Typical chestnut thylakoid extracts isolated by mechanical disruption of leaf tissues had an equivalent of 0.28 kg m-3 chlorophyll (Chl) which is six times less than in thylakoids obtained from spinach, although Chl content in leaves was only half as small. According to optical microscopy, the vesicles showed a good integrity, exhibiting at 21 °C a high capacity of photon-induced potential membrane generation, which was demonstrated by the almost full 9-amino-6-chloro-2-methoxyacridine fluorescence quenching in a hyper-saline medium containing 150 mM KCl and having osmotic potential of -1.5 MPa. The half-time of the thylakoid potential generation was 11.7 s with the time of dissipation around 8.9 s. In such conditions, spinach thylakoids showed an increased swelling and also differences in the half-time generation which was almost four times faster than was observed in chestnut. However, when spinach thylakoids were incubated in a typical hypo-saline medium without KCl with osmotic potential -0.8 MPa, no additional swelling was observed. Consequently the half-time of potential dissipation was 35 s. Studies with nigericin suggested a chestnut thylakoid ΔpH significantly smaller than that observed in spinach, which was confirmed by the measurements of the ATP driven pumping activity. and J. Gomes-Laranjo ... [et al.].
A strain of Synechocystis sp. PCC 6803 expressing the yellow fluorescent protein (YFP) fused to the C-terminus of the PsaF subunit of PSI has been constructed and used to isolate native PSI complexes employing the GFP-Trap®, an efficient immunoprecipitation system which recognizes the green fluorescent protein (GFP) and its variants. The protein analysis and spectroscopic characterization of the preparation revealed an isolate of trimeric and monomeric PSI complexes, which showed minimal unspecific contamination as demonstrated by comparison with the wild type control. Interestingly, we detected CP43 subunits of PSII and small amounts of PSII core complexes specifically pulled-down with the YFP-PSI, supporting the association of PSII assembly modules and intermediate assembly complexes with PSI, as observed in our previous studies. The results demonstrate that the GFP-Trap® system represents an excellent tool for studies of PSI biogenesis and interconnection of PSI and PSII assembly processes., A. Strašková, J. Knoppová, J. Komenda., and Obsahuje bibliografické odkazy
Canonic law of the late middle ages considered the participation of the clerics in killing rather strictly. The Lateran Council 1215 established that a cleric was neither allowed to issue or declare a sentence of death nor to draft or write papers in connnection with it. The edition of the Apostolic Penitentiary Supplications Registers enables people to judge the situation in Bohemia in the period from the 1430´s to the end of the same century. In the examined period a very specifi c case of „complicity in killing“ appeared four times – a phenomenon connected with reading and writing knowledge peculiar to clerics. They read written orders and wrote for the needs of fighting sides. The argumentation is similar in all cases. Th e matter was always activity on command, resulting moreover from the specific condition of the person in question (the only literate, servant). He never participated in the fight actually. With regard to the volume of analogous scribe activity which can be supposed in the period in question, these four cases were certainly just a small fragment of the actual participation of clerics. The situation in Bohemia is doubtlessly specific because of the fact that utraquist disciples and clerics who wrote in the hussite services of course didn´t appeal to the Penitentiary. Nevertheless it must be supposed that the Penitentiary solved similar cases even in the Czech catholic environment only exceptionally.
Canonic law of the late middle ages considered the participation of the clerics in killing rather strictly. The Lateran Council 1215 established that a cleric was neither allowed to issue or declare a sentence of death nor to draft or write papers in connnection with it. The edition of the Apostolic Penitentiary Supplications Registers enables people to judge the situation in Bohemia in the period from the 1430´s to the end of the same century. In the examined period a very specifi c case of „complicity in killing“ appeared four times – a phenomenon connected with reading and writing knowledge peculiar to clerics. They read written orders and wrote for the needs of fighting sides. The argumentation is similar in all cases. Th e matter was always activity on command, resulting moreover from the specific condition of the person in question (the only literate, servant). He never participated in the fight actually. With regard to the volume of analogous scribe activity which can be supposed in the period in question, these four cases were certainly just a small fragment of the actual participation of clerics. The situation in Bohemia is doubtlessly specific because of the fact that utraquist disciples and clerics who wrote in the hussite services of course didn´t appeal to the Penitentiary. Nevertheless it must be supposed that the Penitentiary solved similar cases even in the Czech catholic environment only exceptionally.
Canonic law of the late middle ages considered the participation of the clerics in killing rather strictly. The Lateran Council 1215 established that a cleric was neither allowed to issue or declare a sentence of death nor to draft or write papers in connnection with it. The edition of the Apostolic Penitentiary Supplications Registers enables people to judge the situation in Bohemia in the period from the 1430´s to the end of the same century. In the examined period a very specifi c case of „complicity in killing“ appeared four times – a phenomenon connected with reading and writing knowledge peculiar to clerics. They read written orders and wrote for the needs of fighting sides. The argumentation is similar in all cases. Th e matter was always activity on command, resulting moreover from the specific condition of the person in question (the only literate, servant). He never participated in the fight actually. With regard to the volume of analogous scribe activity which can be supposed in the period in question, these four cases were certainly just a small fragment of the actual participation of clerics. The situation in Bohemia is doubtlessly specific because of the fact that utraquist disciples and clerics who wrote in the hussite services of course didn´t appeal to the Penitentiary. Nevertheless it must be supposed that the Penitentiary solved similar cases even in the Czech catholic environment only exceptionally.