We report on the first field season at the Gravettian site Hošťálkovice II. Hošťálkovice II is an important site in the region of Ostrava situated at a strategic position above the confluence of Odra and Opava Rivers. Previous surface prospections and a small test pit excavated in 1995 found evidence for a Gravettian industry, as well as younger (Neolithic?) occupation. Two archaeological layers were detected in 1995 and the documented profile was 1.3–1.5 m thick. An excavation conducted in 2019 approx. 30 m away from the 1995 test pit showed significant differences in stratigraphy. The maximum thickness of the sediments does not exceed 30 cm. Both Palaeolithic (patinated) and post-Paleolithic (non-patinated) artefacts were situated throughout the profile. In this contribution, we present all previous knowledge about the site, discuss the stratigraphy and the possibility of several occupation events at the site during the Palaeolithic/post-Palaeolithic period.