Tomato samples were collected from the field of Absheron peninsula in Azerbaijan in order to evaluate the incidence of main Tobamoviruses. According to results of serological and molecular tests, Tomato mosaic virus (ToMV), Tobacco mosaic virus (TMV), and Pepper mild mottle virus (PMMoV) were detected as single and mixed infections (TMV + PMMoV; ToMV + PMMoV) in various tomato samples. It was found that Tobamovirus infection caused an increase in the content of malondialdehyde, alterations in the activities of peroxidase enzymes and quantitative and qualitative changes in their molecular isoforms. A comparison of thylakoid membrane polypeptides from virus-infected leaves indicated a decrease in the content of the thylakoid membrane polypeptides with molecular masses of 123, 55, 47, 33, 28-24, 17, and 15 kD. PSII efficiency and the content of chlorophylls (a and b) were significantly lower in the virus-infected leaves., I. M. Huseynova, S. M. Mirzayeva, N. F. Sultanova, D. R. Aliyeva, N. Sh. Mustafayev, J. A. Aliyev., and Obsahuje bibliografii
Virotherapy: The viruses that can cure. The viruses that can selectively replicate in and destroy neoplastic cells are called oncolytic. The idea of using these viruses as anticancer drugs goes back to the beginning of the 20th century. Since than various oncolytic viruses have been taken to clinical trials. Oncolytic viruses can be classified largely into two groups: naturally oncolytic viruses (mainly RNA viruses) and genetically engineered viruses to achieve councer specificity (usually DNA viruses). The review is focused on the basic scientific principles used for development of oncolytic viruses and summarize current results from clinical trials. The eligibility, feasibility and safety of oncolytic virus therapy as a novel therapeutic agent against cancer is discussed.
Mechanical behavior of biological structures under dynamic loading generally depends on elastic as well as viscous properties of biological materials. The significance of “viscous” parameters in real situations remains to be elucidated. Behavior of rheological models consisting of a combination of inertial body and two Voigt’s bodies were described mathematically with respect to inverse problem solution, and behavior in impulse and harmonic loadings was analyzed. Samples of walls of porcine and human aorta thoracica in transverse direction and samples of human bone (caput femoris, substantia compacta) were measured. Deformation responses of human skin in vivo were also measured. Values of elastic moduli of porcine aorta walls were in the interval from 102 kPa to 103 kPa, values of viscous coefficients were in the interval from 102 Pa.s to 103 Pa.s. The value of shear stress moduli of human caput femoris, substantia compacta range from 52.7 to 161.1 MPa, and viscous coefficients were in the interval from 27.3 to 98.9 kPa.s. The role of viscous coefficients is significant for relatively high loading frequencies - in our materials above 8 Hz in aorta walls and 5 Hz for bones. In bones, the viscosity reduced maximum deformation corresponding to short rectangular stress., M. Kuchařová, S. Ďoubal, P. Klemera, P. Rejchrt, M. Navrátil., and Obsahuje bibliografii
Dependence of ATP hydrolysis kinetics by the chloroplast coupling factor (CF1) on medium viscosity was studied at varying temperatures. For samples with oxidized and reduced CF1 γ-subunit, this dependence was shown to be described by Cramers’ relationship k - (η/ηo)-n, where k is the reaction rate constant, η/ηo is the medium/water viscosity ratio, and 0 < n < 1. Transition of the γ-subunit from its reduced to oxidized state was accompanied by increasing n value, which is indicative of increasing friction losses between certain enzyme sections and the solution. The increased medium viscosity produced no effect on the reaction activation energy which appeared to be almost the same for the both enzyme states. The molecular mechanisms responsible for CF1 activity loss in viscous media are discussed., A.N. Malyan., and Obsahuje bibliografii