The diet of otters Lutra lutra was studied by spraint analysis in six river and stream habitats in eastern Poland. In all of them fish were the staple food of otters, constituting from 29 % to 96 % of prey consumed in particular seasons. In upland (river Tanew) and mountain (river San, streams Dwernik and Głęboki) habitats, bullheads (Cottus gobio) were the most frequently eaten fish throughout the year (with maximum contribution of 57 % in the winter diet on river Tanew). In these habitats otters preyed on total of 10 fish species, including: brown trout (Salmo trutta), stone loach (Barbatula barbatula ), brook minnow (Phoxinus phoxinus) and stickleback (Gasterosteus aculeatus). On the lowland rivers, otters relied on more fish species than on the upland and mountain rivers (14 species on river Czarna Hańcza and 12 species on the upper and lower course of river Biebrza). Beside various cyprinids, otters preyed on stickleback, perch (Perca fluviatilis), mud loach (Misgurnus fossilis), burbot (Lota lota) and pike (Esox lucius). On all rivers, except the lower Biebrza, most of fish (from 70% to 88%) captured by otters were less than 10 cm long. Amphibians and crayfish were two other important groups of prey. On small streams in the Bieszczady Mountains, frogs (mainly Rana temporaria) comprised up to 46% of otter prey. Crayfish (Ortonectes limosus) remains were recorded in otter spraints only on the lowland rivers: in summer crayfish comprised 52% of prey on the Czarna Hańcza River and 47% of prey on the upper Biebrza River. In upland and mountain habitats the food niche breadth of otters was lower (from B=2.62 to B=6.12) than on the lowland rivers (from B=3.10 to B=11.09) and did not differ much between the seasons. On the lowland rivers the food niche breadth was high in winter and low in summer, and the seasonal differences in otter diet were much more pronounced as compared to the upland and mountain habitats.
Aphidophagous ladybirds exhibit a broad range of body sizes. Until now this has been thought to be a function of the different prey densities that they feed at, with smaller ladybirds feeding at lower prey densities. The size of the prey species they feed on has been considered to have no relationship with ladybird body size. However, these arguments possess a limited capacity to explain observed data from the field. I here demonstrate a more realistic, complex approach incorporating both prey density and the size of prey species. Small ladybirds can feed on small aphids at both low and high densities. However when the aphid species is large they cannot catch the older, bigger, more energy-rich aphid instars due to their small size. They are thus unable to feed on large aphid prey at low densities, although at higher densities numbers of the smaller instars may be sufficient to sustain them. By contrast large ladybirds can feed on large aphids at both low and high densities due to their superior ability to catch the bigger, more energy-rich older aphids; however they cannot be sustained by low densities of small aphids due to food limitation consequent on their large size. This more complex association between ladybird size, prey size and prey density possesses a better explanatory power for earlier field data. Because of this relationship, ladybird body size also provides an important trade-off determining dietary breadth and specialization in the aphidophagous Coccinellidae. Dietary specialists more closely match the size of their limited prey species, have higher overall capture efficiencies and can thus continue to reproduce at lower aphid densities for longer. By contrast dietary generalists adopt a one-size-fits-all strategy, are medium-sized and have lower capture efficiencies of individual prey species, thus requiring higher aphid densities. The role of body-size in dietary specialization is supported by data from the British fauna. Rather than trade-offs related to prey chemistry, which have hitherto been the centre of attention, body size trade-offs are the likely most important universal factor underlying dietary specialization in aphidophagous coccinellids.