To study the light intensity suitable for Bletilla ochracea Schltr., morphology, photosynthetic parameters, and polysaccharide content of seedlings were evaluated under different light intensities. All shade treatments promoted plant growth and net photosynthetic rate while having no significant effect on transpiration rate. The maximum photochemical efficiency and potential photochemical efficiency reached the lowest values under full sunlight. The electron transport rate and photochemical quenching under shade were significantly higher than those under full light, while nonphotochemical quenching was the highest under full light. This indicated that the shade alleviated photoinhibition in summer and improved the utilization of light. B. ochracea could adapt to different light intensities, enhancing photosynthetic efficiency under low light by improving the electron transport and the degree of opened PSⅡ reaction centers, and adapting to high light by increasing heat dissipation. Plant growth, photosynthesis, and polysaccharide accumulation of B. ochracea greatly increased under 76.4% shade.
Diabetic nephropathy, included in diabetic kidney disease (DKD), is the primary disease leading to end-stage renal disease (ESRD) or dialysis treatment, accounting for more than 40 % of all patients with ESRD or receiving dialysis. Developing new therapeutics to prevent the transition to ESRD or dialysis treatment requires an understanding of the pathophysiology of DKD and an appropriate animal model for drug efficacy studies. In this study, we investigated the pathophysiology of diabetic kidney disease with type 2 diabetes in uninephrectomized db/db mice. In addition, the nephrectomized db/db mice from 10 weeks to 42 weeks were used to assess the efficacy of longterm administration of the angiotensin-II–receptor antagonist losartan. The blood and urinary biochemical parameters and the blood pressure which is a main pharmacological endpoint of the losartan therapy, were periodically measured. And at the end, histopathological analysis was performed. Uninephrectomized db/db mice clearly developed obesity and hyperglycemia from young age. Furthermore, they showed renal pathophysiological changes, such as increased urinary albumin-creatinine ratio (UACR) (the peak value 3104±986 in 40-week-old mice), glomerular hypertrophy and increased fibrotic areas in the tubulointerstitial tubules. The blood pressure in the losartan group was significantly low compared to the normotensive Vehicle group. However, as expected, Losartan suppressed the increase in UACR (829±500) indicating the medication was sufficient, but the histopathological abnormalities including tubular interstitial fibrosis did not improve. These results suggest that the uninephrectomized db/db mice are useful as an animal model of the severe DKD indicated by the comparison of the efficacy of losartan in this model with the efficacy of losartan in clinical practice.
In mammalian carnivore guilds (order Carnivora), spatiotemporal partitions play a major role in reducing competitive confrontations and facilitating successful sympatry. Using camera-trapping techniques, the present study aimed to elucidate patterns of spatial distribution and diel activities among medium- and large-sized carnivore species across central Bulgaria. We obtained 3,364 images of nine focal carnivores from 13,988 camera-trapping days between 2015 and 2020. Our findings indicated that the spatial distribution of the focal carnivore guilds varied with changes in altitudinal gradient, ruggedness, and forest-agricultural landscape changes. Specifically, the two largest species, the grey wolf (Canis lupus Linnaeus, 1758) and the brown bear (Ursus arctos Linnaeus, 1758), were found only in the Balkan Mountains, whereas the largest mesocarnivore, the golden jackal (Canis aureus Linnaeus, 1758), was mainly distributed agricultural lowlands. The European wildcat (Felis sylvestris Schreber, 1777) was found in forests inside protected areas, and other mesocarnivores were distributed at intermediate levels between wooded-mountains and agricultural lowlands. Brown bear, golden jackal, and domestic dog (Canis lupus familiaris Linnaeus, 1758) showed cathemeral, crepuscular, and diurnal activity, respectively, whereas the remaining six carnivores showed nocturnal activity in synchrony with their main prey. Our findings indicated that anthropogenic landscape modifications and potential interspecific competition resulted in patterns of spatial distribution and temporal activity in this carnivore guild.
Objectives. This study aims to better understand peak experience and their varieties (PEV), as a key concept in understanding positive human experience, in the context of a specific adventurous recreational pursuit. Sample and settings. Interviews with the 14 crew members of the historical sailing vessel Oesterscheldewere held at the end of a 38-day crossing of the Indian Ocean. A rigorous qualitative hermeneutical – narrative approach in analysing interviews was utilised. Results. The results were based on a qualitatively different cross-section of PEV that could be seen as a mixture of already thematised varieties of peak experience. The PEV were presented on three levels: the level of action, the level of feeling and the level of reflection; as such, they consisted of the interplay between specific challenges (situations) and responses (reactions). Alongside original methodology and analysis, the authors suggested the importance of studying the participant’s response to PEV. The response to PEV is seen as a crucial component in this study and to PEV itself. Finally, it was suggested that PEV could be projected into the future as a private and useful model of participation in life. Limitations. The study has a number of limitations commonly connected to the qualitative approach: a small number of participants, language bias (all interviews were held in English), the bias of question structure (“what did you feel?”), the participation of a researcher on the voyage, and the professionalism of the main crew that could bias the interviews. and Cílem této studie bylo lépe porozumět vrchol-ným zážitkům a jejich variacím (PEV), jakožto klíčovému konceptu pro pochopení pozitivních lidských prožitků, v kontextu specifického dob-rodružného rekreačního vyžití. Rozhovory se 14 členy posádky historické plachetnice Oester-schelde se uskutečnily na konci 38denní plav-by přes Indický oceán a k jejich analýze autoři využili kvalitativní hermeneuticko-narativní přístup. Výsledky vycházely z kvalitativně odlišného průřezu PEV, který lze považovat za směs již te-matizovaných variací vrcholného zážitku. PEV byly prezentovány ve třech rovinách: v rovině jednání, v rovině pocitů a v rovině reflexe; jako takové se skládaly ze souhry konkrétních výzev (situací) a reakcí (odpovědí). Kromě originální metodiky a analýzy autoři zdůrazňují význam studování odpovědí účastníků na PEV. Reakci na PEV považují za klíčovou složku této studie i PEV samotné.Studie má řadu omezení běžně spojených s kvalitativním přístupem: malý počet účastníků, jazykové zkreslení (všechny rozhovory probíha-ly v angličtině), zkreslení dané strukturou otá-zek rozhovoru a také faktem, že tazatel se také účastnil plavby.
Deposition of solid particles in the stormwater sewers reduces the discharging capacity, causing inundation. A sediment invert trap (SIT) is an option that can be installed at the bottom of the stormwater sewer drain to intercept the flowing solid particles. In the present study performance of rectangular SIT were analyzed experimentally and computationally. Variation of particle trapping efficiency of rectangular SIT fitted at the bottom of the open channel flume has been studied under the interpretation of invert trap depth, flow depth, particle size, particle shape, and slot width. To predict the flow field and trap efficiency of a rectangular invert trap, 2D-VOF-DPM-CFD modelling has been carried out using ANSYS Fluent 2020 R1 software. For velocity field determination, the volume of fluid (VOF) model was used along with realizable k-є turbulence model. To predict particle trap efficiency, stochastic discrete phase model (DPM) was utilized. From experimental study and CFD modeling, it has been found that the particle trap efficiency of rectangular invert trap varied with change in the depth of invert trap, sediment size, shape factor, depth of flow and slot width. Consideration of particle shape in terms of shape factor in the modeling of solid-phase through DPM validated the CFD predicted results with those obtained experimentally with mean absolute percent error (MAPE) of 2.68%, 3.99% and 6.6% for sewer solid size ranges SS1, SS2, and SS3 respectively at all flow depths for both slot widths considered in this study.
Detailed data on the long-term performance of bioretention cells (BC) for stormwater management are sparse. This research aimed at setting up and testing an infrastructure that will provide the data on hydrologic and chemical performance of BC. Two identical experimental BC’s were built. The monitoring methodology monitoring was developed and tested during a first growing season with the first BC supplied with natural rainfall, while the second BC was used for ponding experiments. Key layer of the BCs, a biofilter, was composed of sand, compost and topsoil. Both BCs are equipped with sensors monitoring the components of water balance and the water potential of the biofilter. High levels of total suspended solids were detected in the outflow. The runoff coefficient for the entire period of the growing season was 0.72 in the first BC and 0.86 in the second BC, while the peak outflow reduction for individual rainfall episodes ranged between 75% to 95% for the first BC and 19% to 30% for the second BC. Saturated hydraulic conductivity of the biofilter in the first BC decreased by two orders of magnitudes after the first year of operation. Retention curves of the biofilter changed due to material consolidation.
Balance control is a critical task of daily life, the ability to maintain upright posture becomes of particular concern during aging when the sensory and motor system becomes deteriorated. Falls contribute to the most deaths caused by injury within the aged population, and the mortality rate following a fall is drastically elevated. Longitudinal and reliable assessment of balance control abilities is a critical point in the prediction of increased risk of falling in an elderly population. The primary aim of the study was to evaluate the efficiency of the Homebalance test in the identification of persons being at higher risk of falling. 135 subjects (82 women and 53 men) with geriatric syndrome have been recruited and the Homebalance and the Tinetti Balance test were performed. Results of both tests strongly correlated proving the good performance of the Homebalance test. Standing balance declines with increasing body mass index in both genders. Analysis of fluctuations of the center of pressure (COP) revealed higher frequency and magnitude in mediolateral direction COP movements when compared women to men. A strong negative correlation has been found between Tinetti static balance score and the total length of the COP trajectory during the examination on Homebalance (r = -0.6, p<0.001). Although both methods revealed good performance in detecting balance impairment, Homebalance test possesses higher precision due to the continuous nature of COP-derived parameters. In conclusion, our data proved that the Homebalance test is capable to identify persons with impaired balance control and thus are at higher risk of falling.
Vascular calcification (VC) is an independent risk factor for cardiovascular events and all-cause mortality with the absence of current treatment. This study aimed to investigate whether eIF2α phosphorylation inhibition could ameliorate VC. VC in rats was induced by administration of vitamin D3 (3×105 IU/kg, intramuscularly) plus nicotine (25 mg/kg, intragastrically). ISRIB (0.25 mg/kg·week), an inhibitor of eIF2α phosphorylation, ameliorated the elevation of calcium deposition and ALP activity in calcified rat aortas, accompanied by amelioration of increased SBP, PP, and PWV. The decreased protein levels of calponin and SM22α, and the increased levels of RUNX2 and BMP2 in calcified aorta were all rescued by ISRIB, while the increased levels of the GRP78, GRP94, and C/EBP homologous proteins in rats with VC were also attenuated. Moreover, ISRIB could prevent the elevation of eIF2α phosphorylation and ATF4, and partially inhibit PERK phosphorylation in the calcified aorta. These results suggested that an eIF2α phosphorylation inhibitor could ameliorate VC pathogenesis by blocking eIF2α/ATF4 signaling, which may provide a new target for VC prevention and treatment.
Two light treatments [ambient sunlight (L1) during the entire growth period and 40% shade (L2) from 40 d after sowing until 24 d after flowering] and two phosphate fertilizer treatments [no phosphate fertilizer application (P0) and a conventional phosphate fertilizer application (P1)] were used to determine how phosphate fertilizer regulates soybean [Glycine max (L.) Merr.] photosynthesis under shading. We showed that phosphorus significantly increased chlorophyll content and grain yield under shading. The light-saturated net photosynthetic rate, apparent quantum yield, maximum electron transport rate, and maximum Rubisco carboxylation rate in P1 under L2 significantly increased. Moreover, phosphate fertilizer significantly improved the electron transfer and PSII reaction center performance under shading. Therefore, phosphate fertilizer increases low light-utilization efficiency by improving PSII performance, promoting ribulose-1,5-bisphosphate regeneration, ensuring a source of carboxylate substrates, and coordinating the balance between photochemical reaction and Calvin cycle under shading.