Morphological changes of rat jejunum after whole body γ-irradiation and their impact in biodosimetry

Title:

Morphological changes of rat jejunum after whole body γ-irradiation and their impact in biodosimetry

Creator:

Daniel Driák, Jan Österreicher, Jaroslava Vávrová, Zuzana Řeháková, and Zdena Vilasová

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:10d855af-bf63-4101-afca-ab50afc3c9cb
uuid:10d855af-bf63-4101-afca-ab50afc3c9cb
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, radiobiologie, enteritida, biologické markery, radiobiology, enteritis, biological markers, radiation-induced enteritis, treshold dose, biodosimetric marker, 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Gastrointestinal form is the second stage of the Acute Radiation Syndrome (ARS) with a threshold dose of 8 Gy. It represents an absolutely lethal clinical-pathological unit, enteritis necro-hemorrhagica (duodenitis, jejunitis, ileitis, respectively) with unknown causal therapy. The purpose of our study has been to evaluate the morphological changes in a model of radiation-induced enteritis in rats and estimate the significance of changes in biodosimetry. Wistar rats were randomly divided into 21 groups, 10 animals per group. Samples of the jejunum were taken 24, 48, 72, and 96 h after the whole-body γ-irradiation with the doses of 1, 5, 10, 15, and 20 Gy, and routinely stained with hematoxylin and eosin. Five morphometric markers – intercryptal distance, enterocytal height on the top and base of villus, length of basal lamina of 10 enterocytes and enterocytal width – in irradiated rat jejunum were examined. The results were compared with sham-irradiat ed control group. After lethal doses of irradiation, all morphometric parameters of jejunum significantly changed. With the exception of intercryptal distance, they might be considered as suitable biodosimetric markers under these experimental conditions. Our morphometry results in radiation-induced jejunitis are in accordance with those in other studies. We were the first who quantified morphological post-irradiation changes in animal jejunum. Some of them might be used under experimental conditions. This experimental study is a predecessor of the clinical assessment of a specific marker. Under clinical practice, the sensitive biodosimetric parameter could serve as one of the guidance for evaluation of the absorbed dose in irradiated troops as well as rescue workers. This is in accordance with tasks and Standardization Agreement of the North Atlantic Treaty Organization., D. Driák, J. Österreicher, J. Vávrová, Z. Řeháková, Z. Vilasová., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:3

Harvested from:

CDK

Metadata only:

false