Simulated microgravity reduces proliferation and reorganizes the cytoskeleton of human umbilical cord mesenchymal stem cells

Title:

Simulated microgravity reduces proliferation and reorganizes the cytoskeleton of human umbilical cord mesenchymal stem cells

Creator:

Quynh Chi, Ho Nguyen, Nghia Son, Hoang, Chinh Chung, Doan, Dinh Huan, Le, Hong Diem, Tran, and Thanh Long, Le

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:08354f0b-1dbc-40d3-9944-609ae33c8753
uuid:08354f0b-1dbc-40d3-9944-609ae33c8753
issn:0862-8408
doi:10.33549/physiolres.934472

Subject:

proliferace buněk, cytoskelet, cell proliferation, cytoskeleton, human umbilical cord mesenchymal stem cells, simulated microgravity, 14, and 612

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

The cytoskeleton plays a key role in cellular proliferation, cellshape maintenance and internal cellular organization. Cells are highly sensitive to changes in microgravity, which can induce alterations in the distribution of the cytoskeletal and cell proliferation. This study aimed to assess the effects of simulated microgravity (SMG) on the proliferation and expression of major cell cycle-related regulators and cytoskeletal proteins in human umbilical cord mesenchymal stem cells (hucMSCs). A WST-1 assay showed that the proliferation of SMG-exposed hucMSCs was lower than a control group. Furthermore, flow cytometry analysis demonstrated that the percentage of SMG-exposed hucMSCs in the G0/G1 phase was higher than the control group. A western blot analysis revealed there was a downregulation of cyclin A1 and A2 expression in SMG-exposed hucMSCs as well. The expression of cyclin-dependent kinase 4 (cdk4) and 6 (cdk6) were also observed to be reduced in the SMG-exposed hucMSCs. The total nuclear intensity of SMG-exposed hucMSCs was also lower than the control group. However, there were no differences in the nuclear area or nuclear-shape value of hucMSCs from the SMG and control groups. A western blot and quantitative RT-PCR analysis showed that SMG-exposed hucMSCs experienced a downregulation of β-actin and α-tubulin compared to the control group. SMG generated the reorganization of microtubules and microfilaments in hucMSCs. Our study supports the idea that the downregulation of major cell cycle-related proteins and cytoskeletal proteins results in the remodeling of the cytoskeleton and the proliferation of hucMSCs., Ho Nguyen Quynh Chi, Hoang Nghia Son, Doan Chinh Chung, Le Dinh Huan, Tran Hong Diem, Le Thanh Long., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

897-906

Source:

Physiological research | 2020 Volume:69 | Number:5

Harvested from:

CDK

Metadata only:

false