Porcine dorsal root ganglia ovarian neurons are affected by long lasting testosterone treatment

Title:

Porcine dorsal root ganglia ovarian neurons are affected by long lasting testosterone treatment

Creator:

Jana, B., Palus, K., Meller, K., and Całka, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:0b435263-8050-4391-a320-4cd2f7b48b22
uuid:0b435263-8050-4391-a320-4cd2f7b48b22

Subject:

Testosterone, Dorsal root ganglia, Ovarian neurons, and Gilts

Type:

article, model:article, and TEXT

Description:

We studied the effect of testosterone overdose on the number, distribution and chemical coding of ovarian neurons in the dorsal root ganglia (DRGs) in pigs. On day 3 of the estrous cycle, the ovaries of both the control and experimental gilts were injected with retrograde tracer Fast Blue. From day 4 of the estrous cycle to the expected day 20 of the second studied cycle, the experimental gilts were injected with testosterone, while the control gilts received oil. After the completion of the protocol the Th16-L5 DRGs were collected. Injections of testosterone increased the testosterone (~3.5 fold) and estradiol-17β (~1.6 fold) levels in the peripheral blood, and reduced the following in the DRGs: the total number of the Fast Blue-positive perikarya, the population of perikarya in the L2-L4 ganglia, and the numbers of SP+/CGRP+, SP+/PACAP+, SP+/nNOS+ and SP-/nNOS+ perikarya. In thetestosterone-injected gilts, the populations of SP+/CGRP-, small and large androgen receptors-expressing perikarya were increased. These results suggest that elevated androgen levels during pathological states may regulate the transmission of sensory modalities from the ovary to the spinal cord, and antidromic regulation of the ovarian functions.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2016 Volume:65 | Number:6

Harvested from:

CDK

Metadata only:

false