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Start Over Subject cadmium toxicity

1. Effect of cadmium on organization and photoreduction of protochlorophyllide in dark-grown leaves and etioplast inner membrane preparations of wheat

Creator:
Boddi, B., A.R., and Lehoczki, E.
Format:
print
Type:
model:internalpart and TEXT
Subject:
cadmium toxicity, Triticum, phototransformation, NADPH, fluorescence emission spectra, and chlorophyllide
Language:
Multiple languages
Description:
The cadmium treatment of dark-grown leaves and isolated etioplast inner membranes of wheat resulted in a decrease of the amount of the 657 nm emitting (77 K fluorescence) protochlorophyllide (PChlide) form, a simultaneous increase of the 633 nm form and the appearance of a 641.5 nm emitting form. This effect did not occur if excess NADPH was added to the isolated membranes: these samples showed spectral properties identical to those of non-treated (control) samples. Inhibition of the PChlide phototransformation was observed in the cadmium-treated leaves and membranes, the irradiation resulted in the appearance of a smáli amount of chlorophyllide (Chlide) with characteristic emission band at 678 nm. If excess NADPH was added, the inhibition did not occur and flash irradiation resulted in formation of the 694 nm Chlide form similarly as in control plants.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Transient increase in cellular dehydrogenase activity after cadmium treatment precedes enhanced production of reactive oxygen species in human proximal tubular kidney cells

Creator:
Handl, J. , Čapek, J., Majtnerová, P. , Petira, F. , Hauschke, M. , Roušarová, E., and Roušar , T.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
cadmium toxicity, kidney injury, dehydrogenase activity, oxidative stress, and ROS production
Language:
English
Description:
Cadmium is a heavy metal causing toxicity especially in kidney cells. The toxicity is linked also with enhanced oxidative stress leading to cell death. On the other hand, our recent experiments have shown that an increase of total intracellular dehydrogenases activity can also occur in kidney cells before declining until cell death. The aim of the present study, therefore, was to evaluate this transient enhancement in cell viability after cadmium treatment. The human kidney HK-2 cell line was treated with CdCl2 at concentrations 0-200 µM for 2-24 h and intracellular dehydrogenase activity was tested. In addition, we measured reactive oxygen species (ROS) production, glutathione levels, mitochondrial membrane potential, and C-Jun-N-terminal kinase (JNK) activation. We found that significantly increased dehydrogenase activity could occur in cells treated with 25, 100, and 200 µM CdCl2. Moreover, the results showed an increase in ROS production linked with JNK activation following the enhancement of dehydrogenase activity. Other tests detected no relationship with the increased in intracellular dehydrogenase activity. Hence, the transient increase in dehydrogenase activity in HK-2 cells preceded the enhancement of ROS production and our finding provides new evidence in cadmium kidney toxicity.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public