Our study was aimed to characterize the phenotype and functional endpoints of local microwave hyperthermia (LHT, 42 °C) on tumor infiltrating and spleen leukocytes. The effectiveness of LHT applied into the tumor of B16F10 melanoma-bearing C57/BL6 mice was compared with anesthetized and non-treated animals. Subpopulations of leukocytes were analyzed using the flow cytometry, and the cytotoxic activity of splenocytes against syngeneic B16F10 melanoma and NK-sensitive YAC-1 tumor cell lines was evaluated in 51 Cr-release assay. Similarly, the in vitro modification of the heat treatment was performed using healthy and melanoma-bearing splenocytes. We found a 40 % increase of activated monocytes (CD11b+CD69+) infiltration into the tumor microenvironment. In the spleen of experimental animals, the numbers of cytotoxic T lymphocytes (CTLs-CD3+CD8+) and NK cell (CD49b+NK1.1+) raised by 22 % and 14 %, respectively, while the NK1.1+ monocytes decreases by 37 %. This was accompanied by an enhancement of cytotoxic effector function against B16F10 and YAC-1 targets in both in vivo and in vitro conditions. These results demonstrate that LHT induces better killing of syngeneic melanoma targets. Furthermore, LHT evokes the homing of activated monocytes into the tumor microenvironment and increases the counts of NK cells and CTL in the spleen., J. Kubeš, J. Svoboda, J. Rosina, M. Starec, A. Fišerová., and Obsahuje bibliografii a bibliografické údaje
Část lékařské biofyziky studuje působení fyzikálních faktorů na zdraví člověka. Významným faktorem životního prostředí je ultrafialová složka slunečního záření, která hraje důležitou roli při vzniku a rozvoji maligního melanomu. Dalším významným rizikovým faktorem životního prostředí je degradace ozonové vrstvy, zejména ve stratosféře., There is a branch of medical biophysics studying the impact of physical factors on human health. The important environmental factor playing a significant role in formation and development of cutaneous melanoma is ultraviolet solar radiation. This risk factor is enhanced by the depletion of the ozone layer., Jozef Rosina, Jana Vránová, Kateřina Olefirenko., and Obsahuje bibliografii