The ubiquitin-proteasome pathway fulfills major biological functions, but its physiologic tissue distribution and the interrelationship between pathway component activities and ubiquitin pools are unknown. Therefore, we analyzed free and conjugated ubiquitin, ubiquitin-protein ligation rates (UbPL) and chymotryptic- and tryptic-like proteasome peptidase activities in porcine skeletal muscle, heart, lung, liver, spleen and kidney (n=5 each). There were considerable differences between tissues (p<0.05 for all parameters). Lung and spleen showed high levels of free and conjugated ubiquitin and high UbPL. Proteasome activities were highest in kidney and heart. There were linear relationships between tryptic-like and chymotryptic-like proteasome peptidase activities (r2 = 0.624, p<0.001) and between free and conjugated ubiquitin tissue levels (r2 = 0.623, p<0.001). Tissue levels of free and conjugated ubiquitin correlated linear with UbPL (p<0.005), but they were not correlated with proteasome peptidase activities. The results suggest that tissue ubiquitin pools are tightly regulated and indicate a constant proportion of conjugated ubiquitin. They further support the hypothesis that ubiquitin-protein ligase systems, and probably deubiquitylating enzymes, are key regulators of ubiquitin homeostasis. The detected differences are suggestive of tissue-specific roles of ubiquitin-proteasome pathway components. Besides the known importance of the ubiquitin proteasome pathway in heart, kidney and the immune system, the results suggest the lung as another organ in which ubiquitin proteasome pathway components may also significantly contribute to disease processes., M. B. Patel, M. Majetschak., and Obsahuje bibliografii a bibliografické odkazy
A complete survey is presented of photosynthetic patfaway in the 27 species of the genus Cyperus occurring in Europe. Among them, tíiere are 20 species vsdth C4 photosynthesis and 7 species with C3 photosynthesis. In 14 species the pathway is repoited for the first time. Wifli different pattems, both C3 and C4 types occur from the Mediterranean region (35 °N) to the cool temperate region (52 “N). Atlantic current may háve a striking effect on the distňbution of wetland C4 sedges. Although the existence of rhizomes in C4 Cyperus perennials favours their survival at short summers of centra! and west Europe, low temperature at latě summer in the regions prevents them to complete seed development. Leaf anatomy indicates that diy summer in Mediterranean regions limits some of them due to dieir lavish water requirement. In the eastem and north Europe a long period of low temperature in winter may be the most important reason for the absence of C4 Cyperus species.
The 77 K fluorescence emission was measured on Chlorella cells at regular intervals during adaptatíon of high CO2 (1.8 % CO2 v/v) grown cells to air level of CO2. The ratíos of the peaks at 686 and 697 nm (photosystem 2, PS 2, fluorescence) to the peak at 720 nm (PS 1 fluorescence) decreased and approached a constant level in about 1.5 h. The extemal concentration of dissolved inorganic carbon (DlC) required for the half-maximum rates of C02-dependent O2 evolutíon, 5, also decreased and reached a constant level aíter the same period of adaptatíon. Hence the íncreased demand for ATP/NADPH during the CO2 assimilatíon by the ''C02-concentrating mechanism" induces the redistribution of excitation energy from PS 2 to PS 1 and increases formation of ATP by the cyclic photophosphorylatíon.
Combustion of lignite with limestone, co-combustion of lignite with limestone and wood, and combustion of wood were performed in a circulating fluidised bed in 7 different combustion regimes. The in organic matter composition and properties of all input and output materials were characterized. For each com bustion regime the material streams and the boiler outputs were calculated. The total inorganic mass and the mass of selected trace elements in fuels (Cl, Zn, As, Se, Hg, and Pb) were based on the output of a 1 GW circulating fluidised-bed boiler; additionally, the concen trations of volatile elements (As, Se, Hg) in fly ash (FA) were evaluated in relation to the mass of FA. Element (Cl, Zn, As, Se, Hg, and Pb) enrichment in FA compared with BA was related to the ratio R between the co mbusted mass of organic wood wastes and total combusted fuel. Additionally, the unburned carbon in bottom ash (BA) and fly ash (FA) was determined, and sel ected elements were studied in unburned materials separated from BA. The results show that combustion of wood and/or co-combustion of lignite with wood waste brings about significant environmental benefits., Zdeněk Klika, Lucie Bartoňová and Jana Serenčíšová., and Obsahuje bibliografické odkazy
Nearly all epileptic seizures in patients are characterized by deranged consciousness. We started to study changes in motivated behavior (drinking in thirsty rats) as a possible analogue of compromised consciousness during and after epileptic seizures. Epileptic afterdischarges (ADs) were elicited by stimulation of the dorsal hippocampus and/or thalamus. Rats with implanted electrodes (deprived of water for 24 hours) were trained to lick water from a narrow tube. After pretraining ADs were elicited eight times in each animal and access to water was allowed during different phases of the AD. Stimulation did not affect licking if no AD was induced. If stimulation was successful, licking was stopped in nearly 70 % of stimulations and modified (biting the tube) in 30 %. Hippocampal ADs (characterized by serrated waves in the EEG and by an arrest of behavior with subsequent automatisms) completely blocked licking, signs of recovery appeared during the interval between the AD and recurrent AD and it progressed during recurrent ADs. Thalamic ADs abolished licking in 82% of cases and immediately after ADs normal licking reappeared in 49 % of these observations. Our results suggest that changes in motivated behavior might serve as an analogue of compromised human consciousness., P. Mareš, L. Chocholová., and Obsahuje bibliografii
Extremely low-frequency magnetic field (ELF-MF) has been suggested to influence the cognitive capability but this should be dynamically evaluated in a longitudinal study. Previous training can affect performance, but the influence under magnetic field is unclear. This study aims to evaluate the effects of previous training and ELF-MF exposure on learning and memory using the Morris water maze (MWM). Sprague-Dawley rats were subjected to MWM training, ELF-MF exposure (50 Hz, 100 μT), or ELF-MF exposure combined with MWM training for 90 days. Normal rats were used as controls. The MWM was used to test. The data show that the rats exposed to training and ELF-MF with training performed better on spatial acquisition when re-tested. However, during the probe trial the rats showed no change between the training phase and the test phase. Compared with the control group, the ELF-MF group showed no significant differences. These results confirm that previous training can improve the learning and memory capabilities regarding spatial acquisition in the MWM and this effect can last for at least 90 days. However, this improvement in learning and memory capabilities was not observed during the probe trial. Furthermore, ELF-MF exposure did not interfere with the improvement in learning and memory capabilities., Y. Li, C. Zhang, T. Song., and Obsahuje bibliografii
Předkládaná studie se věnuje specifikům dětské výpovědi. Mapuje možné zdroje její nespolehlivosti (nízká schopnost zapamatovat si a vybavit si události, vysoká sugestibilita, snadnost vzniku falešných vzpomínek, neporozumění pro procesy a interakce v okolí, možnost záměrného lhaní). Jsou uvedeny některé postupy, které mohou pomoci zkvalitnit, nebo naopak znehodnotit informace, podané dítětem. Schopnost dítěte porozumět své vlastní motivaci, prožívání a chování, stejně tak jako chování a prožívání jiných lidí je často zpochybňována. Ukazuje se však, že dětský úhel pohledu na události, které zažívá, a na sociální interakce, kterým je vystaveno, je obohacující. Přináší smysluplné informace, je jedinečný a přispívá jak k pochopení prožívání a chování dítěte a jeho aktuálních potíží, tak ke hlubšímu porozumění rodinným vztahům a jiným skutečnostem., The present study maps the possible sources of unreliability of child,s testimony (lower ability to remember and recall events, high suggestibility, easier formation of false memories, misunderstanding of the processes and interactions in surroundings, probability of deliberate lying) as well as its potential benefits in research and counselling practices. There are some procedures that can help improve or conversely depreciate information given by child. Child,s ability to understand their own motivation, experience and behaviour as well as behaviour and feelings of others are often questioned. But despite all mentioned risks, child,s testimony is very useful not only in judicial proceedings in which the child may be the only witness to the events but also in psychological research and counselling. It turns out that a child is more sensitive to some aspects of family life (conflicts) than an adult and gives even more accurate information than its parents. Child,s view of family life is important for understanding its behaviour and experience., Xenie Uholyeva., and Obsahuje seznam literatury
The aim of the present paper is to examine certain philosophical issues which have set the tone of the philosophical reflection in eighteenth century France in relation to a specific case study: that of the "wild child" known as Victor of Aveyron. Found in 1800 in central France, Victor was later transferred to the Parisian Institute of the DeafMutes, where he became the object of educational activities of JeanMarc Itard, a medical expert known for his works on the problem of hearing loss. Through a brief critical examination of the most notorious philosophical texts dealing both with the question of wild children and deafness (namely by Rousseau, Diderot and Condillac), we attempt to show that the specificity of Itard’s educational method consists in an application of the sensualist approach towards the human individual (as it is exemplified especially in the work of Condillac) on a concrete human subject, considered as a tangible proof of the inexistence of innate ideas. On this basis, we sketch several broader questions concerning the status of anomaly in the eighteenth century philosophical thought (namely, wild children and deafness), as well as some hypotheses on education and its fantasmatic aspects in general., Josef Fulka., and Obsahuje bibliografické odkazy
In order to use rationally switchgrass (Panicum virgatum L.) introduced in a large scale in semiarid regions on the Loess Plateau of China, we investigated and compared soil water storage dynamics, diurnal and seasonal changes in leaf photosynthetic characteristics, and biomass production of switchgrass grown under three different row spacing (20, 40, and 60 cm). Results indicated that photosynthetic parameters showed a pronounced seasonality. Diurnal course of net photosynthetic rate (P N) was bimodal, showing obvious midday depression, which was mainly due to stomatal limitation in May and June, by nonstomatal limitation in August, and both stomatal and nonstomatal factors in September. Generally, P N, stomatal conductance, instantaneous water-use efficiency, light-saturated net photosynthetic rate, saturation irradiance, and compensation irradiance increased with increasing row spacing. Plant height, leaf width, and a relative growth rate of biomass accumulation were significantly higher at the row spacing of 60 cm, while 20 cm spacing showed significantly higher aboveground biomass production and the biomass water-use efficiency. All these confirmed that soil water is the key limiting factor influencing switchgrass photosynthesis, and suggested that the wide row plantation (i.e., 60 cm) was more beneficial to switchgrass growth, while narrow spacing was in favor of improving switchgrass productivity and water-use efficiency., Z. J. Gao, B. C. Xu, J. Wang, L. J. Huo, S. Li., and Obsahuje seznam literatury