Progeny of the flesh fly Sarcophaga bullata exposed to short day length show a maternal effect that prevents the expression of pupal diapause. Although ecological aspects of this effect are well studied, not enough is known about the molecular mechanisms underlying this maternal effect. In this study, two-dimensional electrophoresis was performed to detect differences of the abundance of certain proteins in the ovaries of this fly kept under long day and short day conditions for 2 days after eclosion. Eleven proteins that were abundant and showed significant changes were selected for mass spectrometric identification. Ovary proteins that increased in abundance under short-day conditions were similar to twinstar CG4254-PA, muscle protein 20-like protein, GA13413-PA, gene analogous to small peritrophins (Gasp CG10287-PA) and Ribosomal protein LP1 CG4087-PA. Ovary proteins that decreased in abundance under short-day conditions were similar to the ATP synthase beta subunit, fk506-binding protein and storage protein-binding protein. The 2-D proteome maps included 2 additional unknown proteins that were more abundant and 1 that was less abundant in the ovaries of 2-day old short-day females. Twinstar CG4254-PA, muscle protein 20-like protein and GA13413-PA harbour an actin-binding domain. That the 3 actin-binding proteins increase in abundance suggests that it is likely that an alteration in the actin cytoskeleton is involved in this maternal effect in the flesh fly., Aiqing Li ... [et al.]., and Obsahuje seznam literatury
The importance of stream corridors and flood deposits for the spreading of molluscs is demonstrated using some examples from the Czech territory. Three types of floodplain debris, which contain shell accumulation, are distinguished: allochtonous debris accumulated by river flow on riversides, autochtonous accumulation in quiet parts of alluvium far from the main flow, and accumulation caused by strong turbulence during flooding. and Vojen Ložek, Lucie Juřičková.
Varanovec bornejský (Lanthanotus borneensis) patří k nejvzácnějším a nejméně známým plazům. V článku je popsána historie jeho objevů a její průběh ve 20. století, dále jeho anatomie, osteologie, morfologie a fylogeneze. Jsou podány i vzácné informace o jeho ekologii. Je v něm popsán i první nález tohoto druhu ve Východním Kalimantanu (Borneo, Indonésie) a první poznatky z chovu a chování varanovce v podmínkách terária v japonské zoo. Kameraman Stano Slušný a etnolog Miroslav Nožina viděli v roce 2001 uloveného živého varanovce ve vsi Respen na břehu řeky Sesayap v okrese Malinau na severu indonéské provincie Východní Kalimantan. Z literatury vyplývá, že šlo o první pozorování varanovce bornejského nejen v této provincii, ale v té době dokonce o první nález tohoto druhu na území Indonésie., The Earless Monitor Lizard (Lanthanotus borneensis) is one of the rarest and least known of all reptiles. This article describes the history of discoveries throughout the 20th century, as well as its anatomy, osteology, morphology and phylogenesis. Valuable information on its ecology is also provided. The first discovery of this species in Eastern Kalimantan (Borneo, Indonesia) – Respen by Sesayap river in 2001 – by Czech travellers and the first findings from breeding and rearing of these lizards in terrarium conditions at a zoo in Japan are also reported., and Ivan Vergner.
Procesům adaptace na život v lidských sídlech podléhají mnohé druhy ptáků nejenom v Evropě, ale i v Latinské Americe. Během několika cest po tropických zemích Nového světa od Mexika po Argentinu autoři zaznamenali v tamních městech i na venkově několik desítek ptačích druhů se sklonem k synantropnímu způsobu života. V sumáru 57 druhů z patnácti čeledí, které byly zařazeny do článku, dominují pěvci (41 druhů). Přestože jde o subjektivní výběr a jednotlivé druhy ptáků nebylo možné podrobněji charakterizovat, uvedené informace mohou návštěvníkům této oblasti usnadnit orientaci při setkání se zástupci avifauny latinskoamerických sídel., Many bird species are involved in processes of adaptation to life in human settlements, not only in Europe, but also in Latin America. During several trips to tropical countries in the New World, from Mexico to Argentina, the authors noted several dozen bird species in the cities and the countryside that tended to adopt a synanthropic lifestyle. Songbirds predominate (with 41 species) in a list of 57 species from the 15 families presented. The article may help visitors to the region find their bearings among the birdlife in Latin American conurbations., and Jozef Májský, Stanislav Chudý.
A half-century of zoological investigations in the region of Podblanicko (Central Bohemia) published in the regional collection Nature and History of Podblanicko Region.
Quametopia gen. n. is established on the basis of recent phylogenetic analysis of taxa formerly assembled under the genus Mumetopia Melander, 1913 to include M. terminalis (Loew, 1863) and two new closely related Nearctic species of Anthomyzidae. The new genus is diagnosed and its phylogenetic relationships discussed. Quametopia terminalis (Loew, 1863) comb. n. is transferred from Mumetopia and redescribed based on revision of the type material (lectotypes of Anthophilina terminalis Loew, 1863 and its synonym Mumetopia nitens Melander, 1913 are designated) and other extensive material. Quametopia clintonia sp. n. and Q. amplistylus sp. n. (both from Canada, USA) are described and relationships of all three Quametopia species discussed. Immature stages of Q. terminalis and Q. clintonia sp. n. obtained by means of adult-to-adult rearing are described (1st- and 2nd-instar larvae for the first time in the family Anthomyzidae) and illustrated. Keys to adults, eggs, larvae and puparia of Quametopia species are presented. Biology (habitat and host-plant associations, life history) of Q. terminalis and Q. clintonia sp. n. are studied in detail and their ecological separation demonstrated. Distribution of all Quametopia species is reviewed. and Jindřich Roháček, Kevin N. Barber.
Pyridoxal kinase (PLK; EC 2.7.1.35) is a key enzyme in the metabolism of vitamin B6 (VB6) in Bombyx mori. A fusion expressional vector pET-22b-BPLK-His was constructed using a sub-cloning technique, the recombinant B. mori PLK was then expressed in Escherichia coli, purified and characterized. Bioinformatics were used to deduce the protein structure and genomic organization of this enzyme. Using Ni Sepharose affinity column chromatography, the recombinant protein was purified to very high degree (approximately 90%). The recombinant PLK exhibits a high specific enzymatic activity (1800 nmol/min/mg of protein). The maximum catalytic activity of this enzyme was recorded over a narrow pH range (5.5-6.0) and Zn2+ is the most effective cation for catalysis under saturating substrate concentrations. When only triethanolamine is present as the cation, K+ is an activator of PLK. A double reciprocal plot of initial velocity suggests that the enzyme catalyses the reaction by means of a sequential catalytic mechanism. Under optimal conditions, the Km value for the substrates of ATP and pyridoxal are 57.9 ± 5.1 and 44.1 ± 3.9 µM. B. mori's genome contains a single copy of the PLK gene, which is 7.73 kb long and contains five exons and four introns, and is located on the eighth chromosome. The PLK may be a dimer with two identical subunits under native conditions, and it is hypothesized that each monomer contains eight α-helices (α1-8), nine β-strands (β1-9) and two segments of 310 helices. and Shuo-Hao HUANG, Wang MA, Ping-Ping ZHANG, Jian-Yun ZHANG, Yan-Feng XIE, Long-Quan HUANG.