The oxidizing side of photosystem 2 (PS2) contains a set of unique redox components including two tyrosine radicals, Tyr2 and Tyrp, and a cluster of 4 Mn atoms that are involved in the oxidation of water to molecular oxygen. The structural environment of these components is unknown; we ušed computer-assisted modelling to create a three-dimensional model for the structure around Tyr^ and Tyrj). The tyrosines are predicted to be located in hydrophobic cavities in the Dl and D2 reaction centre proteins, respectively. The cavities are situated close to the lumenal surface of the thylakoid membrane and are surrounded mainly by highly conserved amino acid residues. Both tyrosines are proposed to be hydrogen-bonded to the nearby histidine residues Hisl90 on respective protein. We tested the model by electron paramagnetic resonance (EPR) spectroscopy of Tyr^ and site-directed mutagenesis of the D2 protein in the cyanobacterium Synechocystis 6803. In two mutants, where the corresponding histidine is changed to a tyrosine or a leucine, the normál EPR spectra from Tyr^ were drastically altered to narrow structureless radical signals with g-values similar to that of Tyrp (g » 2.0050). The new spectra were assigned to Tyrp from functional experiments and the spectral modification indicated that the introduced point-mutation of His 190 (D2-Hisl89 in Synechocystis 6803) modified the environment around Tyr^ supporting the structural model. In a third mutant where Glnl65 (D2-Glnl64 in Synechocystis 6803) was exchanged to a leucine we also observed a modified EPR spectrum consistent with the predictions firom the model. AU the experimental data obtained strengthen the Computer derived model in essential aspects. We also predicted a possible location for the Mn-cluster to the loop that connects the membrane spanning helices A and B on the Dl protein. In addition the structural model suggests the presence of a metal-site, possibly a Mn site, close to Tyrz on the Dl protein. The site is proposed to be constituted of three residues on the Dl protein, Glnl65, Aspl70 and Glul89.
Phoridae (scuttle flies) are widely distributed, occur in many types of habitats and are ecologically versatile, which makes them an excellent bioindicator group for evaluating faunal diversity. The structure of scuttle fly communities was compared in two Mediterranean habitats in the Montseny Natural Park (Catalonia, Spain) that differ in vegetation and microclimate: beech forest and highland scrubland. 3684 male individuals belonging to 135 species of scuttle flies were identified. Scuttle flies were more abundant in beech forest than scrubland. Observed and estimated species richness were lower in scrubland than in beech forest, while diversity was similar in both habitats. Community evenness was greater in scrubland than beech forest. Therefore, the percentage of dominant and subdominant species was higher in scrubland than beech forest, while the percentage of rare species was higher in beech forest than scrubland. Scuttle fly species composition was significantly different in the two habitats, but it was similar among plots within the same habitat. Megaselia pectoralis (Wood, 1910) and Megaselia subpleuralis (Wood, 1909) were the dominant species in beech forest, while Megaselia pusilla (Meigen, 1830), Megaselia pumila (Meigen, 1830), Megaselia superciliata (Wood, 1910) and Megaselia diversa (Wood, 1909) were the dominant species in scrubland. Trophic specialization was higher in beech forest than scrubland. Saprophages were the dominant trophic group in beech forest, while fungivores and polyphages were dominant in scrubland. The high biodiversity of scuttle flies recorded in the Montseny Natural Park indicates that there is also a high diversity of other taxa there and that these Mediterranean mountains are of high conservation status., Carlos García-Romera, José A. Barrientos., and Obsahuje bibliografii
Scanning electron microscopy of the cystidicolid nematode Ascarophis mexicana Moravec, Salgado-Maldonado et Vivas-Rodríguez, 1995 enabled the first detailed study of its cephalic structure. In contrast to most Ascarophis species, its pseudolabia are highly reduced and sublabia are unlobed and weakly developed. Similascarophis is considered a synonym of Ascarophis, to which two its species are transferred as A. maulensis (Muñoz, González et George-Nascimento, 2004) comb. n. and A. chilensis (Muñoz, González et George-Nascimento, 2004) comb. n.
Scanning electron microscopy (SEM) examination of a paratype specimen (gravid female) of the philometrid nematode Paraphilometroides nemipteri Moravec et Shaharom-Harrison, 1989, the type species of Paraphilometroides Moravec et Shaharom-Harrison, 1989, made it possible to recognise the true structure of the cephalic end and the shape of cuticular ornamentations on the body. In contrast to other philometrid genera, the cephalic end is angular in lateral view and rounded in dorsoventral view; large external cephalic papillae are fused together to form a marked dorsal and a ventral ala, each bent into a curve; six minute internal papillae, including a pair of previously not reported lateral papillae, were found; amphids were indistinct. Cuticular ornamentations (inflations) were found to be transversely elongated. These features, especially the unique cephalic structure, clearly distinguish Paraphilometroides from other philometrids and confirm the validity of this genus.
The structure of the vertebrate predator community in north-eastern Belarus has been examined before and after naturalization of the American mink and raccoon dog. Species composition of the community, population density and biomass, portion of each species in the pooled community density and biomass, species body mass and size structure of the community were investigated. The community consisted of 36 native predator species, and 11 other predator species were sporadically registered in the area in both periods before the American mink and raccoon dog expansion and after the introduced species have reached a high population density. Separating predator species into four size groups, we found that in terms of total density the largest portion of the community belonged to small predators, while large predators formed the smallest portion. In terms of total biomass, the larger medium-sized predator group predominated over other size groups. Despite decline in the populations of ten native predator species, the total community density and biomass as well as portions of different size groups appeared to be very similar before and after naturalization of the introduced predator species.
NMDA receptors have received much attention over the last few decades, due to their role in many types of neural plasticity on the one hand, and their involvement in excitotoxicity on the other hand. There is great interest in developing clinically relevant NMDA receptor antagonists that would block excitotoxic NMDA receptor activation, without interfering with NMDA receptor function needed for normal synaptic transmission and plasticity. This review summarizes current understanding of the structure of NMDA receptors and the mechanisms of NMDA receptor activation and modulation, with special attention given to data describing the properties of various types of NMDA receptor inhibition. Our recent analyses point to certain neurosteroids as NMDA receptor inhibitors with desirable properties. Specifically, these compounds show use-dependent but voltage-independent block, that is predicted to preferentially target excessive tonic NMDA receptor activation. Importantly, neurosteroids are also characterized by use-independent unblock, compatible with minimal disruption of normal synaptic transmission. Thus, neurosteroids are a promising class of NMDA receptor modulators that may lead to the development of neuroprotective drugs with optimal therapeutic profiles., V. Vyklicky ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
We studied the development of chloroplasts from etioplasts in the cotyledonary leaves of 4-d-old dark-grown cucumber (Cucumis sativus) seedlings after irradiation (20 μmol m-2 s-1). Upon irradiation, the triggering of chlorophyll (Chl) synthesis and accumulation showed a relatively short lag phase. The irradiation of etiolated seedlings initiated the synthesis of apoproteins of pigment-protein complexes. While Chl-protein 2 (CP2) was detected at 6 h after irradiation, CP1 only after 29 h. The appearance and accumulation of some of the apoproteins were monitored by Western-blotting. LHC2 apoprotein was detected after a 6 h-irradiation. The amounts of D1 protein of photosystem (PS) 2 and PsaA/B protein of PS1 were quantitated by ELISA. Further, the thylakoid membrane function during this time period in terms of PS1- and PS2-mediated electron transfer activity and intersystem electron pool size were analyzed. While PS1 activity was detected after 4 h, PS2-mediated O2 evolution was detected only after a 17 h-irradiation. Fv/Fm value of Chl a fluorescence measurements indicated that the photochemical efficiency of these leaves reached its maximum after 29 h of irradiation. The intersystem pool size of cotyledonary leaves was equivalent to that of the control cotyledonary leaves grown for 25 h under continuous irradiation. Thus etioplasts develop into fully functional chloroplasts after approximately 25 h when 4 d-dark grown cucumber seedlings are continuously moderately irradiated. The development of photosynthetic electron transport chain seems to be limited in time at the level of PS2, possibly at the donor side. and K. Bala Krishna ... [et al.].
Sarcoplasmic reticulum (SR) is a specialized tubular network, which not only maintains the intracellular concentration of Ca2+ at a low level but is also known to release and accumulate Ca2+ for the occurrence of cardiac contraction and relaxation, respectively. This subcellular organelle is composed of several phospholipids and different Ca2+-cycling, Ca2+-binding and regulatory proteins, which work in a coordinated manner to determine its function in cardiomyocytes. Some of the major proteins in the cardiac SR membrane include Ca2+-pump ATPase (SERCA2), Ca2+-release protein (ryanodine receptor), calsequestrin (Ca2+-binding protein) and phospholamban (regulatory protein). The phosphorylation of SR Ca2+-cycling proteins by protein kinase A or Ca2+-calmodulin kinase (directly or indirectly) has been demonstrated to augment SR Ca2+-release and Ca2+-uptake activities and promote cardiac contraction and relaxation functions. The activation of phospholipases and proteases as well as changes in different gene expressions under different pathological conditions have been shown to alter the SR composition and produce Ca2+-handling abnormalities in cardiomyocytes for the development of cardiac dysfunction. The post-translational modifications of SR Ca2+-cycling proteins by processes such as oxidation, nitrosylation, glycosylation, lipidation, acetylation, sumoylation, and O-GlcNacylation have also been reported to affect the SR Ca2+-release and uptake activities as well as cardiac contractile activity. The SR function in the heart is also influenced in association with changes in cardiac performance by several hormones including thyroid hormones and adiponectin as well as by exercise-training. On the basis of such observations, it is suggested that both Ca2+-cycling and regulatory proteins in the SR membranes are intimately involved in determining the status of cardiac function and are thus excellent targets for drug development for the treatment of heart disease.