Detection of Antiendothelial Cell Antibodies in Patients with Connective Tissue Diseases by Flow Cytometry and Their Relation to Endothelial Cell Activation

Title:

Detection of Antiendothelial Cell Antibodies in Patients with Connective Tissue Diseases by Flow Cytometry and Their Relation to Endothelial Cell Activation

Creator:

Marcela Horváthová, Eva Jahnová, and Štefan Nyulassy

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:63b8bd49-5551-47b8-bd57-c6e97d665e76
uuid:63b8bd49-5551-47b8-bd57-c6e97d665e76
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Antiendothelial cell antibodies, Adhesion molecules, Expression, Flow cytometry, 14, and 612

Type:

article, studie, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Antiendothelial cell antibodies (AECA) have been detected by flow cytometry analysis in 23 out of 80 patients with connective tissue diseases. Ten out of 19 serum samples from patients with systemic lupus erythematosus (SLE) were positive. These antibodies were not detectable in healthy donors. We examined the capacity of serum samples to induce endothelial cell activation by modulating cell adhesion molecule expression on human umbilical vein endothelial cells. We found that sera from both AECA-positive and AECA-negative patient groups induced a significantly higher expression of E-selectin compared to healthy controls (P<0.05). There were no differences in the ICAM-1 on VCAM-1 expression. Our data suggest that increased E-selectin expression in activated endothelium in patients with various connective tissue disorders is not related to the production of AECA., M. Horváthová, E. Jahnová, Š. Nyulassy., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2002 Volume:51 | Number:6

Harvested from:

CDK

Metadata only:

false