In spring and winter cultivars of oilseed rape (Brassica napus var. oleifera), acclimation of photosynthetic apparatus to cold was connected with the increase in activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) and sucrose-phosphate synthase (SPS). Conversely, cold de-acclimation entailed the decline of RuBPCO and SPS activities. The rate of this photosynthetic de-acclimation might depend on day temperature. On the other hand, temperature rise during de-acclimation (identical during the day and night) resulted in the improvement of photosynthetic activity measured by means of chlorophyll fluorescence. An increase in SPS activity (and even transitory increase in RuBPCO activity) was observed when the elongation growth rate (EGR) accelerated during de-acclimation. Throughout re-acclimation, plants with high EGR were unable to maintain or recover higher photosynthetic capacity, despite the fact that SPS activity remained high or even increased during re-acclimation. and M. Rapacz, K. Hura.
The cytochrome b6f (Cyt b6f) complex, which functions as a plastoquinol-plastocyanin oxidoreductase and mediates the linear electron flow between photosystem II (PSII) and photosystem I (PSI) and the cyclic electron flow around PSI, was isolated from spinach (Spinacia oleracea L.) chloroplasts using n-octyl-β-D-glucopyranoside (β-OG). The preparation was also able to catalyze the peroxidase-like reaction in the presence of hydrogen peroxide (H2O2) and guaiacol. The optimal conditions for peroxidase activity of the preparation included: pH 3.6, ionic strength 0.1, and temperature 35°C. The apparent Michaelis constant (Km) values for H2O2 and guaiacol were 50 mM and 2 mM, respectively. The bimolecular rate constant (k obs) was about 26 M-1 s-1 and the turnover number (K cat) was about 60 min-1 (20 mM guaiacol, 100 mM sodium phosphate, pH 3.6, 25°C, [H2O2]<100mM). These parameters were similar to those of several other heme-containing proteins, such as myoglobin and Cyt c. and X. B. Chen ... [et al.].
Kandelia candel (L.) Druce is the dominant mangrove species on the west coast of northern Taiwan. We have measured the net photosynthetic rate (PN) and chlorophyll (Chl) a fluorescence of seedlings grown at combinations of two nitrogen (0.01 and 0.1 mM) and two NaCl (250 and 430 mM NaCl) controls. With the same nitrogen level, seedlings grown at higher salinity (HS) had a significantly lower PN and stomatal conductance (gs) than those at lower salinity (LS). An increase in nitrogen availability significantly elevated PN and gs of the LS-grown seedlings. Compared to dark adapted leaves, the maximum quantum yield of photosystem 2 (PS2) (Fv/Fm) of leaves exposed to PFDs of 1200 and 1600 µmol m-2 s-1 for 2 h was significantly reduced. The degree of Fv/Fm reduction differed among leaves of the four types of treated plants. Chl fluorescence quenching analysis revealed differences among the examined plants in coefficients of non-photochemical and photochemical quenching. and Wen-Yuan Kao, Hung-Chieh Tsai.
Foliar gas exchange characteristics, understorey microclimate, and crown irradiation were assessed for saplings of eight canopy tree species in two plots of neotropical rain forest with different degrees of canopy opening. Species studied belonged to different putative guilds: shade intolerants (both short-lived--pioneers--and long-lived), intermediates, and shade-tolerants. A considerable overlap was recorded between species in values of the photosynthetic rate per unit leaf area (PN). The highest median PN (1.26 µmol m-2 s-1) was recorded in the pioneer Croton killipianus, while slightly lower median values were recorded in Simarouba amara and Pentaclethra macroloba, and markedly lower values in two species of Vochysiaceae (Qualea paraense and Vochysia ferruginea), both putative intolerants. Highest median stomatal conductance (gs) was also shown by C. killipianus, while S. amara, P. macroloba, and L. procera exhibited intermediate values, and the lowest gs was shown by V. ferruginea and Q. paraense. Overall irradiance and crown irradiation, PN, and gs of saplings were higher in the plot which had previously received a silvicultural treatment. Most values of photosynthetic photon flux density (PPFD) were <100 µmol m-2 s-1 in both plots, with shortlived peaks of up to 2000 µmol m-2 s-1 in the treated plot. When the relationship between PN and irradiance (I) was examined by fitting PN/I curves, the degree of fit varied markedly between species, values of the regression coefficient r2 were between 0.09 and 0.51. No significant differences between species were recorded in Pmax and species also demonstrated little variation in the predicted values of dark respiration (RD), values varying between -0.51 and -1.46 µmol m-2 s-1 in Q. paraense and Minquartia guianensis, respectively. Fitted values of apparent quantum efficiency were also fairly uniform, generally falling within the range 0.02-0.03 mol(CO2) mol-1(photon). and N. E. Vera, B. Finegan, A. C. Newton.
At the vegetative growth stage (40 d), the mean photosynthetic rate (PN) and canopy photosynthesis (PN × LAI) in F1 hybrids and their parents were similar, whereas the maintenance respiration rate (RM) was considerably higher and PN/RM lower in the F1 hybrids than in the parents. Yet at the flowering stage, the hybrids showed higher PN and PN × LAI values, while RM and PN/RM were similar in both. A specific F1 hybrid like IR 62829A×Vajram showed high readings in PN, PN/RM, and PN × LAI at the flowering stage, while IR 62829A×Swarna followed by IR 62829A×Vajram had high values 40 d after planting. The parents Swarna and Vajram, although moderate in PN, had the highest PN × LAI at the flowering stage due to a greater LAI. and M. J. Baig, P. Swain, K. S. Murty.
The photosynthetic pathway of the roots (both the white velamentous main portions and the green, nonvelamentous tips) was investigated in twelve taxa (natural species and intergeneric hybrid cultivars) of epiphytic orchids having CAM leaves. All organs contained chlorophyll, and the a/b ratios indicate that the organs, especially the roots, are likely shade-adapted. Stable carbon isotope ratios of the tissues were near -15‰ for all organs, a value typical of obligate (constitutive) CAM plants. Values for root tissues were slightly lower (more negative) than those of the leaves. The presence of CAM in the leaves of these orchids did not ensure that their roots performed CAM photosynthesis. Further work is needed to address the questions raised in this study and to determine if the photosynthetic roots of these taxa are capable of assimilating atmospheric CO2. and C. E. Martin ... [et al.].
Field measurements of gas exchange were made using a portable infra-red gas analyser on six species of early-successional woody plants in Singapore. Macaranga heynei, Mallotus paniculatus and Trema tomentosa grow on relatively fertile soils. Adinandra dumosa, Uillenia suffruticosa and Melostoma malabalhricum are species typical of extremely acidic and infertile soils. The six species were similar in their photosynthetic responses to irradiance when net photosynthetic rate (P^) was expressed on a leaf area basis. However, when PN was converted to a rate per unit leaf dry mass, the fertile soil species showed higher rates of dark respiration and photosynthesis. No difference in water use efficiency between the two sets of species was found.
We studied the photosynthetic performance of sterile and fertile sporophytes in a natural population of the fern Dryopteris affinis growing within a riparian forest (Central Italy) using chlorophyll (Chl) a fluorescence transients, the OJIP phase, where O is for the minimum fluorescence, P is for the peak (the maximum), and J and I are inflections. The “vitality” of the samples was assessed by the maximum quantum yield of primary photochemistry obtained indirectly from the fluorescence data (Fv/Fm); in the same way, the so-called performance index (PIABS) was obtained from fluorescence data. The photosynthetic performance (inferred from PIABS) of D. affinis changed significantly with the seasonal development of the fronds. The highest photosynthetic performance was recorded in the summer, corresponding to the period of spore release. The photosynthetic performance decreased in the winter, down to the minimal values of senescent fronds reached at the end of the seasonal cycle (May-June). On the whole, during the seasonal development, sterile and fertile fronds had a similar photosynthetic behaviour, as inferred from fluorescence data. At the end of spore maturation and dispersal (September-October), the fertile fronds showed somewhat lower photosynthetic performance than the sterile fronds, as revealed by PIABS. Being a long-lived fern, confined to humid and undisturbed sites in the Mediterranean, D. affinis deserves to be further investigated as a potential indicator of ecological continuity in Mediterranean riparian forests., L. Paoli, M. Landi., and Obsahuje bibliografii
The effects of a high temperature on leaf photosynthetic response of Lupinus albus, a species native from the Mediterranean zone, were studied under varying tissue water status and irradiance (/). At leaf temperatures optimal (25 °C) or near-optimal (15 ®C) for carbon assimilation in lupins, photosynthetic capacity (P^) was decreased at leď relative water content (RWC) around 60 %. The temperature above the optimum (e g. 35 oC) decreased at high RWC (80 %). Irrespective of the leaf water status the optimum leaf temperature for in lupins was around 25 "C. The inhibition of P^ by lupin leaf discs at 35 °C was greatly enhanced at high I. Leaf disc treatment at 35 in the dark did not change quantum yield or Pn. When in addition to 35 the leaves were subjected to high I (TS + IS) or to high I and dehydration (TS + IS + WS), then a substantial decrease was observed in the quantum yield (66 and 77 % of the values in Controls, respectively) and in (31 and 56 %, respectively).
Plants of Solanum curtilobum (from high altitude) and Solanum tuberosum (from low altitude) were grown in open-top chambers in a greenhouse at either ambient (AC, 360 µmol mol-1) or ca. twice ambient (EC, 720 µmol mol-1) CO2 concentrations for 30 d. CO2 treatments started at the reproductive stage of the plants. There were similar patterns in the physiological response to CO2 enrichment in the two species. Stomatal conductance was reduced by 59 % in S. tuberosum and by 55 % in S. curtilobum, but such a reduction did not limit the net photosynthetic rate (PN), which was increased by approximately 56 % in S. curtilobum and 53 % in S. tuberosum. The transpiration rate was reduced by 16 % in both potato species while instantaneous transpiration efficiency increased by 80 % in S. tuberosum and 90 % in S. curtilobum. Plants grown under EC showed 36 and 66 % increment in total dry biomass, whereas yields (dry mass of tubers) were increased by 40 and 85 % in S. tuberosum and S. curtilobum, respectively. EC promoted productivity by increasing PN. Thus S. tuberosum, cultivated around the world at low altitudes, and S. curtilobum, endemic of the highland Andes, respond positively to EC during the tuberisation stage. and N. Olivo, C. A. Martinez, M. A. Oliva.