Experimental infection of immunocompetent and immunodeficient mice with Encephalitozoon cuniculi

Title:

Experimental infection of immunocompetent and immunodeficient mice with Encephalitozoon cuniculi

Creator:

Salát, Jiří, Braunfuchsová, Pavlína, and Kopecký, Jan

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7d08fffc-5b63-480b-915b-cd2629f2440c
uuid:7d08fffc-5b63-480b-915b-cd2629f2440c
doi:10.14411/fp.2001.041

Subject:

microsporidia, Encephalitozoon cuniculi, SCID mice, and NK cells

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

An experimental infection with the microsporidian Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923 was studied using a model of immunocompetent BALB/c mice and immunodeficient SCID mice. The course of infection after intraperitoneal inoculation of E. cuniculi spores was evaluated using the presence of spores in peritoneal macrophages as a criterion. First significant decrease in the proportion of infected cells was recorded on day 9 post infection (p.i.) in BALB/c mice. From day 14 p.i. no spores were observed in macrophages from BALB/c mice, while the number of infected macrophages from SCID mice increased until the death of the mice. The natural killer (NK) cell activity of mouse splenocytes was compared with the production of interferon gamma (IFN-γ) by these cells. While in BALB/c mice NK activity peaked on days 9 and 14 p.i., in SCID mice the marked increase of NK activity was recorded close before death of mice, on day 21 p.i. in correlation with the production of IFN-γ. Production of specific antibodies was demonstrated from day 9 p.i. in sera from BALB/c mice. It is concluded that intraperitoneal infection of SCID mice with spores of E. cuniculi results in the marked increase in the number of peritoneal exudate cells and in the percentage of infected cells close before death of mice. Neither high activity of NK cells nor increased production of IFN-γ are sufficient for the recovery of SCID mice from an E. cuniculi infection.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

249-254

Source:

Folia parasitologica | 2001 Volume:48 | Number:4

Harvested from:

CDK

Metadata only:

false