Antiendothelial cell antibodies (AECA) have been detected by flow cytometry analysis in 23 out of 80 patients with connective tissue diseases. Ten out of 19 serum samples from patients with systemic lupus erythematosus (SLE) were positive. These antibodies were not detectable in healthy donors. We examined the capacity of serum samples to induce endothelial cell activation by modulating cell adhesion molecule expression on human umbilical vein endothelial cells. We found that sera from both AECA-positive and AECA-negative patient groups induced a significantly higher expression of E-selectin compared to healthy controls (P<0.05). There were no differences in the ICAM-1 on VCAM-1 expression. Our data suggest that increased E-selectin expression in activated endothelium in patients with various connective tissue disorders is not related to the production of AECA., M. Horváthová, E. Jahnová, Š. Nyulassy., and Obsahuje bibliografii
The electrophoretic migration rates of several proteins of photosystem 2 particles ffom spinách were much higher iii gels containing 1 mM Ca^^ than in gels containing 1 mM ethyleneglycol-í)w(P-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA). Incubation of gels with terbium (Tb^^) and the corresponding Tb3‘'^-fluorescence were ušed to identity the Ca^^-binding proteins on the basis of selective occupation of Ca2+-binding sites with Tb^+. The 47, 43 and particularly 33 kDa polypeptides were most probably involved in Ca2+-binding.
BACKGROUND: The goal of this prospective study was to determine the frequency of micrometastases in patients with squamous cell carcinoma (SCC) of the oral cavity, pharynx and larynx in whom elective neck dissection was indicated (cN0). PATIENTS AND METHODS: A total of 12 patients (10 males and 2 females) were enrolled in the study. The age ranged 42-73 years (median 62 years). Elective neck dissection was performed in all patients (8 ipsilateral, 4 bilateral) and a total of 256 lymph nodes were removed and sent for microscopic examination. RESULTS: The presence of tumor cells in cervical lymph nodes was found in 5/12 (42%) patients. Micrometastases of SCC were found in two patients and isolated tumor cells (ITC) in two other patients. In the remaining one patient with oropharyngeal SCC, a micrometastasis of papillary thyroid carcinoma (PTC) was detected. Positive lymph nodes were localized in level II in three patients with SCC of larynx, hypopharynx and tongue base, respectively, in level I in one patient with SCC of oral tongue and in level III in one patient with PTC. CONCLUSION: Our results indicate that SCC of head and neck has a high potential for creating micrometastases which frequency is higher compared to clinically detected macrometastases. Therefore, elective neck dissection or radiotherapy of the neck should be considered in patients with high risk of occult metastases or micrometastases. and P. Čelakovský, D. Kalfeřt, K. Smatanová, V. Chrobok, J. Laco
Based on the analysis performed in the Kura geomorphological sub-region of the Caspian coastal zone, it was determined that the sea level rise of 2.43 m in 1976-1996 caused an average annual rate of erosion processes about 75 times higher than accumulation processes. In 1996-2019, the stabilization and the following drop of the sea level of 1.39 m led to an average annual rate of accumulation processes up to 14 times higher than the average annual rate of erosion processes. This pattern is observed along the entire coastline of those periods. Erosion processes in the initial stage (1976-1996) increased the indentation and length of the coastline by almost 2 times, and in the next stage (1996-2019) the intensification of accumulation processes resulted in the smoothing and reduction of the coastline by 35 %. Statistical analysis of coastlines was carried out by processing Landsat satellite images for 1976-2019; each coastline was divided into 14 segments according to geomorphological zones and their morphostructures in the study area. The development forecast for the next 10 and 20 years is provided., Jeyhun Yashar oglu Gasimov., and Obsahuje bibliografii
We have surveyed the Earth's surface using gravity anomalies and second-order radial derivatives of the disturbing gravitational potential computed from the gravitational model EGM2008 complete to degree and order 2159 (for selected degrees up to 2190). It corresponds to 5 arcmin resolution on the ground. Over most well known impact crater sites on the Earth we found the second-order derivatives (not available from ordinary gravity surveys) offered finer discrimination of circular features than the gravity anomalies themselves. We also discovered that some of the sites show evidence of double or multiple craters which will need further ground verification. Some of these signatures (in hilly or mountainous terrain) may also need to be corrected for the gravitational effect of topography to sharpen their hidden features., Jaroslav Klokočník, Jan Kostelecký, Pavel Novák and Carl A. Wagner., and Obsahuje bibliografii
a1_The wide chemical diversity of estrogenic compounds precludes an accurate prediction of estrogenic activity on the basis of chemical structure or radioimmunological assay and thus requires that the potency of these compounds is defined by bioassay. The mammary duct growth response in intact prepubertal and adult gonadectomized female and male mice of the C3H/Di strain was used to assess the estrogenicity of synthetic compounds or their derivatives. The vehicle for tested compounds should be free of estrogenic and other hormonal effects. Olive oil or sunflower oil exerted estrogenic activities and were thus unsuitable as vehicles for the tested compounds. The absence of estrogenic activity, high solubility of different steroid hormones, and the low incidence of the inflammatory reactions at the injection site were achieved by using a vehicle containing benzyl alcohol, benzyl benzoate, butylhydroxyanisole, butylhydroxytoluene, ethyl oleate and ethanol. The bioassay was primarily designed to examine the effect of tested compounds on mammogenesis. The duration of hormone treatment was chosen to be long enough for induction of duct growth but too short to induce lobuloalveolar differentiation. Females were treated for 10 days, males for 15 days. The proportional volume occupied by mammary epithelial structures was estimated by the modified Chalkley's technique. The mean coefficient of variation of quantitative evaluation of 10 different mammary glands obtained by two operators varied between 3.2 and 17.4 %. The mean coefficient of variation of quintuplicate determinations of each mammary gland by one operator was 10.1 %, and 11.1 % by the other. The correlation coefficient between results of two operators was 0.994. Estrogens are primarily defined by their ability to increase the mitotic activity of female secondary sex organs., a2_However, our results have shown that progesterone alone, if administered in a high dose, stimulates mammary growth in both intact prepubertal and OV-X female mice similarly as the synthetic progestatial steroid norethindrone with inherent estrogenic properties. In contrast, progesterone alone had no effect, in young intact or adult castrated males, but norethindrone did stimulate mammary growth. These results demonstrated that the mammary gland of males is a suitable model for estrogen screening., J. Škarda., and Obsahuje bibliografii
This paper presents results from the exam ination of flood embankments by means of three geophysical methods: GPR, mutual impedance of loop antennas measurements and D.C. resistivity method. In order to increase measuring accuracy, the mutual impedance measuring system works at a high frequency. Parameters of mutual impedance measuring system were presented. A method of mutual im pedance measurement results was described. Flood embankments examination results showed that the simultaneous use of few geophysical methods increases accuracy of inhomogeneities detection in near-surface structure of the ground., Remigiusz Mydlikowski, Grzegorz Beziuk and Adam Szynkiewicz., and Obsahuje bibliografické odkazy
The presence of Neospora caninum Dubey, Carpenter, Speer, Topper et Uggla, 1988 in small mammals (i.e. murid rodents, Erinaceomorpha, Eulipotyphla and Scadentia) was explored for first time in South-East Asia. A total of 192 individuals from six localities across Thailand were analysed. A general prevalence of N. caninum of 22% was observed, with some variation among localities (5-36%). Four main types of habitat were included and rodents trapped in dry-land habitat (17 positive among 41 individuals) were more likely to be infected with N. caninum than those from other habitats (forest, rain-fed land and settlement). Rodent species identity and individual rodent weight had no influence on individual infection. Our results provided the first data on the presence of N. caninum in rodents in South-East Asia and first report of N. caninum in the order Scadentia., Ornampai Japa, Serge Morand, Anamika Karnchanabanthoeng, Kittipong Chaisiri, Alexis Ribas., and Obsahuje bibliografii
Fluorescence in situ hybridization (FISH) is a technique used to determine the chromosomal position of DNA and RNA probes. The present study contributes to knowledge on jumping plant-lice genomes by using FISH with 18S rDNA and telomeric (TTAGG)n probes on meiotic chromosomes of Psylla alni (2n = 24 + X), Cacopsylla mali (2n = 22 + neo-XY and 20 + neo-X1X2Y), C. sorbi (2n = 20 + neo-XY), Baeopelma foersteri (2n = 14 + X), and Rhinocola aceris (2n = 10 + X). This is the first study that has used FISH on the hemipteran superfamily Psylloidea. We found that the chromosomes of all studied species contain the insect-type telomere motif, (TTAGG)n. In C. mali and C. sorbi, the neo-sex chromosomes originating from autosome-sex chromosome fusions showed no interstitially located clusters of TTAGG repeats, suggesting their loss or inactivation. Similarly, no interstitial (TTAGG)n clusters were detected in an extremely large autosome pair of B. foersteri that most likely originated from a fusion of at least five ancestral chromosome pairs. Clusters of 18S rDNA were detected on the fused and second largest autosome pairs of B. foersteri and on one of the large autosome pairs of the remaining species. In C. mali and B. foersteri, the rDNA clusters were shown to coincide with the NORs as detected by the AgNOR method. Finally, we speculate, based on the obtained FISH markers, on the mechanisms of karyotype evolution of psylloid species differing in chromosome numbers and sex chromosome systems., Anna Maryańska-Nadachowska, Valentina G. Kuznetsova, Natalia V. Golub, Boris A. Anokhin., and Obsahuje bibliografii