Vitamin D upregulates endothelin-1, ETBR, eNOS mRNA expression and attenuates vascular remodeling and ischemia in kidney fibrosis model in mice

Title:

Vitamin D upregulates endothelin-1, ETBR, eNOS mRNA expression and attenuates vascular remodeling and ischemia in kidney fibrosis model in mice

Creator:

Arfian, N., Kusuma, M. H. H., Emoto, N., Ikeda, K., Suzuki, Y., Jeffilano, A., Nugroho, D. B., and Anggorowati, N.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:0dd166d1-860b-4118-ac8f-1210171c2bff
uuid:0dd166d1-860b-4118-ac8f-1210171c2bff
issn:0862-8408

Subject:

fyziologie člověka, human physiology, Kidney fibrosis, Endothelin-1, Vitamin D, Vascular remodeling, eNOS, 14, and 612

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

We examined the upregulation of ET-1/ETBR/eNOS signaling in renoprotective effect of vitamin D in kidney fibrosis model in mice using unilateral ureteral obstruction (UUO). One group was treated with intraperitoneal injection of 0.125 mg/kg of Calcitriol (UUO+VD). Vascular remodeling was quantified based on lumen area and lumen/wall area ratio (LWAR) of intrarenal arteries using Sirius Red staining. ET-1, ETBR, eNOS, CD31 and VEGF mRNA expressions were quantified using qRT-PCR. Focusing on endothelin-1 (ET-1) signaling in endothelial cells (EC), siRNA of ET-1 was performed in human umbilical vein endothelial cells (HUVEC) for reducing ET-1 expression. Then HUVECs were treated with and without 100 nM Calcitriol treatment in hypoxic and normoxic conditions to elucidate ET-1/eNOS signaling. Our in vivo study revealed vascular remodeling and renal ischemia attenuation after Calcitriol treatment. Vascular remodeling was attenuated in the UUO+VD group as shown by increasing lumen areas and LWAR in intrarenal arteries. These findings were associated with significant higher CD31 and VEGF mRNA expression compared to the UUO group. Vitamin D treatment also increased ET-1, ETBR and eNOS mRNA expressions. Our in vitro study demonstrated Calcitriol induced ET-1 and eNOS mRNA expressions upregulation in HUVEC under normoxic and hypoxic condition. Meanwhile, siRNA for ET-1 inhibited the upregulation of eNOS mRNA expression after Calcitriol treatment. Vitamin D ameliorates kidney fibrosis through attenuating vascular remodeling and ischemia with upregulating ET-1/ETBR and eNOS expression., N. Arfian, M. H. H. Kusuma, N. Anggorowati, D. B. Nugroho, A. Jeffilano, Y. Suzuki, K. Ikeda, N. Emoto., and Seznam literatury

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2018 Volume:67 | Number:Suppl 1

Harvested from:

CDK

Metadata only:

false