Hyperbaric oxygen preconditioning (HBO-PC) has been proposed as a safe and practical approach for neuroprotection in ischemic stroke. However, it is not known whether HPO-PC can improve cognitive deficits induced by cerebral ischemia, and the mechanistic basis for any beneficial effects remains unclear. We addressed this in the present study using rats subjected to middle cerebral artery occlusion (MCAO) as an ischemic stroke model following HBO-PC. Cognitive function and expression of phosphorylated neurofilament heavy polypeptide (pNF-H) and doublecortin (DCX) in the hippocampus were evaluated 14 days after reperfusion and after short interfering RNA-mediated knockdown of sirtuin1 (Sirt1 ). HBO-PC increased pNF-H and DCX expression and mitigated cognitive deficits in MCAO rats. However, these effects were abolished by Sirt1 knockdown. Our
results suggest that HBO-PC can protect the brain from injury caused by ischemia-reperfusion and that Sirt1 is a potential molecular target for therapeutic approaches designed to minimize cognitive deficits caused by cerebral ischemia.
Sirtuin 1 (SIRT1) is involved in important biological processes such as energy metabolism and regulatory functions of the cell cycle, apoptosis, and inflammation. Our previous studies have shown hepatoprotective effect of polyphenolic compound resveratrol, which is also an activator of SIRT1. Therefore, the aim of our present study was to clarify the role of SIRT1 in process of hepatoprotection in animal model of drug-induced liver damage. Male Wistar rats were used for both in vivo and in vitro studies. Hepatotoxicity was induced by single dose of acetaminophen (APAP). Some rats and hepatocytes were treated by resveratrol or synthetic selective activator of sirtuin 1 (CAY10591). The degree of hepatotoxicity, the activity and expression of the SIRT1 were determined by biochemical, histological and molecular-biological assessments of gained samples (plasma, liver tissue, culture media and hepatocytes). Resveratrol and CAY attenuated APAP-induced hepatotoxicity in vivo and in vitro. Moreover, both drugs enhanced APAPreduced SIRT1 activity. Our results show that modulation of the SIRT1 activity plays a role in hepatoprotection. Synthetic activators of SIRT1 would help in understanding the role of SIRT1 and are therefore a major boost towards the search for specific treatment of liver disease., L. Wojnarová, N. Kutinová Canová, H. Farghali, T. Kučera., and Obsahuje bibliografii
In its judgment T-341/07 Sison III, the General Court made the conditions of EU non-contractual liability in relation to the so-called smart sanctions even stricter that under ordinary circumstances. It construed the condition of sufficient seriousness of a breach by EU institutions in a way that even in cases with no room for discretion this lability does not occur quasi-automatically but only if the decision-making institution fails to act as “an administrative authority, exercising ordinary care and diligence”: such administration may be– due to the circumstances – even wrong. Thus the General Court indicates that for the EU is not so difficult to admit violation of fundamental rights on the EU side, but the problem is to admit its obligation to pay damages. The article also examines the question whether it is in line with EU constitutional principles to have a different set of conditions for EU a its Member States’ extra-contractual liabilaty and a different set for individuals.
Myrmica rubra is a northern, temperate Palaearctic ant species with a geographical range that extends from the Atlantic coast of Europe to central Asia. In Europe, its range covers > 25° of latitude where it lives under a variety of climates that vary from extreme oceanic in the west, to continental in the east. Colonies nest in the soil and their life cycles are known to be highly dependent on ambient temperature and soil moisture. We hypothesised that the brood-rearing behaviour of populations might be focally adapted to climate and that we might detect differences when the ants were reared under \"common-garden\" conditions. Brood-rearing behaviour was compared for 38 colonies of M. rubra drawn from 13 populations representing a range of 6 latitudes: all 6 were represented in eastern Europe and 2 in western Europe. A sample of ants from each colony was used to estimate respiration rate, body mass and fat content at the start of the experiment in spring (immediately post hibernation) and at the end of the experiment (mid summer). Respiration had a linear relationship with latitude, with northern populations having greater respiration rate in spring compared to southern populations. It is suggested that this is an adaptation to different seasonality over the species' range that results in the \"more active\" northern workers rearing fewer brood to maturity more quickly than southern workers. Fat content, a measure of worker \"quality\", had a parabolic relationship with latitude with mid latitude colonies having the fattest workers. Fatter workers appeared to rear heavier brood. This probably represented a functional response to environment with populations living at \"edge of range\" sites being physiologically more stressed and performing brood-rearing tasks less well than centre of range populations. We believe that this is the first demonstration of a consistent, intra-specific trend for Variation in the social physiology of an ant species over its geographic range., Graham W. Elmes, Judith C. Wardlaw, Mogens G. Nielsen, Vladilen E. Kipyatko, Elena B. Lopatina, Alexander G. Radchenko, Boyd Barr, and Lit
The authors analyse the discourse of Green Ways (GW), a company using multi-level marketing where women comprise the majority of distributors. The article shows that however multi-level marketing is advertised as a highly flexible form of employment suitable for those who want to combine family life with work, it is rather a way of marketing than an employment opportunity. A significant role in this business is played by women on parental leave who earn self-esteem based in the neoliberal values of self-reliance and entrepreneurial success, rather than FINANCIAL income. The analysis links their ways of describing the character and benefits of selling GW products with the ideology presented in GW manuals for distributors. Using Bourdieu’s theory, the authors point out how GW constructs the symbolic oppositional binary in line with the neoliberal notion of an efficient individual., Irena Lištiaková, Lucie Jarkovská., and Obsahuje seznam literatury