Úvod: Odběr ledvin z dárce s nezvratnou zástavou oběhu (DCD – donor after circulatory death) je důležitou součástí transplantačních programů v celosvětovém měřítku. V České republice byla první transplantace ledvin z DCD úspěšně provedena v roce 2002. Autoři diskutují vlastní a literární zkušenosti s tímto programem. Snahou je zvýšit potenciál odběru ledvin z DCD v České republice. Metoda: Od roku 2002 do roku 2015 bylo v plzeňském Transplantačním centru odebráno celkem 44 ledvin z DCD. Technikou byl „in situ“ odběr pomocí „double baloon triple lumen“ katetru při zachování 5–10 min. „no-touch“ intervalu. Metoda hypotermické pulzatilní perfuze byla použita k testování viability odebraných ledvin. Transplantováno bylo celkem 28 ledvin příjemcům o průměrném věku 51,1 roku (26–73 let). Dle maastrichtských kritérií bylo 16 (57,1 %) ledvin ve skupině II, 8 (28,6 %) ve skupině III a 4 (14,3 %) ve skupině IV. Výsledky: 30denní pooperační mortalita byla 0 a morbidita 10,7 tj. 14,3 % (N=4). Primární afunkce ledviny byla přítomna u 2 (7,1 %), opožděný nástup funkce pak u 5 (17,9 %) nemocných. Jeden rok, 5 a 10 let po transplantaci žije 100 %; resp. 86,4 % a 76,7% nemocných a ve stejných intervalech je funkčních 92,9; 69,6 a 61,9 % transplantovaných ledvin. Dlouhodobé výsledky jsou plně srovnatelné s transplantacemi ledvin od dárců se smrtí mozku. Závěr: DCD jsou významným zdrojem ledvin pro transplantace. Transplantace ledvin z DCD je metodou logisticky, ekonomicky a personálně náročnou s velmi dobrými dlouhodobými výsledky., Introduction: Kidney procurement from donors after circulatory death (DCD) is an important part of worldwide transplantation programmes. The first kidney transplantation from DCD was successfully performed in the Czech Republic in 2002. Method: Forty four kidneys from DCD were procured in the Transplant Centre of Pilsen between 2002 and 2015. We used the technique of “in situ“ procurement with the double balloon triple lumen catheter and 5−10 minutes of the no-touch interval. The method of pulsatile hypothermic perfusion was used to test the viability of the kidneys. Twenty eight recipients with mean age 51.1 (26−73) years were transplanted. Sixteen (57.1%) kidneys were from the 2nd, 8 (28.6%) from the 3rd and 4 (14.3%) from the 4th category according to the Maastricht criteria. Results: 30-day mortality and morbidity rates were 0 and 10.7% i. e.14.3% respectively (N=4). Primary non-function was presented in 2 (7.1%), and delayed graft function in 5 (17.9%) cases. One, five and ten years of recipient and graft survival rates were 100%, 86.4% and 76.7%; and 92.9%, 69.6% and 61.9%, respectively. The long-term results are fully comparable with kidneys transplanted from donors after brain death. Conclusion: DCD are an important source for kidney transplantation. Kidney transplantation from DCD is a logistically, economically and personally demanding method with very good long-term results., and V. Třeška, T. Reischig, D. Hasman, B. Čertík, J. Moláček, R. Šulc, M. Čechura, L. Kielberger, K. Houdek, V. Opatrný
The Institute of the Rock Structure and Mechanics AS CR operates the GEONAS network that now consists of 17 perm anent GPS observatories. The outliers and in consistencies occur within the time series observed in the winter season 200 5/2006 for the position of the GNSS antennas of the observatories SNEC and BISK located high in the m ountains, at th e Sněžka Mt. (1602 m, the Giant Mts.) and the Biskupská kupa Mt. (890 m, the Jeseníky Mts.) respectively. Therefore web cameras and meteorological sensors were in stalled at GEONAS observatories located in the mountain regions. The snow coverage and other meteorological influences affecti ng the antennas monitoring GPS signals at these observatories were estimated. The individual photos were analyzed and compared to variations in the time series to obtain the time series for winter seasons reducing the snow coverage effects., Milada Grácová, František Mantlík, Vladimír Schenk and Zdeňka Schenková., and Obsahuje bibliografické odkazy
The essential point for seismological observation in the northern part of Moravia, Czech Republic, is a permanent seismic station Ostrava - Krásné Pole (OKC) that is a part of the Czech regional seismological network (hereafter CRSN). Institute o f Geonics AS CR, v.v.i., has also operated temporary seismic stations in this region since 1997. Current seismological stations are located in an abandoned mine working located in Zlaté Hory and in cellars in Klokočov and castle Raduň near Opava. The natural seismicity is after the swarm of microearthquakes in the vicinity of Opava in 1993 very low now. In region under discussion, seismic manifestations generated by mining induced seismicity in Upper Silesian Coal Basin (both Karviná an d Polish parts) and in Legnica-Głogów Copper District (LGOM) are also detected. Significant part of observations in this region is made by the Institute of Physics of the Earth (hereafter IPE), Masaryk University Brno, and about 1000 microearthquakes have been detected during the last ten years with maximum local magnitude up to 1.9., Zdeněk Kaláb, Jaromír Knejzlík and Karel Holub., and Obsahuje bibliografické odkazy
A theoretical model is presented, which attempts to account for the evaluation of complex stimuli in terms of their constituent elements that are relevant to the intent of the assessment. The subjective evaluation of a compound stimulus is postulated to be a function of the number, weight and integrity of critical components, or sub-qualities, and their interactions. The model has application to the evaluation of any stimulus complex including works of “art”. For illustrative purpose, it will here be applied to the analysis of pictorial works of art., J. P. Huston., and Obsahuje bibliografii
This work discusses the clinical performance of deconjugated metanephrine (MN), normetanephrine (NMN) and 3-methoxytyramine (3MT) determined in the basal first morning urine using a chromatographic method with electrochemical detection for the clinical diagnosis of pheochromocytoma (PHEO) and paraganglioma (PGL). Urine samples were collected from 44 patients (36 with PHEO, 8 with PGL) aged 54+/-17 (20-78) years (22 females, 22 males). A sampling of biological materials was performed preoperatively and about one week, six months and one year after adrenal gland surgery. The control group consisted of 34 PHEO/PGL patients more than 4 months after adrenal gland surgery. All subjects in the control group were without a diagnosis of PHEO or PGL. Clinical sensitivity was 55 % for MN, 64 % for NMN, 80 % for combination of both MN and NMN, and only 23 % for 3TM. Clinical specificity calculated from the control group was 93 % for MN, 95 % for NMN, 95 % for the combination MN and NMN, and 97 % for 3TM. Cut-off values for deconjugated metanephrines in the basal urine were 310 (MN), 690 (NMN) and 250 μg/l (3MT). Chromatographic determination of deconjugated urinary metanephrines, which is simple without the necessity of special laboratory material, can serve for the screening of PHEO or PGL patients. Urine NMN and 3MT exerts an association to malignity, and all markers are associated with tumor mass. However, the principal laboratory diagnosis of PHEO or PGL must be based on plasma-free metanephrines and plasma chromogranin A with better performance in the laboratory diagnosis of PHEO or PGL., R. Bílek, T. Zelinka, P. Vlček, J. Dušková, D. Michalský, K. novák, J. Bešťák, J. Widimský Jr., and Obsahuje bibliografii