Search

Start Over Creator Jirků, Milan

1. A diagnostic assay based on variable intergenic region distinguishes between Leishmania donovani and Leishmania infantum

Creator:
Chocholová, Eva, Jirků, Milan, and Lukeš, Julius
Format:
bez média and svazek
Type:
model:article and TEXT
Language:
English
Description:
We have developed a PCR assay that in a single reaction distinguishes between Leishmania infantum and Leishmania donovani strains on the basis of different size of the amplicon. The targeted intergenic region between putative biopterin transporter and nucleotide binding protein on chromosome 35 is highly variable, species-specific and can be amplified from clinical samples. Based on the assay, five tested Leishmania archibaldi and L. infantum strains from the Sudan and Ethiopia clearly belong to L. donovani, which is in accordance with a recent multifactorial analysis of these strains. The nucleotide sequence reported in this paper has been submitted to the GenBankTM with accession number EU068004.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. How monoxenous trypanosomatids revealed hidden feeding habits of their tsetse fly hosts

Creator:
Votýpka, Jan, Petrželková, Klára J., Brzoňová, Jana, Jirků, Milan, Modrý, David, and Lukeš, Julius
Format:
počítač and online zdroj
Type:
model:article and TEXT
Subject:
Glossina, blood-feeding, adenotrophic viviparity, bodonids, Trypanosoma, and infection
Language:
English
Description:
Tsetse flies are well-known vectors of trypanosomes pathogenic for humans and livestock. For these strictly blood-feeding viviparous flies, the host blood should be the only source of nutrients and liquids, as well as any exogenous microorganisms colonising their intestine. Here we describe the unexpected finding of several monoxenous trypanosomatids in their gut. In a total of 564 individually examined Glossina (Austenia) tabaniformis (Westwood) (436 specimens) and Glossina (Nemorhina) fuscipes fuscipes (Newstead) (128 specimens) captured in the Dzanga-Sangha Protected Areas, Central African Republic, 24 (4.3%) individuals were infected with monoxenous trypanosomatids belonging to the genera Crithidia Léger, 1902; Kentomonas Votýpka, Yurchenko, Kostygov et Lukeš, 2014; Novymonas Kostygov et Yurchenko, 2020; Obscuromonas Votýpka et Lukeš, 2021; and Wallacemonas Kostygov et Yurchenko, 2014. Moreover, additional 20 (3.5%) inspected tsetse flies harboured free-living bodonids affiliated with the genera Dimastigella Sandon, 1928; Neobodo Vickerman, 2004; Parabodo Skuja, 1939; and Rhynchomonas Klebs, 1892. In the context of the recently described feeding behaviour of these dipterans, we propose that they become infected while taking sugar meals and water, providing indirect evidence that blood is not their only source of food and liquids.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Ultrastructure and molecular phylogeny of four new species of monoxenous trypanosomatids from flies (Diptera: Brachycera) with redefinition of the genus Wallaceina

Creator:
Yurchenko, Vyacheslav, Votýpka, Jan, Tesařová, Martina, Klepetková, Helena, Kraeva, Natalya, Jirků, Milan, and Lukeš, Julius
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Kinetoplastida, Trypanosomatidae, monoxenous kientoplastids, Leishmaniinae, molecular taxonomy, and phylogeny
Language:
English
Description:
Four new species of monoxenous kinetoplastid parasites are described from Brachycera flies, namely Wallaceina raviniae Votýpka et Lukeš, 2014 and Crithidia otongatchiensis Votýpka et Lukeš, 2014 from Ecuador, Leptomonas moramango Votýpka et Lukeš, 2014 from Madagascar, and Crithidia pragensis Votýpka, Klepetková et Lukeš, 2014 from the Czech Republic. The new species are described here based on sequence analysis of their spliced leader (SL) RNA, glycosomal glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) and small subunit (SSU) rRNA genes, as well as their morphology and ultrastructure. High-pressure freezing and Bernhard's EDTA regressive staining, used for the first time for monoxenous (one host) trypanosomatids, revealed the presence of viral particles with cytosolic localization in one and unique mitochondrial localization in another species. In accordance with previous observations, our results emphasize a discrepancy between morphology and molecular taxonomy of the family Trypanosomatidae. All four newly described species are represented by typical morphotypes (mainly choano- and promastigotes) and are virtually indistinguishable from other monoxenous trypanosomatids by morphology. Nevertheless, they all differ in their phylogenetic affinities. Whereas three of them grouped within the recently defined subfamily Leishmaniinae, which includes numerous representatives of the genera Leishmania Ross, 1903, Crithidia Léger, 1902 and Leptomonas Kent, 1880, the fourth species clusters together with the ''collosoma'' clade (named after ''Leptomonas'' collosoma Wallace, Clark, Dyer et Collins, 1960). Here we demonstrate that the ''collosoma'' group represents the elusive genus Wallaceina Podlipaev, Frolov et Kolesnikov, 1999. We redefine this genus in molecular terms based on similarities of the respective molecular markers and propose to use this taxon name for the group of species of the ''collosoma'' clade.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public