Determination of brain water content by dry/wet weight measurement for the detection of experimental brain edema

Title:

Determination of brain water content by dry/wet weight measurement for the detection of experimental brain edema

Creator:

Kozler, Petr, Marešová, Dana, and Pokorný, Jaroslav

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:02475258-fbfd-4647-a7df-6a34fef75b3b
uuid:02475258-fbfd-4647-a7df-6a34fef75b3b
doi:10.33549/physiolres.934996

Subject:

brain water content, dry/wet weight measurement, cytotoxic edema, and vasogenic edema

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Brain edema is a fatal pathological state in which brain volume increases as a result of abnormal accumulation of fluid within the brain parenchyma. A key attribute of experimentally induced brain edema – increased brain water content (BWC) – needs to be verified. Various methods are used for this purpose: specific gravimetric technique, electron microscopic examination, magnetic resonance imaging (MRI) and dry/wet weight measurement. In this study, the cohort of 40 rats was divided into one control group (CG) and four experimental groups with 8 rats in each group. The procedure for determining BWC using dry/wet weight measurement was initiated 24 h after the completion of edema induction by the water intoxication method (WI group); after the intraperitoneal administration of Methylprednisolone (MP) together with distilled water during edema induction (WI+MP group); 30 min after osmotic blood brain barrier disruption (BBBd group); after injection of MP via the internal carotid artery immediately after BBBd (BBBd + MP group). While induction of brain edema (WI, BBBd) resulted in significantly higher BWC, there was no increase in BWC in the MP groups (WI+MP, BBBd+MP), suggesting a neuroprotective effect of MP in the development of brain edema.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

S277-S283

Source:

Physiological research | 2022 Volume:71 | Number:Supplement 2

Harvested from:

CDK

Metadata only:

false