Long-term pioglitazone treatment augments insulin sensitivity and PKC-ε and PKC-θ activation in skeletal muscles in sucrose fed rats

Title:

Long-term pioglitazone treatment augments insulin sensitivity and PKC-ε and PKC-θ activation in skeletal muscles in sucrose fed rats

Creator:

Irena Marková, Václav Zídek, Alena Musilová, Miroslava Šimáková, Petr Mlejnek, Ludmila Kazdová, and Michal Pravenec

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:0e62edfe-1866-4c49-99dd-97d44c28d234
uuid:0e62edfe-1866-4c49-99dd-97d44c28d234

Subject:

Fyziologie člověka a srovnávací fyziologie, inzulinová rezistence, insulin resistance, novel PKC, pioglitazone, muscle lipids, rat, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

It has been suggested that thiazolidinediones (TZDs) ameliorate insulin resistance in muscle tissue by suppressing muscle lipid storage and the activity of novel protein kinase C (nPKC) isoforms. To test this hypothesis, we analyzed long-term metabolic effects of pioglitazone and the activation of nPKC-ε and -θ isoforms in an animal model of the metabolic syndrome, the spontaneously hypertensive rat (a congenic SHR strain with wild type Cd36 gene) fed a diet with 60 % sucrose from the age of 4 to 8 months. Compared to untreated controls, pioglitazone treatment was associated with significantly increased basal (809±36 vs 527±47 nmol glucose/g/2h, P<0.005) and insulinstimulated glycogenesis (1321±62 vs 749±60 nmol glucose/g/2h, P<0.0001) in isolated gastrocnemius muscles despite increased concentrations of muscle triglycerides (3.83±0.33 vs 2.25±0.12 μmol/g, P<0.005). Pioglitazone-treated rats exhibited significantly increased membrane/total (cytosolic plus membrane) ratio of both PKC-ε and PKC-θ isoforms compared to untreated controls. These results suggest that amelioration of insulin resistance after long-term pioglitazone treatment is associated with increased activation of PKC-ε and -θ isoforms in spite of increased lipid concentration in skeletal muscles., I. Marková ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:4

Harvested from:

CDK

Metadata only:

false