The aim of the present study was to investigate the reflection of psychoemotional stress in the body surface potential distribution as documented by isointegral maps of cardiac activation and recovery. In 72 young men (18.3 ± 7.3 y.) with no cardiovascular history body surface potential maps (BSPMs) at rest and during the test of mental arithmetic were recorded. The digitalized data for each point of the QRS, STT and QRST integral maps, for each subject in both situations, were processed and evaluated by methods of univariate as well as spatial mathematical and statistical modeling. The results showed during MA a significant decrease of repolarization integral values over the sternum and
right precordium, which contributed to analogically localized decrements
also in the QRST BSM. The decrease occurred in more than 2/3 of lead points. The most pronounced changes were observed in the right precordial area, where potentials decreased in more than in 70 % of subjects. In conclusion, the discriminative power of the difference STT and QRST integral maps was strong enough to distinguish the mental arithmetic induced changes in the superficial cardiac electric field. These adrenergic transient alterations in ventricular recovery may be of importance in subjects at risk for ventricular arrhythmias.
Protective effect of quercetin, a natural antioxidant compound, on hypericin-induced cytotoxicity was studied in human promyelocytic leukemia cells (HL-60). Hypericin (10-5 mol.l-1) alone significantly decreased cell survival to 21 % that found in the controls, whereas in combination with quercetin (10-5 mol.l-1) this decrease was diminished to 46 %. Lower concentrations of quercetin had no protective effect. These findings indicate that oxygen radicals can play an important role in hypericin-induced phototoxic effects., A. Miroššay, H. Onderková, L. Mirossay, M. Šarišský, J. Mojžiš., and Obsahuje bibliografii
Non-alcoholic fatty liver disease (NAFLD) is an important cause of liver-related morbidity and mortality. The aim of this work was to establish and characterize a nutr itional model of NAFLD in rats. Wistar or Sprague-Dawley male rats were fed ad libitum a standard diet (ST-1, 10 % kcal fat), a medium-fat gelled diet (MFGD, 35 % kcal fat) and a high-fat gelled diet (HFGD, 71 % kcal fat) for 3 or 6 weeks. We examined the serum biochemistry, the hepatic malondialdehyde, reduced glut athione (GSH) and cytokine concentration, the respiration of liver mitochondria, the expression of uncoupling protein-2 (UCP-2) mRNA in the liver and histopathological samples. Feeding with MFGD and HFGD in Wistar rats or HFGD in Sprague-Dawley rats induced small-droplet or mixed steatosis without focal infl ammation or necrosis. Compared to the standard diet, there were no significant differences in serum biochemical parameters, except lower concentrations of triacylglycerols in HFGD and MFGD groups. Liver GSH was decreased in rats fed HFGD for 3 weeks in comparison with ST-1. Higher hepatic malondialdehyde was found in both strains of rats fed HFGD for 6 weeks and in Sprague-Dawley groups using MFGD or HFGD for 3 weeks vs. the standard diet. Expression of UCP-2 mRNA was increased in Wistar rats fed MFGD and HFGD for 6 weeks and in Sprague-Dawley rats using HFGD for 6 weeks compared to ST-1. The present stud y showed that male Wistar and Sprague-Dawley rats fed by HFGD developed comparable simple steatosis without signs of progression to non-alcoholic steatohepatitis under our experimental conditions., O. Kučera ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
b1_The purpose of this study was to assess the influence of aerobic training on the left ventricular(LV) systolic function. Thirty patients with stable coronary artery disease, who had participated in the conducted 3-month physical training, were retrospectively divided into 2 co horts. While patients in the cohort I(n=14) had continued training individually for 12 months, patients in the cohort II(n=16) had stopped training after finishing the conducted program. Rest and stress dobutamine/atropine echocardiography was performed in all patients before the training program and 1 year later. The peak systolic velocities of mitral annulus (Sa) were assessed by tissue Doppler imaging for individual LV walls. In addition, to determine global LV systolic longitudinal function, the four-site mean systolic velocity was calculated (Sa glob). According to the blood supply, left ventricular walls were divided into 5 groups: A-walls supplied by nonstenotic artery; B-walls supplied by coronary artery with stenosis ≤ 50 %; C-walls supplied by coronary artery with stenosis 51-70 %; D-walls with stenosis of supplying artery 71-99 %; and E-walls with totall y occluded supplying artery. In global systolic function, the follow-up values of Sa glob in cohort I were improved by 0.23±0.36 as compared with baseline values at rest, and by 1.26±0.65 cm/s at the maximal load, while the values of Sa glob in cohort II were diminished by 0.53±0.22(p=NS), and by 1.25±0.45 cm/s(p<0.05), respectively. Concerning the resting regional function, the only significant difference between cohorts in follow-up changes was found in walls E:0.37±0.60 versus -1.76± 0.40 cm/s(p<0.05). At the maximal load, the significant difference was found only in walls A(0.16±0.84 versus -2.67±0.87 cm/s; p<0.05)., b2_Patients with regular 12-month physical activity improved their global left ventricle systolic function mainly due to improvement of contractility in walls supplied by a totally occluded coronary artery., R. Panovský ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
We tested risperidone and ritanserin, serotonin-S2 receptor antagonists, for their effects on in vitro polyclonal IgG and IgM synthesis by human peripheral blood mononuclear cells (PBMC) stimulated with pokeweed mitogen (PWM). On the basis of the previously reported effect on immune function in vivo risperidone in this study was tested in three different groups of PBMC: healthy donors as well as schizophrenic patients before risperidone treatment and schizophrenic patients after the treatment with risperidone. IgG and IgM production after 7 days of culture was measured by ELISA. Risperidone decreased IgG synthesis (p<0.05) in PBMC of healthy subjects only at the highest concentration (10~6 M) and IgG synthesis enhanced by 5-HT was antagonized by risperidone. This effect, however, was not statistically significant. Neither risperidone nor ritanserin, in the concentration range 10-8- 10~6M, affected IgM synthesis in this group. Risperidone did not affect the production of IgG and IgM by PBMC of schizophrenic subjects in PWM-stimulated cultures both before and after risperidone therapy. The spontaneous production of IgG in PBMC of schizophrenic subjects before therapy was decreased (p<0.05) at concentrations 10-6-10~7 M of risperidone. We conclude that risperidone and ritanserin did not increase polyclonal IgG and IgM synthesis in vitro in contrast to neuroleptics currently used in clinical practice.
Diabetes mellitus is associated with a number of prothrombotic abnormalities, and correction of these abnormalities might translate into the reduction of cardiovascular risk. Glitazones improve endothelial function and reduce inflammation, but much less is known about their effect on thrombogenic factors. We have therefore studied the effect of rosiglitazone on leukocyte and soluble thrombogenic markers in patients with type 2 diabetes mellitus. Thirty-three subjects with type 2 diabetes and 32 normal controls were included; patients were examined at baseline and after 5 months of rosiglitazone treatment (4 mg/day). We measured leukocyte-platelet aggregates and leukocyte expression of either P-selectin glycoprotein ligand 1 (PSGL-1) or receptor for urokinase-type plasminogen activator (uPAR) using flow cytometry, as well as several circulating soluble thrombogenic markers by ELISA method. Leukocyte expression of uPAR and PSGL-1 was significantly higher in patients than in controls. Leukocyte-platelet aggregates and leukocyte expression of uPAR and PSGL-1 significantly decreased after rosiglitazone. There was also significant decrease in CRP and fibrinogen levels, but there was no effect of diabetes and/or rosiglitazone on other circulating molecules. In conclusions, we observed a substantial improvement in the expression of thrombogenic markers on leukocytes after rosiglitazone treatment, suggesting the novel antithrombotic effects of rosiglitazone., H. Svobodová ... [et al.]., and Obsahuje seznam literatury
We established a multifactoral long-term field experiment at the Látókép experimental site of the University of Debrecen (Debrecen, Hungary), on mid-heavy calcareous chernozem soil in 1984, using experimental data from 17 years (1990-2008). We examined the extent to which soil fertility affects maize yield under natural conditions (without fertilisation). We analysed the effect of precipitation in the winter period (from the harvest of the previous crop (maize) until sowing (i.e. October-March)) and the growing season (i.e. April-September) on yield and we evaluated yield per FAO group. We examined the joint effect of crop year and hybrid maturity groups on maize yield; then we evaluated how hybrid maturity groups per crop year and wet and dry years per ripening group affected maize yield. It was shown that the pH value of soil significantly affected yield and also that there was a strong positive correlation between pH value and yield (r = 0.81) at a 1% significance level. The correlation between the two variables is described by a linear regression line. The slope shows that a 0.1 soil pH increase results in a 510 kg ha-1 maize yield increase on average. The correlation between the amount of precipitation during the growing season and maize yield is average, positive (r = 0.718) and linear. Based on the parameters of the estimated regression line - within non-fertilised conditions - 1 mm increase of precipitation resulted in a 9 kg ha-1 increase in yield. The analysis of the joint effect of hybrid maturity groups and crop year on yield showed that crop year determines standard deviation six times more than hybrid maturity groups, whereas the effect their interaction was not significant. and Práca bsahuje výsledky multifaktorového, dlhodobého pokusu na experimentálnej ploche Látókép, Univerzity v Debrecíne (Debrecen, Maďarsko). Experiment prebiehal od roku 1984, v tejto práci boli použité údaje z experimentu získané počas 17tich rokov (1990-2008). Pôda je stredne ťažká černozem. Bol skúmaný vplyv pôdnej úrodnosti na úrodu kukurice v prirodzených podmienkach, bez hnojenia. Študoval sa predovšetkým vplyv zrážok počas zimného obdobia (od zberu úrody kukurice až po nasledujúcu sejbu, t.j. od októbra do marca) a vplyv zrážok počas vegetačného obdobia (apríl -september) na úrodu kukurice. Okrem toho sa študoval vplyv ''sezóny'' a skupín skorosti hybridov na úrodu kukurice. Bolo vyhodnotený tiež vplyv skupiny skorosti hybridov počas suchých a mokrých rokov na úrodu kukurice. Bolo preukázané, že hodnota pH pôdy významne ovplyvňuje úrodu; súčiniteľ lineárnej korelácie medzi pH a úrodou na úrovni významnosti 1% bol r = 0,81. Z tejto korelácie vyplýva, že zvýšenie pH o 0,1 spôsobuje zvýšenie úrody kukurice priemerne o 510 kg ha-1. Korelácia medzi úrnom zrážok počas vegetačného obdobia kukurice a úrodou je pozitívna a lineárna (r = 0,718), z analýzy vyplýva, že zvýšenie úhrnu zrážok o 1 mm spôsobí priemerné zvýšenie úrody kukurice o 9 kg ha-1. Z výsledkov analýzy spoločného vplyvu skupín skorosti hybridov a sezóny vyplýva, že konkrétna sezóna ovplyvňuje tvorbu úrod 6-krát významnejšie, ako skupina skorosti hybridov; interakcia medzi skupinami skorosti hybridov a vlastnosťami sezóny nie je významná.
Soil water repellency causes at least temporal changes in the hydrological properties of a soil which result in, among other things, suboptimal growing conditions and increased irrigation requirements. Water repellency in soil is more widespread than previously thought and has been identified in many soil types under a wide array of climatic conditions worldwide. Consequences of soil water repellency include loss of wettability, increased runoff and preferential flow, reduced access to water for plants, reduced irrigation efficiency, increased requirement for water and other inputs, and increased potential for non-point source pollution. Research indicates that certain soil surfactants can be used to manage soil water repellency by modifying the flow dynamics of water and restoring soil wettability. This results in improved hydrological behavior of those soils. Consequently, the plant growth environment is also improved and significant water conservation is possible through more efficient functioning of the soil. and Vodoodpudivosť pôdy spôsobuje prinajmenšom dočasné zmeny v hydrologických vlastnostiach pôdy, ktoré okrem iného môžu viesť k suboptimálnym podmienkam rastu rastlín a k zvýšenej potrebe závlah. Vodoodpudivosť pôdy je rozšírenejší jav, ako sa pôvodne predpokladalo; bola identifikovaná v mnohých pôdnych typoch a klimatických podmienkach na celom svete. Dôsledkom vodoodpudivosti pôdy je strata zmáčavosti, zvýšený povrchový odtok a preferenčné prúdenie, znížená dostupnosť vody a iných vstupov pre rastliny, znížený účinok závlah, zvýšené požiadavky na vodu a iné vstupy, ako aj zvýšené riziko plošného znečistenia. Výskum naznačuje, že niektoré povrchovo aktívne látky (soil surfactants) môžu upraviť vodoodpudivosť pôdy obnovením omáčania a modifikáciou dynamiky vody. Výsledkom je zlepšenie hydrologických vlastností pôdy. Podobne, výsledkom je zlepšenie prostredia pre rast rastlín, zvýšenie retencie vody v pôde a teda aj efektívnejšia funkcia pôdy.