Transport of an antihypoxic drug stobadine across the blood-brain barrier in rat striatum and its influence on catecholamine-oxidative current: A voltammetric study under normal and anoxic / ischaemic conditions

Title:

Transport of an antihypoxic drug stobadine across the blood-brain barrier in rat striatum and its influence on catecholamine-oxidative current: A voltammetric study under normal and anoxic / ischaemic conditions

Creator:

Pavlásek, J., Haburčák, M., Mašánová, C., and Štolc, S.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:03554b40-5c4c-49ae-a32b-d61a89e7de49
uuid:03554b40-5c4c-49ae-a32b-d61a89e7de49

Subject:

blood-brain-barrier, stobadine, catecholamine, ischaemia, striatum, and rat

Type:

article, model:article, and TEXT

Description:

Differential pulse voltammetry with a carbon fibre microelectrode (ME) was used in pentobarbital- anaesthetized rats for monitoring the stobadine current (STB.C) on both sides of the blood-brain barrier (BBB) in the arterial bloodstream (BS) and in the corpus striatum (CS). The STB.C exhibited a distinct peak at a polarization voltage 540±30 mV (n=4). The maximum of STB.C in BS attained 2-3 min after the STB administration (2.8 mg/100 g in 1.0 ml saline solution i.a.) was followed by a rapid decrease to about 20 % within next 3 min. The STB readily passed across the BBB: the STB.C peak appeared in the CS in the 3rd minute and continued to rise up to the 30th min. The administration of STB did not prevent a large increase (1347±326 %, n=3) of the catechol-oxidative current (CA.OC) occurring in the CS between the 4th and 5th minute after cardiac arrest. However, a decrease of ME sensitivity to CA.OC in the presence of STB was observed. This fact leads to the speculation whether a similar "quenching" of dopamine by STB could not participate in the protective effects of STB observed in the brain exposed to hypoxia-reoxygenation.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1996 Volume:45 | Number:3

Harvested from:

CDK

Metadata only:

false