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Start Over Creator Danihel, Ľudovít

2. Optimálne načasovanie laparoskopickej cholecystektómie v liečbe akútnej cholecystitídy

Creator:
Rajčok, Matúš, Danihel, Ľudovít, Bak, Vladimír, Oravský, Milan, and Schnorrer, Milan
Format:
print, text, and regular print
Type:
model:article, article, Text, and TEXT
Subject:
časové faktory, cholecystektomie laparoskopická--statistika a číselné údaje--využití, výsledky a postupy - zhodnocení (zdravotní péče), prospektivní studie, randomizované kontrolované studie jako téma, cholecystitida--chirurgie, ženské pohlaví, lidé, mužské pohlaví, and lidé středního věku
Language:
Czech, Slovak, and English
Description:
Úvod: Akútna cholecystitída je stále častým ochorením vyskytujúcim sa v rozvinutých krajinách sveta. Laparoskopická cholecystekómia (LCHE) je metódou liečby akútnej cholecystitídy, avšak pretrváva celosvetový nejednotný názor na načasovanie laparoskopickej cholecystektómie v liečbe akútnej cholecystitídy. Metoda: Od septembra 2012 do augusta 2015 sme realizovali na III. chirurgickej klinike UNsP Milosrdní bratia v Bratislave prospektívnu randomizovanú štúdiu. Porovnávali sme dva základné prístupy liečby akútnej cholecystitídy. Počas trvania štúdie sme hospitalizovali 64 pacientov s akútnou cholecystitídou, ktorí boli prijatí na chirurgické oddelenie. 32 pacientov bolo včasne operovaných do 72 hodín od objavenia príznakov ochorenia. Ďalších 32 pacientov bolo primárne liečených konzervatívne antibiotickou terapiou, a následne po 6−8 týždňoch sme vykonali odloženú cholecystektómiu. Výsledky: Z výsledkov nášho súboru vyplývajú viaceré výhody včasnej laparoskopickej cholecystektómie. Medzi výhody včasnej LCHE patria skrátenie operačného času, nižšia miera konverzií a zníženie pooperačnej záťaže. Ďalšou výhodou je skrátenie trvania hospitalizácie, a z toho vyplývajúce nižšie náklady na hospitalizáciu. Záver: Na základe popísaných výhod sa prikláňame k názoru, že tzv. immediate laparoscopic cholecystectomy (do 24 hodín od prijatia do nemocnice) by sa mala stať metódou voľby u pacientov s akútnou cholecystitídou. Kĺúčové slová: akútna cholecystitída – včasná a odložená laparoskopická cholecystektómia − prospektívna randomizovaná štúdia, Introduction: Acute cholecystitis is one of the most frequent diseases occurring in developed countries of the world. Laparoscopic cholecystectomy is a treatment option for acute cholecystitis. Since the advent of laparoscopic cholecystectomy there has been a lack of agreement regarding the timing of the operation in the treatment of acute cholecystitis. Method: From September 2012 to August 2015 we carried out a prospective randomized trial at the IIIrd Surgical Department of University Hospital Milosrdní bratia in Bratislava. We compared two basic approaches to the treatment of acute cholecystitis. During the trial, 64 patients with acute cholecystitis were admitted to the surgery department. 32 patients were treated with early laparoscopic cholecystectomy within 72 hours from the appearance of the symptoms. The other 32 patients were primarily treated with antibiotics and subsequently underwent delayed cholecystectomy after 6−8 weeks. Results: Our results suggest several advantages of early laparoscopic cholecystectomy such as shorter operation time, lower conversion rate, shorter length of hospital stay, shorter postoperative convalescence and lower cost of hospitalisation. Conclusion: Based on these results we believe that immediate laparoscopic cholecystectomy (within 24 hours from the patient’s admission to hospital) should become a preferred method of treatment of patients with acute cholecystitis., and M. Rajčok, Ľ. Danihel, V. Bak, M. Oravský, M. Schnorrer
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

4. PTEN mutations as predictive marker for the high-grade endometrial cancer development in Slovak women

Creator:
Gbelcová, Helena, Gergely, Lajos, Šišovský, Vladimír, Straka, Ľubomír, Böhmer, Daniel, Pastoráková, Andrea, Sušienková, Katarína, Repiská, Vanda, Korbeľ, Miroslav, Danihel, Ľudovít, and Priščáková, Petra
Format:
počítač and online zdroj
Type:
model:article and TEXT
Subject:
endometrial hyperplasia, endometrial carcinoma, PTEN, sequencing, and immunohistochemistry
Language:
English
Description:
Endometrial carcinoma (ECa) is one of the most common neoplasia of the female genital tract. The phosphatase and tensin (PTEN) homolog is the most frequently mutated tumor suppressor gene in endometrial carcinoma. PTEN encodes a phosphatase, a key regulatory enzyme involved in a signal transduction pathway that regulates cell growth, migration and apoptosis. The study evaluates an association between the morphological appearance of endometrial hyperplasia and ECa, and the presence of PTEN variations, PTEN protein level and intracellular localization. A total of 67 archived formalin-fixed and paraffin-embedded human biopsy tissue specimens with normal proliferative and secretory endometrium, endometrial hyperplasia without atypia and endometrial atypical hyperplasia, endometrioid the grade G1 and G3 and serous subtype of ECa were evaluated by sequencing for the presence of mutations in coding regions of PTEN gene of endometrial epithelial cells. The PTEN gene expression and intercellular localization of PTEN protein were evaluated immunohistochemically by immunoreactive score (IRS). PTEN mutation spectrum in endometrial carcinoma was identified for Slovak population. Twenty-eight non-silent mutations were identified in PTEN, twelve of them being novel, not annotated in Catalogue of Somatic Mutations in Cancer. Higher frequency of PTEN mutations was observed in serous carcinoma compared to global average. No correlation was observed between samples IRS, PTEN cellular localization and identified mutations. PTEN sequencing can be beneficial for patients considering prognosis of disease and sensitivity to treatment.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public