Nedávno zesnulý významný český právní historik Jiří Kejř charakterizoval Vaněčkovo odborné dílo imponující rozsahem i hloubkou v roce 1985 slovy: „Od velikých monografií, kterým patří už právem označení klasické, a obsáhlých učebnic přes zevrubně fundované studie a články až k recenzím, drobným zprávám a veřejným projevům sahá jeho dílo o několika stech bibliografických položkách a ukazuje, jakou pílí a erudicí se vyznačoval jeho autor. Ne však množství publikací svědčí o místě učence ve vývoji vědy. […]“ and Hana Kábová.
Characterisation of proteases degrading ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO, EC: 4.1.1.39) was studied in the cowpea leaf during monocarpic senescence 3 and 9 d after flowering (DAF), representing early and mid pod fill. The stage at 3 DAF coincided with decrease in the metabolic parameters characterising senescence, i.e., contents of total soluble proteins, RuBPCO, and leaf nitrogen. At 9 DAF, there was a decline in total soluble proteins and an appearance of a 48 kDa cysteine protease. Characterisation of the proteases was done using specific inhibitors. Subcellular localisation at 3 DAF was studied by following the degradation of RuBPCO large subunit (LSU) in the vacuole lysates using immunoblot analyses. Cysteine proteases played a predominant role in the degradation of RuBPCO LSU at the crude extract level. At 9 DAF, expression of cysteine protease isoforms was monitored using polyclonal antibodies against papain and two polypeptides of molecular masses 48 and 35 kDa were observed in the vacuole lysates. We confirmed thus the predominance of cysteine proteases in the vacuoles during different stages of pod development in cowpea leaf. and B. Srivalli, Sudhakar Bharti, Renu Khanna-Chopra.
Inflammation and other immune responses are involved in the variety of diseases and disorders. The acute response to endotoxemia includes activation of innate immune mechanisms as well as changes in autonomic nervous activity. The autonomic nervous system and the inflammatory response are intimately linked and sympathetic and vagal nerves are thought to have anti-inflammation functions. The basic functional circuit between vagus nerve a nd inflammatory response was identified and the neuroimmunomodulation loop was called cholinergic anti-inflammatory pathway. Unique function of vagus nerve in the anti-inflammatory reflex arc was found in many experimental and pre-clinical studies. They br ought evidence on the cholinergic signaling interacting with systemic and local inflammation, particularly suppressing immune cells function. Pharmacological/electrical modulation of vagal activity suppressed TNF-α and other proinflammatory cytokines prod uction and had beneficial therapeutic effects. Many questions related to mapping, linking and targeting of vagal-immune interactions have been elucidated and brought understanding of its basic physiology and provided the initial support for development of Tracey's inflammatory reflex. This review summarizes and critically assesses the current knowledge defining cholinergic anti-inflammatory pathway with main focus on studies employing an experimental approach and emphasizes the potential of modulation of va gally-mediated anti-inflammatory pathway in the treatment strategies., I. Zila, D. Mokra, J. Kopincova, M. Kolomaznik, M. Javorka, A. Calkovska., and Obsahuje bibliografii
Reproductive medicine is one of the most progressive and most popular medicine branches. Its success and rapid development, together with the primacy of biological or genetic ties in the western construction of family is considered the main reason for decreasing popularity of adoption as the way of resolving involuntary childlessness. These assumptions are confronted with empirical findings about Czech population. The respondents of the survey conducted in the Czech households were asked about their preferences in the hypothetical situation of being confronted with physical infertility and about their attitudes towards various ways of solving it. The data showed that while infertility is actually constructed as a medical problem requiring high technology medical treatments, the adoption would not be considered a choice of last resort, after the failure of all procedures of artificial reproduction, including using donor gametes or embryo. Further, the data does not support the hypothesis of significant gender differences in these attitudes.