Larvae of Dendrolimus punctatus overwinter in diapause. In the Yangtze River Region, this species is multivoltine at altitudes below 400 m and univoltine above 700 m a.s.l. The photoperiodic response of the univoltine population, investigated at five day lengths (11.5, 12.5, 13.5, 14.5 and 15.5 h) at 27 + 1C, indicates that D. punctatus is a long-day species. Diapause was induced in 95-100% of the individuals by three short photophases (11.5-13.5 h), while diapause incidence decreased steeply with increase in day length down to 10% at 15.5 h. The critical daylength (CDL = day length resulting in a 50% incidence of diapause) is 14.6 h. When induced by critical or longer photophases (14.5 and 15.5 h), diapause terminated spontaneously, without any change in photoperiod or temperature. Diapause induced at shorter photophases was more intense and not terminated without activation by diapause averting conditions. Larvae in diapause lived for up to 190 d when kept under a 11.5 h photophase during which they remained sensitive to the photoperiodic signal. In transfer experiments, diapause was terminated after an increase in photoperiod, even if it was within the range of diapause inducing photoperiods: from 11.5 h to 13.5 h. This study revealed that the univoltine hill dwelling population of D. punctatus undergoes a facultative diapause. Although potentially multivoltine they are univoltine because of the low temperatures at that altitude., Ju-Ping Zeng, Yong Wang, Xing-Ping Liu., and Obsahuje seznam literatury
1_The breakdown of the transhumant grazing system in the Spanish Pyrenees has led to a severe decline in the area of pastures. However, in the high mountain zone there are still large areas of species-rich grasslands. The aim of this study was to assess the oviposition preferences of the shrub-feeding Blue-spot hairstreak, Satyrium spini (Denis & Schiffermüller, 1775), in montane common pastures in the Spanish Pyrenees and recommend a way of managing these grasslands that favours this species. Our study showed that females of S.spini laid their eggs on Dwarf buckthorn (Rhamnus pumila Turra) and Alpine buckthorn (R. alpina L.), which are novel host plant records for Spain. A warm microclimate was of crucial importance for egg-laying. Occupied plants grew mostly at sparsely vegetated grassland sites where there were large patches of bare rocks, stones or gravel. Most egg batches were laid close to the ground and 75% consisted of only one egg. The number of batches per R. pumila plant was higher on east-, south- and west-facing slopes than on north-facing slopes. Presence of eggs and the number of egg batches per R. pumila plant were best explained by a long sunshine duration. At high altitudes particularly warm microhabitats seem to be more important for S.spini than at lower altitudes in Central Europe. We assume that the preference for unusually warm microhabitats is explained by the cold climatic conditions near the altitudinal range limit of the species. That most of the eggs were laid singly and not in small batches as in Central Europe might be a risk-spreading strategy to cope with the harsh climatic conditions and the high inter-annual variation in weather conditions in the high mountain zone in the Pyrenees., 2_The best way to maintain open grasslands for S. spini and other thermophilous grassland species in the high mountain zone of the Pyrenees is to use the traditional combination of sheep and cattle grazing., Gregor Stuhldreher, Luis Villar, Thomas Farthmann., and Obsahuje seznam literatury
Insect cold tolerance mechanisms are often divided into freezing tolerance and freeze intolerance. This division has been criticised in recent years; Bale (1996) established five categories of cold tolerance. In Bale's view, freezing tolerance is at the extreme end of the spectrum of cold tolerance, and represents insects which are most able to survive low temperatures. Data in the literature from 53 species of freezing tolerant insects suggest that the freezing tolerance strategies of these species are divisible into four groups according to supercooling point (SCP) and lower lethal temperature (LLT): (1) Partially Freezing Tolerant-species that survive a small proportion of their body water converted into ice, (2) Moderately Freezing Tolerant-species die less than ten degrees below their SCP, (3) Strongly Freezing Tolerant-insects with LLTs 20 degrees or more below their SCP, and (4) Freezing Tolerant Species with Low Supercooling Points which freeze at very low temperatures, and can survive a few degrees below their SCP. The last 3 groups can survive the conversion of body water into ice to an equilibrium at sub-lethal environmental temperatures. Statistical analyses of these groups are presented in this paper. However, the data set is small and biased, and there are many other aspects of freezing tolerance, for example proportion of body water frozen, and site of ice nucleation, so these categories may have to be revised in the future. It is concluded that freezing tolerance is not part of Bale's (1996) continuum, but rather a parallel, alternative strategy of cold tolerance., Brent J. Sinclair, and Lit
The intertidal collembolan Anurida maritima can endure periods of twice-daily submergence by seawater. The air-breathing terrestrial apterygote insect has developed specific adaptations to prevent respiratory failure during hypoxic stress. When submerged, the animal is initially enclosed by an air-bubble. This bubble lasts three times longer than the small amount of stored oxygen would allow. The air bubble acts not only as an oxygen store but also as a compressible gas gill. This was demonstrated by an O2-needle electrode technique which allowed recordings of pO2 changes in the watery medium close to the animal. Oxygen uptake in A. maritima follows an aerial mode of respiration during the first three hours of submergence. Oxygen consumption rate at decreasing ambient O2 partial pressures showed oxyregulating behaviour during severe hypoxia., Dietmar Zinkler, Raimund Rüssbeck, Marc Biefang, Horst Baumgärtl, and Lit
Larval Manduca prothoracic gland cells in vitro responded to prothoracicotropic hormone (PTTH) from neurosecretory cells of the brain with an increase of intracellular free calcium. This effect is reversible and dose-dependent. Preincubation of the glands with TMB-8 and dantrolene, which inhibit the release of calcium from intracellular stores, did not decrease the PTTH-stimulated increase in calcium, indicating that intracellular calcium stores are not involved in the control of ecdysteroidogenesis. Pharmacological studies of the PTTH effect with calcium channel blockers revealed that the increase in calcium was totally blocked by cadmium, partially inhibited by nickel and lanthanum and by amiloride, an antagonist of T-type calcium channels. All other inhibitors tested were ineffective, suggesting that the increase in cytosolic calcium is induced by opening of calcium channels, presumably of the T-type, in response to PTTH. The action of PTTH on these channels may be mediated by a G-protein as shown by the effect of mastoparan, a G-protein activator, which increased the concentration of cytosolic calcium comparable to that evoked by PTTH., Heiner Birkenbeil, and Lit
As urbanisation is set to continue, understanding the impact on wildlife becomes increasingly important if we are to be able to conserve biodiversity. As an excellent group of bioindicators, invertebrates can allow us to understand some of the forces in urban areas which impact upon biodiversity and wildlife populations. This paper discusses some of the trends in the abundance, diversity and richness of invertebrates related to urbanisation and the specific urban environmental and traffic factors which may be at play., Elizabeth L. Jones, Simon R. Leather., and Obsahuje seznam literatury
Coloured Moericke water pan traps were used to determine the effect of colour on the preference behaviour of butterflies (Lepidoptera: Hesperioidea, Papilionoidea) over the period 2001 to 2003 in grassland habitats in Eastern Slovakia (Košická kotlina basin). A total of 912 individuals belonging to 53 species and 7 families of butterflies were trapped. The colour of the traps that caught the most butterflies was white, followed by blue, violet, yellow and finally the least were caught by red coloured traps. Ordination analysis showed that some butterfly families and species were more likely to be caught by traps of a specific colour. Measurements of the wavelengths of the colours used revealed that butterflies preferred short-wavelengths light. The effect of colour on the catches did not differ significantly among the sites. The butterflies were more likely to be caught by traps of a certain colour even though the other features of the traps were the same., Lenka Kočíková ... [et al.]., and Obsahuje seznam literatury
The carob moth, Ectomyelois ceratoniae (Lepidoptera: Pyralidae), is a serious pest and causes a considerable loss of yield of pomegranate in Iran. Apanteles myeloenta (Hymenoptera: Braconidae) is the dominant parasitoid of this pest parasitizing it more than 30% in recent years. This parasitoid is a candidate for augmentative biological control program to reduce the level of the infestation of fruit overwinter. The objective of this research was to optimize the mass production of A. myeloenta. The mean developmental time of females was 28 days and of males 27 days. Pupal development lasted 7 days. The second instar was the preferred host and most heavily parasitized (45%). Wasps that started their development in second instar hosts produced the highest progeny and those that started in third instar hosts survived as adults for longest. The sex ratio (females to males) of A. myeloenta that emerged from carob moth larvae parasitized in the first instar was 1 : 3.5, in the second instar 1 : 3 and in the third instar 1 : 2. The influence of different host ages on the functional response of A. myeloenta to host density was assessed. Logistic regression indicated a type II functional response to different densities of all the stages of the host tested., Hossein Kishani Farahani, Seyed Hossein Goldansaz., and Obsahuje seznam literatury
Béthoux recently identified the species †Adiphlebia lacoana Scudder from the Carboniferous of Mazon Creek, Ill., USA as the oldest beetle. The fossils bear coriaceous tegmina with pseudo-veins allegedly aligned with "rows of cells" as they occur in Permian beetles and extant Archostemata. The examination of four new specimens of †Adiphlebia lacoana from the same locality revealed that the "cells" are in fact clumps of clay inside a delicate meshwork, and no derived features shared with Coleoptera or Coleopterida (= Coleoptera + Strepsiptera) were found. Instead, †Adiphlebia lacoana bears veinal fusions and braces similar to extant Neuroptera. These features support a placement in †Strephocladidae, and are also similar to conditions found in †Tococladidae. These unplaced basal holometabolan families were erroneously re-analyzed as ancestral Mantodea and Orthoptera. Homologization of the wing pairs in neopteran lineages is updated and identification errors are corrected. A new Permian beetle family †Moravocoleidae [†Protocoleoptera (= Permian Coleoptera with pointed unpaired ovipositor; e.g., †Tshekardocoleidae)] is described., Jarmila Kukalová-Peck, Rolf G. Beutel., and Obsahuje seznam literatury
This paper describes the characterization of a set of nine microsatellite markers for the pipe-organ mud-dauber wasp, Trypoxylon albitarse F. (Hymenoptera: Crabronidae), a common solitary wasp species in Brazil. Eight of the nine loci described were found to be polymorphic. The number of alleles ranged from three to 11. Observed and expected heterozygosity on the population level ranged from 0.135 to 0.891 and 0.177 to 0.865, respectively. Genotypic analyses revealed no deviations from Hardy-Weinberg equilibrium or linkage disequilibrium after sequential Bonferroni correction. Cross-species amplification was also tested for nine related species and positive amplifications were found in most of the assays. These markers will be useful in studies involving relatedness analysis and investigations of the population genetics of these wasps., Juliano Da Costa Almeida ... [et al.]., and Obsahuje seznam literatury