The K13 propeller domain mutation and pfmdr1 amplification have been proposed as useful molecular markers for detection and monitoring of artemisinin resistant Plasmodium falciparum Welch, 1897. Genomic DNA isolates of P. falciparum was extracted from 235 dried blood spot or whole blood samples collected from patients with uncomplicated falciparum malaria residing in areas along the Thai-Myanmar border during 2006-2010. Nested polymerase chain reaction (PCR) and sequencing were performed to detect mutations in K13 propeller domain of P. falciparum at codon 427-709. Pfmdr1 gene copy number was determined by SYBR Green I real-time PCR. High prevalence of pfmdr1 multiple copies was observed (42.5% of isolates). The presence of K13 mutations was low (40/235, 17.2%). Seventeen mutations had previously been reported and six mutations were newly detected. The C580Y was found in two isolates (0.9%). The F446I, N458Y and P574L mutations were commonly detected. Seven isolates had both K13 mutation and pfmdr1 multiple copies. It needs to be confirmed whether parasites harbouring both K13 mutation and pfmdr1 multiple copies and/or the observed new mutations of K13 propeller domain are associated with clinical artemisinin resistance., Papichaya Phompradit, Wanna Chaijaroenkul, Phunuch Muhamad, Kesara Na-Bangchang., and Obsahuje bibliografii
Wenzel Trnka von Krzowitz was born in 1739 in Tabor. He graduated from the University of Vienna, where after studying philosophy he studied at the Faculty of Medicine. His personality and systematic work attracted the attention of Gerard van Swieten, who in 1769 made a significant contribution to the institutional establishment of the first medical faculty in the Hungarian Lands. Trnka thus became one of the founding members of this faculty, where he was appointed professor of anatomy. The Faculty of Medicine was the last part of the Pázmany University to be established in Trnava, which could not meet the needs of a growing university, especially of the medical faculty itself, and so in 1777 the entire campus was moved to Buda. While still in Trnava, in 1775, Trnka published one of his most important works, Historia febrium intermittentium, in which he discusses intermittent fevers. These fevers were a relatively common and unpleasant phenomenon in Europe, especially in certain regions. They are caused by protozoa of the genus Plasmodium, discovered in the 19th century, which cause several types of malaria, all of them being characterised by periodic bouts of fever. In his work, Trnka discusses in detail both the actual course of the disease and the treatment, emphasizing the use of quinine bark. The work contains several historically valuable chapters. It describes views and treatments of malaria in the 18th century, focusing also on those areas in the Habsburg Monarchy where the disease was widespread. Through Trnka’s work, the article provides an insight into life with this now exotic disease, which is today of little concern in our part of the world.