Effects of rapid or slow body weight reduction on intramuscular protein degradation pathways during equivalent weight loss on rats

Title:

Effects of rapid or slow body weight reduction on intramuscular protein degradation pathways during equivalent weight loss on rats

Creator:

Nonaka, Y., Urashima, S., Inai, M., Nishimura, S., Higashida, K., and Terada, S.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:008b3eaf-876a-4687-afc1-cd1cc8568833
uuid:008b3eaf-876a-4687-afc1-cd1cc8568833

Subject:

skeletal muscle, fasting, autophagy-lysosome, calorie restriction, and ubiquitin-proteasome

Type:

article, model:article, and TEXT

Description:

The purpose of this study was to compare the effects of shortterm fasting-induced rapid weight loss with those of slower but equivalent body weight loss induced by daily calorie restriction on muscle protein degradation pathways and muscle protein content. Male Fischer rats were subjected to either 30 % calorie restriction for 2 weeks to slowly decrease body weight (Slow) or 3-day fasting to rapidly decrease body weight by a comparable level of that of the Slow group (Rapid). The final body weights were about 15 % lower in both the Slow and Rapid groups than in the Con group (p<0.001). The total protein content and wet weight of fast-twitch plantaris muscle, but not slow-twitch soleus muscle, were significantly lower in the Rapid group compared with the control rats fed ad libitum. Substantial increases in the expression ratio of autophagosomal membrane proteins (LC3-II/-I ratio) and polyubiquitinated protein concentration, used as biomarkers of autophagy-lysosome and ubiquitinproteasome activities, respectively, were observed in the plantaris muscle of the Rapid group. Moreover, the LC3-II/-I ratio and polyubiquitinated protein concentration were negatively correlated with the total protein content and wet weight of plantaris muscle. These results suggest that short-term fastinginduced rapid body weight loss activates autophagy-lysosome and ubiquitin-proteasome systems more strongly than calorie restriction-induced slower weight reduction, resulting in muscular atrophy in fast-twitch muscle.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2017 Volume:66 | Number:5

Harvested from:

CDK

Metadata only:

false