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2. First record of metacestodes of Mesocestoides sp. in the common starling (Sturnus vulgaris) in Europe, with an 18S rDNA characterisation of the isolate
- Creator:
- Literák, Ivan, Olson, Peter D., Georgiev, Boyko B., and Špakulová, Marta
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- Mesocestoides, Sturnus vulgaris, metacestodes, tetrathyridia, Cestoda, and birds
- Language:
- English
- Description:
- Metacestodes of Mesocestoides sp. were recorded from Sturnus vulgaris (Passeriformes: Sturnidae) in the Czech Republic in April 2002. They were found in a cutaneous cyst and in the thoracic region of the body cavity of the bird. This is the first record of metacestodes of Mesocestoides sp. in this host species in Europe as well as the first finding of the formation of a cutaneous cyst provoked by this parasite. Additional specimens from Apodemus agrarius (Mammalia: Rodentia) from Bulgaria and Lacerta agilis (Reptilia: Squamata) from the Czech Republic were compared with that from S. vulgaris. Sequence data from the V4 variable region (18S rDNA) were used to compare genetic variability among these and previously characterized isolates of Mesocestoides spp. A number of distinct clades were recognized, with metacestodes from L. agilis showing the highest degree of relative divergence.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3. Morphology and taxonomy of Stomylotrema (Digenea: Stomylotrematidae) representatives from ciconiiform and podicipediform birds in Cuba
- Creator:
- Macko, Josef Kazimír, Špakulová, Marta, and Casanova, Juan Carlos
- Format:
- Type:
- model:internalpart and TEXT
- Subject:
- digenetic trematodes, Stomylotrema bijugum, Stomylotrema vicarium, Cuba, variability, taxonomy, Ajaia ajaja, Egretta caerulea, and Podiceps dominicus dominicus
- Language:
- English
- Description:
- Two Stomylotrema Looss, 1900 species were found in storks and grebes in Cuba. Five specimens of Stomylotrema bijugum Braun, 1901 were recovered from the roseate spoonbill, Ajaia ajaja (Allen, 1942) (Ciconiiformes) while six Stomylotrema vicarium Braun, 1901 were found in the little blue heron, Egretta (syn. Florida) caerulea (L.) (Ciconiiformes) and the least grebe Podiceps dominicus dominicus (I,.) (Podicipediformes). The taxa represent new host and geographical records. The problem of morphological variation within the genus Stomylotrema is briefly analysed in the discussion.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
4. Tapeworm chromosomes: their value in systematics with instructions for cytogenetic study
- Creator:
- Orosová, Martina and Špakulová, Marta
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- tasemnice, cytogenetika, tapeworms, cytogenetics, karyotype, methodological manual, 2, and 59
- Language:
- English
- Description:
- The history and value of cytogenetic features for addressing questions of the evolution and systematics of tapeworms (Cestoda) are briefly reviewed along with instructions for collecting karyological data. As a supplement to worm morphology, chromosome number and morphology have been helpful in determining the systematic status of some genera in the Diphyllobothriidae and species in the Bothriocephallidea. In addition, many new techniques for chromosome analysis have been recently applied in morphological and molecular studies of invertebrates, including tapeworms. Methods of molecular karyology, fluorescence in situ hybridisation, and chromosomal location of satellite DNA, microsatellites or histone genes may also provide useful data to inference of taxonomic relationships and for revealing trends or general lines of chromosome evolution. However, as karyological data are available only for few tapeworms, they are seldom an integral part of evolutionary and taxonomic studies of cestodes. A primary reason for this lack of karyological data may lie in general difficulties in working with tapeworm chromosomes. To address these problems, herein we present a well-tested, step-by-step illustrated guide on the fixation of tapeworm material and preparation of their chromosomes for cytogenetic studies. The technique requires standard glassware, few reagents and simple equipment such as needles; it can also be used on other neodermatan flatworms., Martina Orosová, Marta Špakulová., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public