We investigated the gastric response to an ulcerogenic irritant and the change in gastric functions in an experimental rat model of obstructive jaundice, with or without biliary drainage. After biliary obstruction for 14 days, rats with ligated bile duct (BDL) were randomly divided into three groups: BDL group without biliary drainage, BDL followed by choledochoduodenostomy (CD) or a choledochovesical fistula (CVF). The gastric functions were evaluated 2 weeks after the surgery. Gastric damage, induced by orogastric administration of ethanol, was evaluated 30 min later using a lesion index and microscopic scoring was then performed on fixed stomachs. Basal gastric acid secretion was measured by the pyloric ligation method.The lesion index and maximum lesion depth did not differ in the BDL and sham groups, while they were significantly reduced in the CD group. Gastric acid output and secretory volume were reduced in the BDL group compared to the sham group, while these reductions were abolished in the CD group. Afferent denervation with capsaicin further reduced the ulcer index in the later group. Our data suggest that gastric mucosal susceptibility to injury is dependent on the normal flow of bile into the duodenal lumen, which appears to be a requirement for adaptive gastric cytoprotection., A. Cingi, R. Ahiskali, B. K. Oktar, M. A. Gülpinar, C. Yegen, B.Ç. Yegen., and Obsahuje bibliografii
The complex architecture of the liv er biliary network represents a structural prerequisite for the formation and secretion of bile as well as excretion of toxic substances through bile ducts. Disorders of the biliary tract affect a significant portion of the worldwide population, often leading to cholestatic liver diseases. Cholestatic liver disease is a condition that results from an impairment of bile formation or bile flow to the gallbladder and duodenum. Cholestasis leads to dramatic changes in biliary tree architecture, worsening liver disease and systemic illness. Recent studies show that the preva lence of cholestatic liver diseases is increasing. The availability of well characterized animal models, as well as development of visualization approaches constitutes a critical asset to develop novel pathogenetic concepts and new treatment strategies., L. Sarnova, M. Gregor., and Obsahuje bibliografii
Bilirubin is the final product of heme catabolism in the systemic circulation. For decades, increased serum/plasma bilirubin levels were considered an ominous sign of an underlying liver disease. However, data from recent years convincin gly suggest that mildly elevated bilirubin concentrations are as sociated with protection against various oxidative stress-mediated diseases, atherosclerotic conditions being the most clinically relevant. Although scarce data on beneficial effects of bilirubin had been published also in the past, it took until 1994 when the first clinical study demonstrated an increased risk of coronary heart disease in subjects with low serum bilirubin levels, and bilirubin was found to be a risk factor for atherosclerotic diseases independent of standard risk factors. Consistent with t hese results, we proved in our own studies, that subjects with mild elevation of serum levels of unconjugated bilirubin (benign hyperbilirubinemia, Gilbert syndrome) have much lower prevalence/incidence of cor onary heart as well as peripher al vascular disease. We have also demonstrated that this association is even more general, with serum bilirubin being a biomarker of numerous other diseases, often associated with increased risk of atherosclerosis. In addition, very recent data have demonst rated biological pathways modulated by bilirubin, which are responsible for observed strong clinical associations., L. Vítek., and Obsahuje bibliografii
A hydroponic experiment was conducted to investigate bioaccumulation and photosynthetic activity response to Cd in sweet sorghum seedlings. The seedlings were treated with 0, 50, and 100 μM Cd for 15 d. Our results showed that morphological characteristics of sweet sorghum were significantly affected by Cd treatments. The Cd concentrations in roots and shoots increased with increasing Cd concentrations in the nutrition solution; higher Cd accumulation was observed in the roots. Meanwhile, the photosynthetic activity decreased significantly and a shape of chlorophyll (Chl) a fluorescence transient in leaves was altered by Cd treatments. The Chl contents in the leaves decreased significantly, which was demonstrated by a change of spectral reflectance. Our data indicated that the higher Cd concentration reduced Chl contents and inhibited electron transport in the leaves, leading to the decrease of photosynthetic activity., Z. C. Xue, J. H. Li, D. S. Li, S. Z. Li, C. D. Jiang, L. A. Liu, S. Y. Wang, W. J. Kang., and Obsahuje bibliografii
From the bioarchaeological and morphofunctional point of view, a skeletal deformity is described from an individual buried in a settlement pit at an archaeological site Třebčín in Moravia dating from the period of the Great Moravian (AD 800-950). Macroscopic and radiological examinations are employed to diagnose pathological leasions on the skeleton of an adult female. Female was probably affected by a haematogenous form of acute osteomyelitis from early neonatal age., Anna Pankowská., and Obsahuje bibliografii
The BIOCEV project was launched October 31 at an official ceremony at the headquarters of the ASCR, with the inauguration of the first research program, Functional Genomics. BIOCEV is one of six major approved projects that are to become centers of scientific excellence in the Czech Republic and Europe. BIOCEV's research program is focused on two current and rapidly changing scientific fields - biomedicine and biotechnologies - and combines primary and applied research. The results of the scientific research are expected to be used to develop new medicaments and diagnostic processes. The new center is funded by the European Commission's Operational Program Research and Development for Innovation through a grant won by the Czech Academy of Sciences and Charles University Prague. and Luděk Svoboda.
Unikátní vakcína pro léčbu infekčních onemocnění, historicky první zdokumentování vývoje zubu, které může pomoci v boji proti rakovině, revoluční objev organismu bez mitochondrií neboli „buněčných elektráren“ -i takové jsou aktuální výsledky vědeckých týmů z centra BIOCEV, které za účasti významných hostů zahájilo 16. června 2016 plný provoz., A unique vaccine to treat infectious diseases, tooth development documented the first time in history that might help fight cancer and the revolutionary discovery of an organism without mitochondria, referred to as “cell power plants”. These are some of the current results from the BIOCEV research teams. The centre’s full operation was officially launched on June 16, 2016 in the presence of Czech and foreign guests from different areas of science and politics. The Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University in Vestec (BIOCEV) was established with substantial financial aid from the European Union. By 2020, 400 researchers and 200 master’s degree as well as postgraduate students are expected to work there. As of today, 56 research groups under five synergic research programmes are focused on obtaining more detailed understanding of organisms at the molecular level. Their results are oriented towards applied research and the development of new medical procedures to combat severe health problems., Isd., and Autor je podepsaný šifrou Isd.
Representatives of the Academy of Science of Czech Republic and Charles University have subscribed to the Founding Contract of the Biotechnological and Biomedical Center (BIOCEV) that will be situated in the vicinity of Vestec. and Luděk Svoboda.