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Start Over Subject Apolipoprotein E

2. Differences in Anxiety-Related Behavior Between Apolipoprotein E-Deficient C57BL/6 and Wild Type C57BL/6 Mice

Creator:
McLachlan, C.S. and Yi Xing Soh, C.
Type:
article, model:article, and TEXT
Subject:
Apolipoprotein E, Anxiety, Elevated plus maze, and Mice
Language:
English
Description:
The influence of ApoE gene deletion on the anxiety state has not been previously investigated. The elevated plus maze was used in this study to determine differences in anxiety-related behavior between apoE-deficient and wild type C57BL/6 mice. The apoE-deficient mice demonstrated less anxiety on the elevated plus maze by spending more time in the open arms of the elevated plus maze compared to wild type mice (p<0.001). Additionally, female apoE-deficient mice visited the open arm of the maze more often than their apoE-deficient male counterpart (p<0.05). The anxiety state and/or sex are possible variables to be considered when designing physiological and/or behavioral studies involving mice that are apoE-deficient.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Effect of Diets on Lipoprotein Concentrations in Heterozygous Apolipoprotein E-Deficient Mice

Creator:
Bobková, D., Honsová, E., Kovář, J., and Poledne, R.
Type:
article, model:article, and TEXT
Subject:
Apolipoprotein E, Heterozygous mice, Hyperlipoproteinemia, and Hepatotoxicity
Language:
English
Description:
Loss of apolipoprotein E synthesis causes increased serum cholesterol concentrations and the sensitivity to high-fat diet in mice. We analyzed the changes in lipoprotein and hepatic structures in apolipoprotein E-deficient mice kept on control diet and cholesterol diets. Basal cholesterolemia of heterozygous (+/-) mice (2.2±0.28 mmol/l) was the same compared to wild-type (+/+) mice (2.3±0.15 mmol/l), but was lower compared to homozygous (-/-) mice (10.3±1.40 mmol/l). In +/- mice, cholesterolemia rose to 3.2 mmol/l on cholesterol diet and to 9 mmol/l on cholate diet, to 3 mmol/l and 3.6 mmol/l in +/+ mice, and to 23.4 mmol/l and 70.5 mmol/l in -/- mice, respectively. While the ratio of cholesterol/triglyceride concentrations in VLDL, IDL and LDL fractions was not increased in +/- mice and +/+ mice, it was increased in -/- mice on control diet. On the cholesterol diet, this ratio rose and was dramatically increased by cholate diet in all groups of mice. Even though cholate supplementation increased cholesterol concentration, it led to substantial toxic changes in hepatic morphology of all animals. In conclusion, one functional apo E allele in +/- mice is effective in keeping serum cholesterol concentrations in normal range on a control diet, but not on the cholesterol and cholate diets.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Rare Variant of Apolipoprotein E (Arg136->Cys) in a Subject with Normal Lipid Values

Creator:
Hubáček, J. A., Jan Piťha, Zdenka Škodová, and Rudolf Poledne
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Apolipoprotein E, Rare mutation, Lipid metabolism, 14, and 612
Language:
English
Description:
During the screening of apolipoprotein (apo) E gene polymorphism with PCR and subsequent restriction analysis, we have identified a female carrier with a mutant allele Arg136 ® Cys. This proband had normal lipid parameters and no history of coronary artery disease (CAD). We did not confirm the previously described connection between apo E Arg136 ® Cys mutation and elevated lipid levels. In the case of this mutation, other factors (environmental and/or genetic) are important for the development of lipid metabolism disorders., J. A. Hubáček, J. Piťha, Z. Škodová, R. Poledne., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

5. Rare Variant of Apolipoprotein E (Arg136→Ser) in Two Normolipidemic Individuals

Creator:
Hubáček, J.A., Adámková, V., and Škodová, Z.
Type:
article, model:article, and TEXT
Subject:
Apolipoprotein E, Rare mutation, and Lipid metabolism
Language:
English
Description:
Through the analysis of the common apolipoprotein (apo) E gene polymorphism in large Caucasian population study with the PCR and subsequent restriction analysis, we have identified carriers of mutant allele Arg136→Ser. Both of them (71-years-old female and her 43-years-old son) have normal lipid parameters. We suggest that Arg136→Ser mutation in apoE is not necessarily connected with elevated lipid levels in all cases. Furthermore, so far unidentified factors (environmental and/or genetic) are important for the development of lipid metabolism disorders in apoE Arg136→Ser mutation carriers.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public