The response of some photosynthetic parameters (CO2 assimilation, transpiration rate, stomatal conductance, intercellular CO2 concentration, water-use efficiency, and chlorophyll content), shoot development, and the morphological features of the root system to differentiated conditions of nitrogen supply was tested in festulolium (Festulolium braunii K. Richert A. Camus) varieties (Felopa and Sulino). Nitrogen fertilization with no nitrogen added
[0 g(N)], single dosage [0.23 g(N)], and double dosage [0.46 g(N)] per pot and per year was applied. Lack of nitrogen resulted in formation of longer and finer roots and lowered chlorophyll content, CO₂ assimilation, and water-use efficiency, resulting in lower dry matter accumulation. Application of both dosages of nitrogen resulted in improved aboveground features, while root features were enhanced without nitrogen fertilization. Dependence between physiological parameters and morphological traits was significant and positively correlated in the case of the aboveground parts of plants and negatively correlated to the belowground parts., G. Mastalerczuk, B. Borawska-Jarmułowicz, H. M. Kalaji, P. Dąbrowski, J. Paderewski., and Obsahuje bibliografii
We studied water relations and gas exchange in six almond genotypes grafted on GF677 in response to withholding irrigation for 14 days and a subsequent 10-day rehydration period. The responses to drought stress significantly differed in the almond genotypes; the tolerant plants were distinguished and monitored. Leaf relative water content (RWC) decreased by more than 23%, leaf water potential dropped to less than -4.3 MPa, and electrolyte leakage increased to 43% in dehydration-sensitive genotypes. Photosynthesis (PN) and stomatal conductance (gs) of drought-sensitive genotypes were significantly reduced by 70% and 97% in response to water deficiency. Water stress significantly enhanced wateruse efficiency up to 10 folds in drought-tolerant almonds. The difference between leaf temperature and its surrounding air temperature (ΔT) increased significantly to more than 187% under water stress in drought-tolerant genotypes. In addition, the reduction in the g s and further ability to preserve RWC were involved probably in drought-tolerance mechanism in almond. Negative significant correlations were found between ΔT, PN, and gs. Based on the correlations, we suggested that ΔT could be used as a simple measurement for monitoring water stress development in the irrigation management of almond orchards. In conclusion, ‘Supernova’ and the Iranian genotypes ‘6-8’ and ‘B-124’, were found to be more droughttolerant compared with other genotypes in this experiment., S. Karimi, A. Yadollahi, K. Arzani, A. Imani, M. Aghaalikhani., and Obsahuje bibliografii
In this work, photosystem II (PSII) photochemistry, leaf water potential, and pigment contents of male and female Pistacia lentiscus L. were investigated during a seasonal cycle at three different, arid locations: superior semiarid, inferior semiarid, and arid. The results showed that the gender, season, and the site conditions interacted to influence the quantum yield and pigment contents in P. lentiscus. Predawn leaf water status was determined only by the site and season. The annual patterns of PSII maximum quantum efficiency (Fv/Fm) were characterized by a suboptimal activity during the winter, especially, populations with the more negative water potential exhibited a lower chlorophyll (Chl) a content and chronic photoinhibition irrespective of a gender. We also demonstrated that both photochemical or nonphotochemical mechanisms were involved to avoid the photoinhibition and both of them depended on the season. This plasticity of photosynthetic machinery was accompanied by changes in carotenoids and Chl balance. In the spring, the female Fv/Fm ratio was significantly higher than in male individuals, when the sexual dimorphism occurred during the fruiting stage, regardless of site conditions. P. lentiscus sex-ratio in Mediterranean areas, where precipitations exceeded 500 mm, was potentially female-biased. Among the fluorescence parameters investigated, nonphotochemical quenching coefficient appeared as the most useful one and a correlation was found between Chl a content and Fv/Fm. These results suggest that functional ecology studies would be possible on a large scale through light reflectance analysis. and S. Ait Said ... [et al.].
Genes differentially expressed under high irradiance (HI) stress in mature flag leaves of super-hybrid rice Liangyoupeijiu (Oryza sativa ssp. indica) were studied by the silver-staining mRNA differential display technique. We obtained 167 differentially displayed bands on silver-stained polyacrylamide gels and searched for their genetic origins. Five cDNA fragments, which were upregulated by HI stress, were cloned and sequenced. The clones of G25×320, A31×380, and G29×280 putatively encode a cytosolic monodehydroascorbate reductase (MDAR), a phosphatidylinositol 4-kinase (PI-4-K), and a DEAH-box RNA helicase, respectively. Most differentially expressed genes in hybrid rice were inherited from parents and many of them were related with both parents, although some were derived from one parent only. The differentially expressed cDNA fragments having no distinct genetic origins indicated the involvement of some unclear mechanisms in the inheritance processes from parents to hybrid. and G.-Q. Jiang ... [et al.].
In Rhodobacter capsulatus, two triple mutants were constructed. In these non-photosynthetic mutants, two amino acids near the quinone QB have been mutated to two alanines: in the QA site have been mutated to alanine-aspartic acid and glutamic acid-alanine. Several spontaneous mutants derived from original constructs were selected. DNA sequencing experiments on originally designed mutant strains and their spontaneous mutants were performed to identify possible genetic reversions at quinone site-specific locations. Constructed mutants carry double alanines in the QB site and single alanine in the QA site. Spontaneous mutants carry additional compensating mutations, aspartic acid (L225), cysteine (M231), and serine (M231) far from QA and QB sites, which may be involved in quinone binding by the photosynthetic reaction centres.
The genetic basis of stomatal conductance (gs), net photosynthetic rate (PN), and transpiration rate (E) was explored by using a wheat doubled haploid population from a cross of Hanxuan10 and Lumai 14. The above three traits were evaluated in wheat flag leaves at 10, 20, 30 days after anthesis under drought stress (DS) and well-watered (WW), and quantitative trait loci (QTL) were analyzed. Expression of the traits during the grain filling stage showed downward trends under both conditions, but expression of three phenotypes were stronger under WW than those under DS. Extremely significant positive correlations were established among the traits at all growth stages under both conditions. A total of 18 additive QTLs for those traits were identified on 10 chromosomes. Among them, two batches of nine additive QTLs were associated with the target traits under DS and WW, respectively. Two additive QTLs for gs and E, two for gs and PN, six for gs, PN, and E clustered at the same or near the region (colocation) of chromosomes 4A, 2B, and 7B, respectively. This provided genetic basis for close phenotype correlations among gs, PN, and E. Furthermore, QTLs for gs, PN, and E near Xgwm577 and Xgwm611 located on 7B chromosome were linked to previously reported QTLs regulating a SPAD value and the chlorophyll a/b ratio under dark-induced condition. This finding indicated that these QTLs on 7B chromosome might be involved in the process of wheat leaf senescence., S. G. Wang, S. S. Jia, D. Z. Sun, H. Y. Wang, F. F. Dong, H. X. Ma, R. L. Jing, G. Ma., and Obsahuje bibliografii
Photochemical activity of isolated mesophyll chloroplasts was measured as Hill reaction activity (HRA) and photosystem 1 (PS1) activity in three diallel crosses of maize (Zea mays L.) inbred lines and F1 hybrids. Statistically significant differences between genotypes together with positive heterotic effect in F1 generation were found for both traits studied. These differences were more pronounced when HRA or PS1 activity was expressed per leaf area unit or dry matter unit compared to the expression per chlorophyll content unit. Analysis of variance showed that both the genetic and non-genetic components of variation in the photochemical activity of isolated mesophyll chloroplasts are present in all three diallel crosses examined. The positive heterosis in F1 hybrids probably arises from non-additive genetic effects of a positive dominance type. Additive genetic effects were also statistically highly significant. We found no differences between reciprocal crosses. and D. Holá ... [et al.].
High variability in leaf gas exchange and related traits were found in 30 genotypes of field grown finger millet. The variability in carbon exchange rate per unit leaf area (PN) can be partly attributed to the differences in the stomatal conductance (gs) and area leaf mass (ALM). The PN was positively correlated with total dry matter (TDM). However, no relationship between PN and seed yield was found. The leaf area showed a positive and significant correlation with total biomass. None of the other gas-exchange traits had significant relationship either with TDM or with seed yield. The ALM showed a strong positive association with PN. However, it was not correlated with either total biomass or seed yield. As a result, the use of ALM as surrogate for PN for identifying high biomass producing genotypes only had a limited value. Hence selection for high PN would result in higher biomass producing types.
In a field rain-fed trial with 15 cassava cultivars, leaf gas exchanges and carbon isotope discrimination (Δ) of the same leaves were determined to evaluate genotypic and within-canopy variations in these parameters. From 3 to 7 months after planting leaf gas exchange was measured on attached leaves from upper, middle, and lower canopy layers. All gas exchange parameters varied significantly among cultivars as well as canopy layers. Net photosynthetic rate (PN) decreased from top canopy to bottom indicating both shade and leaf age effects. The same trend, but in reverse, was found with respect to Δ, with the highest values in low canopy level and the lowest in upper canopy. There were very significant correlations, with moderate and low values, among almost all these parameters, with PN negatively associated with intercellular CO2 concentration (Ci), ratio of C i to ambient CO2 concentration C i/C a, and Δ. Across all measured leaves, Δ correlated negatively with leaf water use efficiency (WUE = photosynthesis/stomatal conductance, gs) and with gs, but positively with Ci and Ci/Ca. The later parameters negatively correlated with leaf WUE. Across cultivars, both PN and correlated positively with storage root yield. These results are in agreement with trends predicted by the carbon isotope discrimination model. and M. A. El-Sharkawy, S. M. de Tafur.
The genetic variation in low temperature sensitivity of eight tomato genotypes grown at suboptimal temperature (19 °C) and at low irradiance (140 pmol m'2 s**) was assessed at the plant, chloroplast and thylakoid membrane levels. Temperature effects on the thylakoid membrane were determined by measuring the maximum fluorescence (Fp) and the maximal fluorescence rise (ADP) of induction traces of leaf discs at decreasing temperatures (30, 28, ... 0 °C). Two discontinuities were found in Fp versus temperature curves: a low temperature break at ca. 12 °C (LTB) and a high temperature break at ca. 22 °C (FITB). Below LTB, sFp and sDP were determined as the temperature induced changes in Fp, respectively ADP. Chloroplast functioning was determined by measuring net CO2 fixation rate (E^) of leaves. Plant performance was determined by measuring the increase in leaf area and sho ot dry mass in time. Correlations between the various parameters were analysed across the genotypic variation found. Chlorophyll (Chl) fluorescence parameters were not correlated with plant performance at suboptimal growth conditions. of leaves was correlated with plant performance, but only at ambient CO2. Effects of stomatal resistance on were large. The Chl fluorescence parameters LTB, sFp and sDP could distinguish between tomato genotypes. Nevertheless, the ranking of the genotypes depended on the specific parameter selected, indicating that each parameter assessed a different aspect of the heterogeneous temperature dependence of Chl fluorescence induction. Their genetic variation suggested that the genotypes differed in the organisation and fimctioning of the thylakoid membrane. These differences were not reflected in of leaves or plant performance.