Factors affecting the function of the mitochondrial membrane permeability transition pore and their role in evaluation of calcium retention capacity values

Title:

Factors affecting the function of the mitochondrial membrane permeability transition pore and their role in evaluation of calcium retention capacity values

Creator:

Drahota, Zdeněk, Endlicher, René, Kučera, Otto, Rychtrmoc, David, and Červinková, Zuzana

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:01c3a3c6-5570-41f3-96ae-cde848232930
uuid:01c3a3c6-5570-41f3-96ae-cde848232930
doi:10.33549/physiolres.934391

Subject:

liver mitochondria, calcium retention capacity, and mitochondrial permeability transition pore

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Values of the calcium retention capacity (CRC) of rat liver mitochondria are highly dependent on the experimental conditions used. When increasing amounts of added calcium chloride are used (1.25-10 nmol), the values of the CRC increase 3-fold. When calcium is added in 75 s intervals, the CRC values increase by 30 % compared with 150 s interval additions. CRC values are not dependent on the calcium/protein ratio in the measured sample in our experimental design. We also show that a more detailed evaluation of the fluorescence curves can provide new information about mitochondrial permeability transition pore opening after calcium is added.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

491-499

Source:

Physiological research | 2020 Volume:69 | Number:3

Harvested from:

CDK

Metadata only:

false