The Marsh fritillary (Euphydryas aurinia) (Lepidoptera: Nymphalidae) has declined across Europe, including the Czech Republic. Current conservation strategies rely on prevention of habitat loss and degradation, and increase in habitat quality and connectivity via promoting traditional grassland management. The population structure and adult demography parameters of a single population was investigated for eight years (single system), and of all the known Czech populations (multiple populations) for a single year, using mark-recapture. There was substantial variation in the patterns of adult demography, both among years in the single system and among the multiple populations in a single year. In the single system, the date of the first flight of an adult varied by 18 days over the 8 years and total annual numbers varied with a coefficient of variation of 0.40 (females fluctuating more than males). The average density was ca 80 adults/ha. The population size displayed density-dependence, i.e. decreased following years with high adult numbers, with an equilibrium density of 90 individuals/ha. The average density of the multiple populations was ca 120 individuals/ha. The estimated total population for the Czech Republic was 25,000 individuals (17,000 males / 8,000 females) in 2007, which does not indicate an imminent threat of extinction. The regional persistence of E. aurinia is likely to depend on re-colonisation of temporarily vacant sites by dispersing individuals, facilitated by local shifts in adult flight phenology to that better adapted to local conditions. and Kamil Zimmermann, Pavla Blazkova, Oldrich Cizek, Zdenek Fric, Vladimir Hula, Pavel Kepka, David Novotny, Irena Slamova, Martin Konvicka.
This note aims to put on record a hitherto unreported function of caterpillar setae supplementary to those already known. When woolly bear caterpillars of the moth Lemyra imparilis (Butler) have their body hairs removed and are dropped into a bucket of water from a height of 30 cm, they sink immediately. Caterpillars, however, with an intact coat of hairs usually float. This hitherto unreported role of caterpillar setae as life-rafts should depend on a high contact angle θe, and thus on the position, density and dimensions of the hairs. An SEM examination of the surface structure of the setae revealed a system of fine grooves and small distally pointing barbs on the hair shaft, which can trap air to support the weight of the insect. Insect setae generally and body hairs of caterpillars in particular are known to possess many functions, but a role as life-rafts for caterpillars in danger of drowning can now be added to the list of possible uses of body hairs., V. Benno Meyer-Rochow., and Obsahuje bibliografii
Euzophera pingüis (Lepidoptera: Pyralidae) is a secondary olive pest, which has increased in importance during recent years. In this study, more than 1300 predatory arthropods were collected from a Spanish olive orchard over two years and assayed using a pest-specific ELISA. Abundance and the percentage of positive responses to E. pingüis obtained using ELISA showed spiders to be the main predator, especially in 1998, when they accounted for 18% of the predation, followed by Scymnus suturalis (Coleoptera: Coccinellidae) and Brachinotocoris ferreri (Heteroptera: Miridae). Neuroptera and ants were less important as predators of E. pingüis.
While the ultimate causes and adaptive significance of sexual size dimorphism (SSD) have been extensively studied, the developmental mechanisms behind this phenomenon have received little attention. Going through an additional larval instar may form a specific way of achieving SSD in arthropods. In the present study, the mechanisms of SSD determination of two lymantriid moths, with marked SSD, were studied. In both species, females tended to go through an additional instar compared to males, and form pupae that were more than twice the weight of the males. To reveal the role of an extra instar, larval growth was monitored in the laboratory and the growth parameters were analysed as dependent on sex and developmental type (number of instars). Prolongation of growth by means of adding an additional larval instar in females turned out to be the key mechanism in the determination of the highly female-biased SSD in the species studied. There is thus a developmental mechanism available that permits achieving a larger size by means of extending the growth period. This provides evidence against constraint-based evolutionary explanations for body sizes in insects. There was no considerable accumulation of SSD during earlier larval life when females went through more instars than males. In contrast, in those cases in which males and females had the same number of instars, SSD accumulated gradually during the course of several larval instars. Longer growing period turned out to be a crucial mechanism leading to the female-biased SSD even when instar number did not differ between sexes, although higher instantaneous relative growth rates of females also played a complementary role in the latter case. Within sexes, an additional instar was characteristic of initially smaller larvae, as predicted by the "threshold size" hypothesis.
Microsatellites are the most common markers used in population and conservation genetic studies. However, their isolation is laborious and expensive. In some taxa, such as Lepidoptera, it is particularly difficult to isolate microsatellite markers due to the high similarity of the flanking regions of different loci and the presence of null alleles. Here we isolated microsatellites of the endangered butterfly Boloria aquilonaris using 454 GS-FLX Titanium pyro-sequences of biotin enriched DNA libraries and tested the success of cross-amplification on the sister-species B. eunomia. Fifteen polymorphic microsatellite loci were isolated in B. aquilonaris using initially 101 stringently designed primer pairs. Unlike in many other studies of microsatellite isolation in Lepidoptera, few null alleles were detected and only at very low frequencies. Additionally, the raw data set can still be used for the isolation of other microsatellite loci. None of the selected polymorphic loci for B. aquilonaris gave clear banding patterns for B. eunomia, although about 15 other loci gave promising banding patterns for the latter species. Low intra- and inter-specific transferability of developed markers in this study also lends support to the hypothesis that the evolution of the genome of Lepidoptera is dissimilar from that of other organisms., Sofie Vandewoestijne, Camille Turlure, Michel Baguette., and Obsahuje seznam literatury
The degree of polyandry in a species is linked to other life history traits such as egg maturation, life span, and male ejaculate size and quality. The study of differences in mating strategies between closely related species can provide a better understanding of the evolution of these strategies and of sperm competition. Mating patterns of two closely related species of small ermine moths (Yponomeuta) were investigated in the laboratory. The average female age at first mating was higher in Y. cagnagellus than in Y. padellus. Both species mated more than once; however, Y. cagnagellus females were more likely to remate in a short time frame. Moreover, Y. cagnagellus had higher life time female mating frequencies than Y. padellus (viz., 3.0 versus 2.0). These differences in mating frequency were confirmed in the field by examining the presence of spermatophores (or their remains) in the bursa as well as sperm in the spermatheca of field-caught females.
Some moth larvae feed not on plants but on keratin and/or chitin produced by animals. These substances are polymers and are commonly found in bird nests as feathers and raptor pellets. Many qualitative studies have examined the association of keratin/chitin feeding moths with bird nests. However, few studies have quantified the species composition with respect to type of nest and habitat. Hence, we have studied the degree to which the niches of these moths differ in terms of type of nest and habitat. We set-up open-top nest boxes for the Ural owl Strix uralensis (damp exposed nests from which owl chicks were fledged successfully) and artificial bird nests (mesh bags filled with duck down to imitate dry exposed nests) in a deciduous forest and artificial bird nests in an urban setting in Aichi Prefecture, central Japan, and collected the contents of the nests every two months from June to December 2014. We recorded 592 individuals of five keratin/chitin feeding moth species (Tineidae, Tineinae) from the contents. Using non-metric multidimensional scaling and cluster analysis of the relative abundances of individual species in each type of nest in forest and urban settings, these species were classified into three groups: (1) Monopis longella and M. congestella inhabiting forest, (2) M. flavidorsalis and Niditinea tugurialis mainly in damp exposed nests in forest and (3) N. piercella mainly in dry exposed nests in urban areas. This classification was compared with findings of other studies. As a result, these moths probably have different niches with respect to nest type (damp or dry), keratin/chitin as a source of food (raptor pellets or feathers), and habitat (forest or urban area). Furthermore, we suggest that the evolution of larviparity in M. congestella might be related to its preference for feathers as a source of food for the development of its larvae.
The use of light traps for controlling insect pests is restricted since they kill both pests and beneficial insects. It may be a possible to reduce the numbers of beneficial insects trapped by adjusting nightly trapping time based on differences recorded in the timing of the nocturnal flight peaks of target pests and beneficials. To test this, insects were collected hourly over night using black light traps at three locations in China from 2003 to 2005. Groups of lepidopteran and coleopteran pests were selected as the target pests that we would control by trapping and groups of beneficial predatory insects the catches of which needed to be reduced. The highest numbers of Coleoptera were caught between 20:00 and 22:00 h and of most Lepidoptera between 02:00 and 04:00 h. The hourly numbers of predatory insects caught by light traps were evenly distributed throughout the night. A model was developed to describe the relationships between the cumulative proportions of insects caught and time of night. The model accurately describes the flight activity of insects that were mainly caught before midnight, after midnight and evenly throughout a night by using different parameters for the three different insect groups. A beneficial-friendly trapping strategy was developed to reduce the numbers of beneficial insects trapped, which was based on differences in the nocturnal flight activity of pests and beneficial insects and validated by a field study in Shandong province. Results show that this trapping strategy reduced the number of beneficial insects caught by 46% and the electricity consumption by 50% compared to the traditional strategy. Thus this strategy is more beneficial-friendly than the traditional trapping strategy for controlling pests., Gang Ma, Chun-Sen Ma., and Obsahuje seznam literatury
A new species of Lymanopoda Westwood, a cloud forest Neotropical genus of Satyrinae, is described from the páramo grasslands on an isolated, peripheral massif in the Colombian Central Cordillera of the Andes: L. flammigera Pyrcz, Prieto & Boyer, sp. n. The genus Lymanopoda is species-rich (approx. 65 species) and its alpha taxonomy is relatively well researched. Relationships within the genus using molecular data have also been explored. The new species is outstanding for its golden yellow colour in males, not found in any other neotropical Satyrinae. Cladograms were constructed based on COI sequences of 47 species of Lymanopoda (~ 70% of the known species) including 17 from Colombia. The new species segregates in the "tolima" clade, which comprises four other high altitude Colombian species, as well as two from Ecuador. However, it is the comparative analysis of male genitalia, in particular the superuncus and valvae, which identified its closest relatives, thus confirming that genital characters can help refine molecular phylogenies. In addition to identifying species using mitochondrial DNA (mtDNA barcodes), nucleotide sites with unique fixed states used to identify nine species of Lymanopoda from Colombia are also presented., Tomasz W. Pyrcz, Carlos Prieto, Pierre Boyer, Jadwiga Lorenc-Brudecka., and Obsahuje bibliografii
Analysis of the mitochondrial DNA (mtDNA) control region (CR) was used to examine the dispersal of females of a geometrid moth, Epirrita autumnata, in Fennoscandia. A 542-bp-portion of the CR of 200 individuals from four northern and four southern localities was sequenced. The mtDNA CR of E. autumnata contains a substantial amount of variation as a total of 108 mtDNA haplotypes were observed. Between the northern and the southern localities (~1100 km), there was a moderate level of genetic differentiation (FST = 0.128). The amount of variation in the mtDNA CR of E. autumnata was lower in the north than in the south. The reduction in genetic variability may result from a combination of historical bottlenecks that date back to the post-glacial recolonization of Fennoscandia and, present-day bottlenecks due to the northern E. autumnata populations experiencing repeated outbreaks followed by collapse in population size. On a small spatial scale (0.6-19 km), within the northern and southern areas, no genetic differentiation was detected suggesting ongoing gene flow due to the dispersal of E. autumnata females among the localities. This finding was contrary to our earlier expectation of poor flying ability of E. autumnata females.