Dopamine 2 and somatostatin 1-5 receptors coexpression in clinically non-functioning pituitar adenomas

Title:

Dopamine 2 and somatostatin 1-5 receptors coexpression in clinically non-functioning pituitar adenomas

Creator:

Filip Gabalec, Drastikova, M., Tomáš Česák, David Netuka, Václav Masopust, Josef Machač, Josef Marek, Jan Čáp, and Michal Beránek

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:136f4b56-1792-420c-8193-35aa0993d461
uuid:136f4b56-1792-420c-8193-35aa0993d461
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, dopaminové receptory, adenomy hypofýzy, imunohistochemie, dopamine receptors, pituitary adenomas, immunohistochemistry, somatostatin receptor, estrogen receptor, clinically non-functioning adenomas, real-time PCR, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

This study investigated quantitated expression of dopamine 2 receptor (D2R) and somatostatin receptors of the five types (SSTR1-SSTR5) in a large series of clinically non-functioning pituitary adenomas (CNFAs). Co-expression of these receptors in individual adenomas was studied as well as correlation between receptor types. Adenoma tissue from 198 patients who underwent surgery for CNFAs was analyzed by immunohistochemistry and quantitative real-time PCR. D2R and SSTR1-3 mRNA was expressed in all 198 adenomas. SSTR4 and SSTR5 were detectable in 85 % and 61 % of adenomas, respectively. Expression of D2R was significantly higher than that of the somatostatin receptors. The median relative expressions were as follows from highest D2R >> SSTR3 > SSTR2 > SSTR1 > SSTR5 > SSTR4. High relative expression (ratio to β-glucuronidase mRNA > 1) of D2R was found in 60 % of tumors, high expression of SSTR1 in 7.5 %, SSTR2 in 7 %, SSTR3 in 4 % and SSTR5 in 0.5 %. The quantity of D2R correlated positively with expression of SSTR2 and SSTR3, and negatively with SSTR1 and SSTR5. Among histological adenoma types, SSTR1 was significantly higher in null-cell adenomas and SSTR3 was lower in silent corticotroph adenomas. In conclusions, in CNFAs, high expression of somatostatin receptors is much less common than that of D2R, and co-expression of both these receptors is exceptional. D2R and SSTR3 seem to be the most promising targets for pharmacological treatment., F. Gabalec, M. Drastikova, T. Cesak, D. Netuka, V. Masopust, J. Machac, J. marek, J. Cap, M. Beranek., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:3

Harvested from:

CDK

Metadata only:

false