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Start Over Creator Lacinová, Z.

2. Increased intestinal permeability in patients with short bowel syndrome is not affected by parenteral nutrition

Creator:
Cinkajzlová, A., Lacinová, Z., Kloučková, J., Kaválková, P., Kratochvílová, H., Křížová, J., Trachta, P., Mráz, M., and Haluzík, M.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
short bowel syndrome, gut leakage, gut damage, and lipopolysaccharide
Language:
English
Description:
The aim of our study was to assess the presence and degree of intestinal leakage in subjects suffering from short bowel syndrome (SBS) and its modification by parenteral nutrition. To this end we assessed circulating levels of selected makers of intestinal permeability including zonulin, fatty acid binding protein 2 (FABP-2), citrulline and glucagon-like peptide 2 (GLP-2). We also measured lipopolysaccharide binding protein (LBP) as a marker of circulating levels of lipopolysaccharide acting through the CD14 molecule. Eleven SBS and 10 age- and BMI-matched control subjects were included into the study. The effect of parenteral nutrition was assessed after 14 days, 6 and 12 months from its initiation, respectively. At baseline, SBS patients had increased gut permeability as measured by zonulin (47.24±2.14 vs. 39.48±1.20 ng/ml, p=0.006) and LBP (30.32±13.25 vs. 9.77±0.71 µg/ml, p<0.001) compared to healthy controls. Furthermore, SBS subjects had reduced FABP-2, unchanged citrulline and increased sCD14 and GLP-2 relative to control group. Throughout the whole study period the administered parenteral nutrition had no significant effect on any of the studied parameters. Taken together, our data show that patients with short bowel syndrome have increased intestinal permeability that is not affected by parenteral nutrition.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

3. Insulin-like growth factor axis in pregnancy and gestational diabetes mellitus

Creator:
Anderlová, K., Cinkajzlová, A., Šimják, P., Kloučková, J., Kratochvílová, H., Lacinová, Z., Kaválková, P., Krejčí, H., Mráz, M., Pařízek, A., Haluzík, M., and Kršek , M.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
insulin-like growth factor, IGF-binding proteins, gestational diabetes mellitus, insulin sensitivity, and adipose tissue
Language:
English
Description:
The insulin-like growth factor (IGF) is involved in the regulation of growth and metabolism. The aim of this study was to determine selected parameters of IGF system at systemic and local levels [subcutaneous (SAT) and visceral adipose tissue (VAT)] to assess its possible role in gestational diabetes mellitus (GDM). 37 pregnant women (21 with GDM and 16 without GDM) and 15 age-matched non-pregnant females were included in the study. Blood samples were taken in 28-32 and 36-38 weeks of gestation and 6-12 months after delivery. SAT and VAT samples were obtained during delivery or surgery. Compared with nonpregnant women, serum IGF-1 and IGFBP-3 were increased in both groups of pregnant women. IGF-2 was elevated only in GDM women from 36 weeks of gestation culminating 6 months after delivery (p=0.003). Serum IGFBP-3 was increased and IGFBP-4 decreased in GDM women vs. pregnant women without GDM during the whole study (IGFBP-3: p˂0.001 for GDM vs. non-GDM; IGFBP-4: p=0.004 for GDM vs. non-GDM). Pregnant women with GDM had decreased mRNA expression of IGF-1, IGF-1R and IGF-2R and IGFBP-4 in VAT and IGF-1R in SAT compared to pregnant women without GDM. Changes in local activity of IGF are associated with the development of GDM.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

4. Plasma Levels of Total and Active Ghrelin in Acromegaly and Growth Hormone Deficiency

Creator:
Jarkovská, Z., Rosická, M., Marek, J., Hána, V., Weiss, V., Justová, V., Lacinová, Z., Haluzík, and Kršek, M.
Type:
article, model:article, and TEXT
Subject:
Total ghrelin, Active ghrelin, Leptin, Acromegaly, and Growth hormone deficiency
Language:
English
Description:
Ghrelin is an endogenous growth hormone (GH) secretagogue recently isolated from the stomach. Although it possesses a strong GH releasing activity in vitro and in vivo, its physiological significance in endogenous GH secretion remains unclear. The aim of this study was to characterize plasma ghrelin levels in acromegaly and growth hormone deficiency (GHD). We investigated plasma total and active ghrelin in 21 patients with acromegaly, 9 patients with GHD and 24 age-, sex- and BMI-matched controls. In all subjects, we further assessed the concentrations of leptin, soluble leptin receptor, insulin, IGF-I, free IGF-I and IGFBP-1, 2, 3 and 6. Patients with acromegaly and GHD as well as control subjects showed similar levels of total ghrelin (controls 2.004±0.18 ng/ml, acromegalics 1.755±0.16 ng/ml, p=0.31, GHD patients 1.704±0.17 ng/ml, p=0.35) and active ghrelin (controls 0.057±0.01 ng/ml, acromegalics 0.047±0.01 ng/ml, p=0.29, GHD patients 0.062±0.01 ng/ml, p=0.73). In acromegalic patients plasma total ghrelin values correlated negatively with IGF-I (p<0.05), in GHD patients active ghrelin correlated with IGF-I positively (p<0.05). In the control group, total ghrelin correlated positively with IGFBP-2 (p<0.05) and negatively with active ghrelin (p=0.05), BMI (p<0.05), WHR (p<0.05), insulin (p=0.01) and IGF-I (p=0.05). Plasma active ghrelin correlated positively with IGFBP-3 (p=0.005) but negatively with total ghrelin and free IGF-I (p=0.01). In conclusion, all groups of the tested subjects showed similar plasma levels of total and active ghrelin. In acromegaly and growth hormone deficiency plasma ghrelin does not seem to be significantly affected by changes in GH secretion.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

5. The effect of very-low-calorie diet on mitochondrial dysfunction in subcutaneous adipose tissue and peripheral monocytes of obese subjects with type 2 diabetes mellitus

Creator:
Urbanová, M., Mráz, M., Ďurovcová, V., Trachta, P., Kloučková, J., Kaválková, P., Haluzíková, D., Lacinová, Z., Hansíková, H., Wenchich, L., Kršek, M., and Haluzík, M.
Type:
article, model:article, and TEXT
Subject:
mitochondrial dysfunction, obesity, type 2 diabetes mellitus, very low-calorie diet, and peripheral monocytes
Language:
English
Description:
Mitochondrial dysfunction is a potentially important player in the development of insulin resistance and type 2 diabetes mellitus (T2DM). We investigated the changes of mRNA expression of genes encoding main enzymatic complexes of mitochondrial respiratory chain in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM) of 11 subjects with simple obesity (OB), 16 obese patients with T2DM and 17 healthy lean subjects (C) before and after very low-calorie diet (VLCD) using quantitative real time PCR. At baseline in SCAT, both T2DM and OB group had decreased mRNA expression of all investigated mitochondrial genes with the exception of 2 complex I (NDUFA 12) and complex IV (COX 4/1) enzymes in OB subjects. In contrast, in PM only the expression of complex I enzymes NDUFA 12 and MT-ND5 was reduced in both T2DM and OB subjects along with decreased expression of citrate synthase (CS) in T2DM group. Additionally, T2DM subjects showed reduced activity of pyruvate dehydrogenase and complex IV in peripheral blood elements. VLCD further decreased mRNA expression of CS and complex I (NT-ND5) and II (SDHA) enzymes in SCAT and complex IV (COX4/1) and ATP synthase in PM of T2DM group, while increasing the activity of complex IV in their peripheral blood elements. We conclude that impaired mitochondrial biogenesis and decreased activity of respiratory chain enzymatic complexes was present in SCAT and PM of obese and diabetic patients. VLCD improved metabolic parameters and ameliorated mitochondrial oxidative function in peripheral blood elements of T2DM subjects but had only minor and inconsistent effect on mitochondrial gene mRNA expression in SCAT and PM.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public