The life cycle of marine Eubothrium sp. (Cestoda: Pseudophyllidea), from Atlantic salmon (Salmo salar L.) was experimentally completed in one year and included only one intermediate host (Acartia tonsa Dana) (Copepoda: Calanoida). Adult cestodes were collected from farmed salmon, and ripe eggs released by the cestodes were fed to Acartia tonsa. Ingested eggs hatched in the gut and the larvae developed in the haemocoel of the copepod for 15 days at 16°C. A total of 170 seawater-reared salmon were exposed to infected copepods and the total prevalence of Eubothrium sp. in the salmon after infection was 95.3%, with a mean intensity of 15.0 (range 1-87). The infected salmon were kept in the laboratory where the growth of the cestodes was studied for eleven months. Mean length of the cestodes increased with time, but a large variation among the cestodes was observed. Growth and maturation of the cestodes were dependent on host size and the number of worms present in the intestine. No evidence of mortality of Eubothrium sp. was observed during the experimental period.
An experimental infection with the microsporidian Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923 was studied using a model of immunocompetent BALB/c mice and immunodeficient SCID mice. The course of infection after intraperitoneal inoculation of E. cuniculi spores was evaluated using the presence of spores in peritoneal macrophages as a criterion. First significant decrease in the proportion of infected cells was recorded on day 9 post infection (p.i.) in BALB/c mice. From day 14 p.i. no spores were observed in macrophages from BALB/c mice, while the number of infected macrophages from SCID mice increased until the death of the mice. The natural killer (NK) cell activity of mouse splenocytes was compared with the production of interferon gamma (IFN-γ) by these cells. While in BALB/c mice NK activity peaked on days 9 and 14 p.i., in SCID mice the marked increase of NK activity was recorded close before death of mice, on day 21 p.i. in correlation with the production of IFN-γ. Production of specific antibodies was demonstrated from day 9 p.i. in sera from BALB/c mice. It is concluded that intraperitoneal infection of SCID mice with spores of E. cuniculi results in the marked increase in the number of peritoneal exudate cells and in the percentage of infected cells close before death of mice. Neither high activity of NK cells nor increased production of IFN-γ are sufficient for the recovery of SCID mice from an E. cuniculi infection.
In the paper, the authors refer to measurement of a car cabin heat load and indoor environment during driving in real traffic conditions. The primary aim was to obtain data for boundary conditions, model testing and results comparison of the 1D car cabin heat load model. The secondary aim was better understanding of cabin environment inside a car cabin under different operational conditions. As test vehicle Škoda Felicia Combi with dark blue painting was used and GPS data, parameters of ventilation, cabin environnment and ambient environment were measured. Measurements were preformed for summer, autumn and winter conditions including parking and driving test circuits around Brno. Driving circuit included driving in the city, out of the city and on the highway D1. and Obsahuje seznam literatury
The effect of solid concentration and mixture velocity on the flow behaviour, pressure drops, and concentration distribution of coarse particle-water mixtures in horizontal, vertical, and inclined smooth stainless steel pipes of inner diameter D = 100 mm was experimentally investigated. Graded basalt pebbles were used as solid particles. The study revealed that the coarse-grained particle-water mixtures in the horizontal and inclined pipes were significantly stratified. The solid particles moved principally in a layer close to the pipe invert; however for higher and moderate flow velocities, particle saltation became the dominant mode of particle conveyance. Frictional pressure drops in the horizontal pipe were found to be markedly higher than in the vertical pipe, while the frictional pressure drops in the ascending pipe increased with inclination angle up to about 30°.
For the safe and economical design and operation of freight pipelines it is necessary to know slurry flow behaviour in inclined pipe sections, which often form significant part of pipelines transporting solids. Fine-grained settling slurry was investigated on an experimental pipe loop of inner diameter D = 100 mm with the horizontal and inclined pipe sections for pipe slopes ranging from –45° to +45°. The slurry consisted of water and glass beads with a narrow particle size distribution and mean diameter d50 = 180 μm. The effect of pipe inclination, mean transport volumetric concentration, and slurry velocity on flow behaviour, pressure drops, deposition limit velocity, and concentration distribution was studied. The study revealed a stratified flow pattern of the studied slurry in inclined pipe sections. Frictional pressure drops in the ascending pipe were higher than that in the descending pipe, the difference decreased with increasing velocity and inclination. For inclination less than about 25° the effect of pipe inclinations on deposition limit velocity and local concentration distribution was not significant. For descending pipe section with inclinations over –25° no bed deposit
was observed.
Gravity-driven open-channel flows carrying coarse sediment over an erodible granular deposit are studied. Results of laboratory experiments with artificial sediments in a rectangular tilting flume are described and analyzed. Besides integral quantities such as flow rate of mixture, transport concentration of sediment and hydraulic gradient, the experiments include measurements of the one-dimensional velocity distribution across the flow. A vertical profile of the longitudinal component of local velocity is measured across the vertical axis of symmetry of a flume cross section using three independent measuring methods. Due to strong flow stratification, the velocity profile covers regions of very different local concentrations of sediment from virtually zero concentration to the maximum concentration of bed packing. The layered character of the flow results in a velocity distribution which tends to be different in the transport layer above the bed and in the sediment-free region between the top of the transport layer and the water surface. Velocity profiles and integral flow quantities are analyzed with the aim of evaluating the layered structure of the flow and identifying interfaces in the flow with a developed transport layer above the upper plane bed.
Oil and gas are the main reserves in the fractures of rocks. Under different pressure conditions, fracture permeability of reservoir rock directly affects the flow of oil and gas, which is an important object of oil and gas exploration and development. The permeability of single fractured rock and double fractured rock under different pressure conditions was studied by using high-precision hydro-mechanics coupled triaxial experimental equipment. The experimental scheme is as follows: (i) permeability test under increasing confining pressure, (ii) permeability test under increasing liquid pressure, (iii) permeability test under cyclic loading and unloading deviatoric stress and (iv) permeability test under synchronously increasing confining pressure and deviatoric stress. Results show that the entire change of permeability is irreversibly reduced. This is due to the presence of residual factors in permeability after the dilation cycle and the recompaction cycle ends. On the basis of the dual medium model of fracture, the permeability model of fractured rock is proposed considering the interaction among fracture system, matrix system and expansion deformation of fracture under external stress. The simulation results of the model are in good agreement with the experimental results. These results can provide an important basis for the prediction of permeability of fractured rock and efficient oil and gas exploitation.
The paper presents selected experimental results for three-way exhaust gases catalytic reactor. Particular attention is paid to the difference in exhaust gases temperature measured at the catalyst inflow and outflow and the conversion of exhaust gases basic components. The energy balance proposed for gthe reactor takes into account the calculations of the effects of thermal reactions taking place in it. It is indicated that difference in temperatures of exhaust gases flowing through catalyst could be applied as a diagnostic signal.
Snow accumulation and melt are highly variable. Therefore, correct modeling of spatial variability of the snowmelt, timing and magnitude of catchment runoff still represents a challenge in mountain catchments for flood forecasting. The article presents the setup and results of detailed field measurements of snow related characteristics in a mountain microcatchment (area 59 000 m2 , mean altitude 1509 m a. s. l.) in the Western Tatra Mountains, Slovakia obtained in winter 2015. Snow water equivalent (SWE) measurements at 27 points documented a very large spatial variability through the entire winter. For instance, range of the SWE values exceeded 500 mm at the end of the accumulation period (March 2015). Simple snow lysimeters indicated that variability of snowmelt and discharge measured at the catchment outlet corresponded well with the rise of air temperature above 0°C. Temperature measurements at soil surface were used to identify the snow cover duration at particular points. Snow melt duration was related to spatial distribution of snow cover and spatial patterns of snow radiation. Obtained data together with standard climatic data (precipitation and air temperature) were used to calibrate and validate the spatially distributed hydrological model MIKE-SHE. The spatial redistribution of input precipitation seems to be important for modeling even on such a small scale. Acceptable simulation of snow water equivalents and snow duration does not guarantee correct simulation of peakflow at shorttime (hourly) scale required for example in flood forecasting. Temporal variability of the stream discharge during the snowmelt period was simulated correctly, but the simulated discharge was overestimated.
Acute lung injury in the preterm newborns can originate from prematurity of the lung and insufficient synthesis of pulmonary surfactant. This situation is known as respiratory distress syndrome (RDS). In the term neonates, the respiratory insufficiency is related to a secondary inactivation of the pulmonary surfactant, for instance, by action of endotoxins in bacterial pneumonia or by effects of aspirated meconium. The use of experimental models of the mentioned situations provides new information on the pathophy siology of these disorders and offers unique possibility to test novel therapeutic approaches in the conditions which are very similar to the clinical syndromes. Herewith we review the advantages and limitations of the use of experimental models of RDS and meconium aspiration syndrome (MAS) and their value for clinics., D. Mokra, A. Calkovska., and Obsahuje bibliografii