthesis. In July the main organs of CO2 assimilatíon were the leaves, while the participation of the shoots in that period amounted to 2 %. In the process of photosynthesis in the stems mainly the endogenous CO2 was utilized, while the share of exogenous CO2 was 0.02 %. The potential photosynthesis was determined also on the basis of measurements of oxygen release by chloroplasts isolated from the bark and leaves. In July the production of oxygen by chloroplasts ffom the bark of all stems was 5 % of the amount of oxygen released by the chloroplasts isolated from the leaves. In April the production of oxygen by chloroplasts isolated from the bark of the particular year groups of the stems was higher than in July. In the process of CO2 assimilatíon by the bark and leaves the potential Chemical activity of chloroplasts was not fully utilized. The potential CO2 assimilatíon by chloroplasts isolated from the bark was 8.5 times greater than the measured results of CO2 exchange in July and 35.8 times greater in April.
The pastoral blowing of Christmas Eve belongs to the most remarkable Christmas traditions in Bohemian lands. The pastoral tuba (tuba pastoritia) was made by the shepherds themselves from wood, later it was made in a craftsman manner from brass. The records of the time corroborate the outstanding musical skills of some shepherds. Their signals on the pasture followed up with military or fireman tunes. On Christmas Eve the shepherds and night watchmen made walk-abouts with singing and blowing. In the frontier regions the costume of rounding up the sheep to Bethlehem had been preserved. The shepherd cracked the whip and blew the tuba and before him were running boys with collars and bells. Still at the end of the twentieth century Josef Svejkovský recorded in the regions of Rokycany and Zbiroh remarkable pastoral tunes that were played on the pasture as well as on Christmas Eve. A unique chapter of the pastoral blowing represents its use in church during Christmas ceremonies. Pastorales composed by Bohemian and Moravian schoolmasters required the pastoral tuba and later the trumpet. Some parts indicate a virtuoso artistic production of the interpreters. The tuba pastoralis had been used in two ways, either during the whole composition or later only in the overture and in the intermezzos, rather as a curiosity. This popular instrument entered the festival Christmas intrades and also the instrumental pastorales. Unique „Symphonia pastoralis“ from Dřevohostice (1788) is a small concerto grosso with solo violin and tuba pastoralis. On midnight mess sometimes two shepherds blew, each of them in a different part of the church. The chordal motives that are characteristic for the pastoral tuba were often imitated in pastorales for other instruments, usually for violines and also pastoral organ preludes. Rare are pastorales for cemballo that in the eighteenth century probably served as a home Christmas music.