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1. A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity

Creator:
Choo, Young Moo, Lee, Kwang Sik, Yoon, Hyung Joo, Lee, Sang Beom, Kim, Ji Hyeon, Sohn, Hung Dae, and Jin, Byung Rae
Type:
article, model:article, and TEXT
Subject:
Apidae, baculovirus expression vector, Bombus ignitus, bumblebee, cDNA cloning, enzyme, expression, gene structure, and serine protease
Language:
English
Description:
A serine protease gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus serine protease (BiSP) gene spans 1702 bp and consists of four introns and five exons coding for 250 amino acid residues. Southern blot analysis of genomic DNA suggested that BiSP gene is a single copy gene. The cDNA encoding BiSP was expressed as a 28-kDa polypeptide in baculovirus-infected insect cells and the recombinant BiSP showed activity in a protease enzyme assay. BiSP was specifically expressed in the midgut of B. ignitus queens, males, and workers, suggesting that the BiSP is a gut enzyme involved in the digestion of dietary proteins.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

2. Expression of serotonin receptors in mouse oocytes and preimplantation embryos

Creator:
Veselá, J., Rehák, P., Mihalik, J., Soňa Czikková, Pokorný, J., and Juraj Koppel
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, expression, oocyte, preimplantation, serotonin, sumatriptan, 14, and 612
Language:
English
Description:
Serotonin receptors have been found in several reproductive organs as well as in the central nervous system. Serotonin-binding sites have been demonstrated in duck ovarian follicles and the testis, hamster ovaries, human granulosa cells and mouse placenta. Local production of serotonin by the rat ovary, oviduct, uterus and testis has also been reported. We analyzed the expression of three types of serotonin receptors: 5-HT1B, 5-HT2C and 5-HT1D by reverse transcription-polymerase chain reaction in mouse unfertilized oocytes and preimplantation embryos from zygotes to the blastocyst stage in vivo. Transcripts for 5-HT1B and 5-HT2C serotonin receptors were detected neither in unfertilized oocytes nor at any stages of in vivo developing preimplantation embryos. Serotonin 5-HT1D receptor mRNA was present in unfertilized oocytes, zygotes, 2-cell embryos, compacted morulae and in vivo produced expanded blatocysts. The expression of the mRNA 5-HT1D serotonin receptor was also detected in blastocysts cultured in vitro. When added to the culture medium, specific serotonin 5-HT1D agonist sumatriptan (1 μM) significantly inhibited the development of mouse embryos cultured in vitro. Demonstration of the expression of 5-HT1D serotonin receptor in mouse oocytes and preimplantation embryos supports the idea of a functional serotonin (5-HT1D) receptor in early mammalian development., J. Veselá, P. Rehák, J. Mihalik, S. Czikková, J. Pokorný, J. Koppel., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Must expression be instrumental?

Creator:
Bäckström, Stina
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
expression, Green, Merleau-Ponty, signal, and Wittgenstein
Language:
Slovak
Description:
This article engages critically with the theory of expression proposed by Mitchell S. Green in his Self-Expression (2007). In this book, Green argues that expressions are signals designed to convey information about mental states. By putting pressure on one of the examples Green uses in his book, I will challenge this thesis. I will then deepen this challenge by developing a contrast between two philosophical perspectives on expression, which I name the 'instrumental' and the 'descriptive'. I take Green’s theory of expression to be an exemplar of the instrumental perspective. Expression, in the instrumental perspective, is a means for transmitting information about mental states from organism to organism. The descriptive perspective I articulate with the help of Maurice Merleau-Ponty and Ludwig Wittgenstein. On the descriptive view, expression is (at least a part of) an answer to the question what it is so much as to have mental states and a living body. I suggest at the end of the article that if we remain within the instrumental perspective, we will not be able to use expression to satisfactorily answer this question.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public